Detailed information NTG1106199

Bioinformatically predicted

Overview

• Name: pilB
• Type: Machinery gene
• Locus tag: ACN6IU_RS10510
• Genome accession: NZ_CP183804
• Coordinates: 2066202..2067587 (+)
• Length: 461 a.a.
• NCBI ID: WP_016504529.1
• Uniprot ID: -
• Organism: Pasteurella multocida strain 171865
• Function: type IV pilus biogenesis and function (predicted from homology) DNA binding and uptake

Genomic Context

Location: 2061202..2072587

Locus tag	Gene name	Coordinates (strand)	Size (bp)	Protein ID	Product	Description
ACN6IU_RS10480	-	2061394..2062245 (-)	852	WP_005751072.1	thymidylate synthase	-
ACN6IU_RS10485	lgt	2062251..2063054 (-)	804	WP_005751073.1	prolipoprotein diacylglyceryl transferase	-
ACN6IU_RS10490	-	2063064..2063858 (-)	795	WP_005722415.1	sulfite exporter TauE/SafE family protein	-
ACN6IU_RS10495	rppH	2063861..2064457 (-)	597	WP_005722417.1	RNA pyrophosphohydrolase	-
ACN6IU_RS10500	ampD	2065043..2065609 (-)	567	WP_014326302.1	1,6-anhydro-N-acetylmuramyl-L-alanine amidase AmpD	-
ACN6IU_RS10505	pilA	2065768..2066202 (+)	435	WP_005741096.1	prepilin peptidase-dependent pilin	Machinery gene
ACN6IU_RS10510	pilB	2066202..2067587 (+)	1386	WP_016504529.1	GspE/PulE family protein	Machinery gene
ACN6IU_RS10515	-	2067587..2068798 (+)	1212	WP_014326300.1	type II secretion system F family protein	-
ACN6IU_RS10520	-	2068798..2069520 (+)	723	WP_014326299.1	prepilin peptidase	-
ACN6IU_RS10525	coaE	2069535..2070155 (+)	621	WP_014326298.1	dephospho-CoA kinase	-
ACN6IU_RS10530	yacG	2070166..2070369 (+)	204	WP_005719879.1	DNA gyrase inhibitor YacG	-
ACN6IU_RS10535	-	2070369..2070638 (+)	270	WP_005722568.1	GNAT family N-acetyltransferase	-
ACN6IU_RS10540	-	2071087..2072460 (+)	1374	WP_014326297.1	alanine/glycine:cation symporter family protein	-

Sequence

Protein

• Length: 461 a.a.
• Molecular weight: 52173.85 Da
• Isoelectric Point: 6.5002

>NTDB_id=1106199 ACN6IU_RS10510 WP_016504529.1 2066202..2067587(+) (pilB) [Pasteurella multocida strain 171865]
MFHENPLCTVSAMTGEVFEIQQALWQKNQQQQRFLLRYFALPLQEDEKQLWLGVDSLANLSACDAFSFLTNKHIEPVLLS
TNELKRALQQLSPPKLQVEENIPTVYQVAQLDSDSATDEPLIQLLDQVFERALQLQASDIHFEPQQHSLQVRFRIDGVLQ
VQPSFSGILANRLISRLKLLAKLDISETRLPQDGCFQFKTTFSDTLDFRLSTLPTHLGEKAVLRLQRNKPIHLSFAELGM
TADQQHRFQHALSQPQGLILVTGPTGSGKSISLYSALAWLNSIEKHILTAEDPIEIVLTGIIQTQVNTQIGLDFKRLLRT
FLRQDPDIIMLGEIRDEESALIALRAAQTGHLVLSTLHTNDAPSALTRLQQLGVPLHEIEQSLLLVVAQRLLRRRCDCRV
ETQSACRCYQGYKGRIGVYQFLQKDKTGCFVTDFSSLRESAKEKVRLGMTDDNEVQRVLGE

Nucleotide

• Length: 1386 bp

>NTDB_id=1106199 ACN6IU_RS10510 WP_016504529.1 2066202..2067587(+) (pilB) [Pasteurella multocida strain 171865]
ATGTTTCATGAGAATCCTTTGTGTACTGTTAGCGCAATGACAGGAGAGGTATTTGAAATCCAACAGGCGCTATGGCAAAA
AAATCAGCAACAACAACGTTTTTTATTACGTTATTTTGCCTTGCCTTTACAAGAAGATGAAAAACAGTTGTGGCTTGGTG
TGGATTCGTTAGCGAATTTATCAGCATGTGATGCCTTTTCTTTTTTAACGAATAAACACATTGAGCCTGTTTTATTATCA
ACAAATGAACTGAAACGGGCATTGCAGCAACTTTCCCCACCAAAATTACAGGTGGAAGAAAACATACCCACGGTTTATCA
AGTCGCGCAATTAGATTCTGATTCCGCGACGGATGAACCTTTGATTCAATTACTTGATCAGGTTTTTGAACGTGCATTAC
AGCTACAGGCTTCTGACATTCATTTTGAACCACAACAGCATAGCTTACAAGTGCGTTTTAGAATCGATGGTGTATTACAA
GTCCAGCCTTCCTTTTCAGGGATATTGGCTAATCGCCTGATTTCTCGTCTTAAATTATTAGCAAAATTAGATATTAGTGA
AACACGCTTGCCTCAGGACGGTTGTTTCCAATTTAAAACGACATTTTCTGATACATTAGATTTTCGACTTTCTACGTTGC
CGACGCATTTAGGCGAAAAAGCGGTATTACGTTTGCAGCGAAATAAGCCTATTCATCTCAGTTTTGCTGAGTTGGGGATG
ACCGCAGACCAACAACACAGGTTCCAACACGCGTTAAGTCAACCTCAAGGACTGATTTTAGTAACGGGACCAACAGGGAG
TGGAAAAAGCATTTCGCTTTATAGTGCGCTAGCTTGGCTCAACAGCATAGAAAAGCATATTTTAACAGCAGAAGATCCGA
TTGAAATTGTGTTGACTGGGATAATCCAAACGCAAGTAAATACGCAAATAGGACTGGATTTCAAGCGCTTATTACGCACT
TTTTTACGTCAAGATCCAGACATTATTATGTTAGGGGAAATTCGTGATGAAGAAAGCGCGTTAATCGCATTACGTGCGGC
TCAAACAGGGCATTTAGTCCTCTCTACCTTGCATACGAATGATGCCCCCTCTGCCCTTACTCGCTTACAGCAATTAGGGG
TGCCTTTGCATGAAATTGAACAAAGCCTATTACTTGTTGTGGCGCAGCGTTTATTGCGTCGTCGTTGTGATTGCCGTGTA
GAGACACAATCGGCGTGTCGTTGCTATCAGGGGTATAAAGGGAGGATTGGGGTATATCAATTTTTACAAAAAGATAAAAC
AGGGTGTTTTGTCACGGATTTTAGTAGTTTACGCGAGAGTGCGAAGGAAAAAGTTCGTTTAGGCATGACCGATGACAATG
AAGTACAACGTGTATTAGGTGAATGA

Domains

Predicted by InterproScan.

• Type II secretion system (T2SS), protein E, N-terminal domain (MshEN) (19-93)

• Type II/IV secretion system protein (T2SSE) (128-394)

Similar proteins

Only experimentally validated proteins are listed.

Protein	Organism	Identities (%)	Coverage (%)	Ha-value
pilB	Haemophilus influenzae 86-028NP	68.212	98.265	0.67
pilB	Haemophilus influenzae Rd KW20	67.55	98.265	0.664
pilB	Glaesserella parasuis strain SC1401	58.811	98.482	0.579
pilB	Acinetobacter baumannii D1279779	39.757	100	0.425
pilB	Vibrio campbellii strain DS40M4	39.419	100	0.412
pilB	Acinetobacter baylyi ADP1	37.876	100	0.41
pilB	Vibrio parahaemolyticus RIMD 2210633	39.293	100	0.41
pilB	Vibrio cholerae strain A1552	38.272	100	0.403
pilB	Legionella pneumophila strain ERS1305867	37.219	100	0.395