Detailed information    

insolico Bioinformatically predicted

Overview

Name   pilA   Type   Machinery gene
Locus tag   ACN6IU_RS10505 Genome accession   NZ_CP183804
Coordinates   2065768..2066202 (+) Length   144 a.a.
NCBI ID   WP_005741096.1    Uniprot ID   -
Organism   Pasteurella multocida strain 171865     
Function   type IV pilus biogenesis and function (predicted from homology)   
DNA binding and uptake

Genomic Context

Location: 2060768..2071202
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  ACN6IU_RS10475 tadA 2060879..2061379 (-) 501 WP_016504277.1 tRNA adenosine(34) deaminase TadA -
  ACN6IU_RS10480 - 2061394..2062245 (-) 852 WP_005751072.1 thymidylate synthase -
  ACN6IU_RS10485 lgt 2062251..2063054 (-) 804 WP_005751073.1 prolipoprotein diacylglyceryl transferase -
  ACN6IU_RS10490 - 2063064..2063858 (-) 795 WP_005722415.1 sulfite exporter TauE/SafE family protein -
  ACN6IU_RS10495 rppH 2063861..2064457 (-) 597 WP_005722417.1 RNA pyrophosphohydrolase -
  ACN6IU_RS10500 ampD 2065043..2065609 (-) 567 WP_014326302.1 1,6-anhydro-N-acetylmuramyl-L-alanine amidase AmpD -
  ACN6IU_RS10505 pilA 2065768..2066202 (+) 435 WP_005741096.1 prepilin peptidase-dependent pilin Machinery gene
  ACN6IU_RS10510 pilB 2066202..2067587 (+) 1386 WP_016504529.1 GspE/PulE family protein Machinery gene
  ACN6IU_RS10515 - 2067587..2068798 (+) 1212 WP_014326300.1 type II secretion system F family protein -
  ACN6IU_RS10520 - 2068798..2069520 (+) 723 WP_014326299.1 prepilin peptidase -
  ACN6IU_RS10525 coaE 2069535..2070155 (+) 621 WP_014326298.1 dephospho-CoA kinase -
  ACN6IU_RS10530 yacG 2070166..2070369 (+) 204 WP_005719879.1 DNA gyrase inhibitor YacG -
  ACN6IU_RS10535 - 2070369..2070638 (+) 270 WP_005722568.1 GNAT family N-acetyltransferase -

Sequence

Protein


Download         Length: 144 a.a.        Molecular weight: 15058.48 Da        Isoelectric Point: 9.1281

>NTDB_id=1106198 ACN6IU_RS10505 WP_005741096.1 2065768..2066202(+) (pilA) [Pasteurella multocida strain 171865]
MKKAIFFSIKKGFTLIELMIVIAIIAILATIAVPSYQNYTKKAAISELLQAAAPYRAEVELCIYNTNSTTNCNAGSHGIR
ADAAASKKYLKAITVKAGVITVTGQGSLEGISYTLTASGNAAQGVSWTVNCGAQSDIFPAGFCA

Nucleotide


Download         Length: 435 bp        

>NTDB_id=1106198 ACN6IU_RS10505 WP_005741096.1 2065768..2066202(+) (pilA) [Pasteurella multocida strain 171865]
ATGAAAAAAGCCATTTTCTTTTCGATTAAAAAAGGGTTTACGCTAATTGAATTAATGATTGTCATTGCCATTATTGCGAT
TTTAGCCACGATTGCCGTGCCATCTTATCAAAATTATACAAAAAAAGCGGCAATCTCTGAATTATTGCAAGCCGCAGCTC
CTTATCGTGCTGAGGTAGAGCTTTGTATCTATAATACAAATAGTACAACGAATTGTAATGCAGGAAGTCATGGTATTCGT
GCTGATGCTGCAGCAAGTAAAAAATATTTAAAAGCCATCACGGTTAAAGCTGGGGTAATTACTGTCACTGGACAAGGGAG
CTTGGAAGGAATTAGCTATACGCTGACAGCGAGTGGTAACGCAGCTCAAGGTGTTTCTTGGACAGTTAATTGTGGTGCTC
AATCTGACATTTTCCCAGCAGGATTTTGCGCATAA

Domains

Predicted by InterproScan.

Pilin (bacterial filament) (Pilin)

(42-141)

Prokaryotic N-terminal methylation motif (N_methyl)

(10-32)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  pilA Haemophilus influenzae 86-028NP

63.194

100

0.632
  pilA Haemophilus influenzae Rd KW20

61.111

100

0.611
  pilA Glaesserella parasuis strain SC1401

52.349

100

0.542
  pilA Vibrio cholerae O1 biovar El Tor strain E7946

36.806

100

0.368
  pilA Vibrio cholerae strain A1552

36.806

100

0.368
  pilA Vibrio cholerae C6706

36.806

100

0.368

Multiple sequence alignment