Detailed information    

insolico Bioinformatically predicted

Overview

Name   pilA2   Type   Machinery gene
Locus tag   ACM62G_RS07440 Genome accession   NZ_CP183808
Coordinates   1611921..1612319 (-) Length   132 a.a.
NCBI ID   WP_419736951.1    Uniprot ID   -
Organism   Pseudomonas sp. COR18     
Function   assembly of type IV pilus (predicted from homology)   
DNA binding and uptake

Related MGE

Note: This gene co-localizes with putative mobile genetic elements (MGEs) in the genome predicted by VRprofile2, as detailed below.

Gene-MGE association summary

MGE type MGE coordinates Gene coordinates Relative position Distance (bp)
Genomic island 1596010..1611802 1611921..1612319 flank 119


Gene organization within MGE regions


Location: 1596010..1612319
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  ACM62G_RS07395 (ACM62G_07395) - 1598015..1599547 (+) 1533 WP_419736945.1 NACHT domain-containing protein -
  ACM62G_RS07400 (ACM62G_07400) - 1603099..1603980 (-) 882 WP_419736946.1 hypothetical protein -
  ACM62G_RS07405 (ACM62G_07405) - 1604069..1605481 (-) 1413 Protein_1463 DUF927 domain-containing protein -
  ACM62G_RS07410 (ACM62G_07410) - 1605559..1605828 (-) 270 WP_375135886.1 helix-turn-helix transcriptional regulator -
  ACM62G_RS07415 (ACM62G_07415) - 1605875..1606831 (-) 957 WP_419736947.1 hypothetical protein -
  ACM62G_RS07420 (ACM62G_07420) - 1607122..1608417 (-) 1296 WP_419736948.1 tyrosine-type recombinase/integrase -
  ACM62G_RS07430 (ACM62G_07430) - 1608694..1609752 (-) 1059 WP_419736949.1 O-antigen ligase family protein -
  ACM62G_RS07435 (ACM62G_07435) - 1610156..1611799 (-) 1644 WP_419736950.1 hypothetical protein -
  ACM62G_RS07440 (ACM62G_07440) pilA2 1611921..1612319 (-) 399 WP_419736951.1 pilin Machinery gene

Sequence

Protein


Download         Length: 132 a.a.        Molecular weight: 13316.27 Da        Isoelectric Point: 7.5895

>NTDB_id=1106213 ACM62G_RS07440 WP_419736951.1 1611921..1612319(-) (pilA2) [Pseudomonas sp. COR18]
MNAQKGFTLIELMIVVAIIGILAAIALPAYNSYTQKARFAEVNTMADAYKTAVAVCINDLGTKTNCTAGSNGIPAIPAAT
PNFASGSVTDGTISLTGTTAAGGWTTILAPTLNAGNITWAQTGTCAAANTCK

Nucleotide


Download         Length: 399 bp        

>NTDB_id=1106213 ACM62G_RS07440 WP_419736951.1 1611921..1612319(-) (pilA2) [Pseudomonas sp. COR18]
ATGAATGCTCAAAAGGGTTTCACCTTGATCGAACTGATGATCGTCGTGGCGATCATCGGCATTTTGGCGGCGATCGCGTT
GCCTGCGTATAACAGCTATACGCAGAAGGCCCGCTTTGCCGAAGTGAATACTATGGCTGACGCCTATAAGACGGCCGTGG
CTGTCTGCATTAATGATCTAGGTACCAAAACCAACTGTACGGCAGGCTCCAATGGTATCCCTGCCATTCCTGCTGCCACT
CCTAACTTCGCGTCTGGTTCTGTGACTGACGGCACAATCAGTTTGACGGGGACTACTGCTGCGGGTGGCTGGACAACTAT
CCTCGCACCTACTCTCAACGCTGGTAATATCACTTGGGCTCAGACTGGTACCTGTGCCGCGGCTAACACCTGTAAATGA

Domains

Predicted by InterproScan.

Prokaryotic N-terminal methylation motif (N_methyl)

(1-27)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  pilA2 Legionella pneumophila strain ERS1305867

52.308

98.485

0.515
  pilA2 Legionella pneumophila str. Paris

52.308

98.485

0.515
  comP Acinetobacter baylyi ADP1

50.37

100

0.515
  pilA Ralstonia pseudosolanacearum GMI1000

42.143

100

0.447
  pilA Haemophilus influenzae 86-028NP

43.802

91.667

0.402
  pilA Glaesserella parasuis strain SC1401

43.802

91.667

0.402
  pilA Haemophilus influenzae Rd KW20

42.975

91.667

0.394

Multiple sequence alignment