Detailed information    

insolico Bioinformatically predicted

Overview

Name   pilQ   Type   Machinery gene
Locus tag   ACNYC6_RS11490 Genome accession   NZ_CP183416
Coordinates   2571589..2573658 (-) Length   689 a.a.
NCBI ID   WP_419712399.1    Uniprot ID   -
Organism   Pseudomonas sp. NFX224     
Function   assembly of type IV pilus (predicted from homology)   
DNA binding and uptake

Genomic Context

Location: 2566589..2578658
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  ACNYC6_RS11475 (ACNYC6_11475) - 2568208..2569803 (-) 1596 WP_419712397.1 AAA family ATPase -
  ACNYC6_RS11480 (ACNYC6_11480) aroB 2569814..2570914 (-) 1101 WP_419712398.1 3-dehydroquinate synthase -
  ACNYC6_RS11485 (ACNYC6_11485) aroK 2571066..2571584 (-) 519 WP_047536474.1 shikimate kinase AroK -
  ACNYC6_RS11490 (ACNYC6_11490) pilQ 2571589..2573658 (-) 2070 WP_419712399.1 type IV pilus secretin PilQ Machinery gene
  ACNYC6_RS11495 (ACNYC6_11495) - 2573672..2574199 (-) 528 WP_419712400.1 pilus assembly protein PilP -
  ACNYC6_RS11500 (ACNYC6_11500) pilO 2574196..2574819 (-) 624 WP_419712401.1 type 4a pilus biogenesis protein PilO -
  ACNYC6_RS11505 (ACNYC6_11505) - 2574816..2575382 (-) 567 WP_419712402.1 PilN domain-containing protein -
  ACNYC6_RS11510 (ACNYC6_11510) comM 2575382..2576443 (-) 1062 WP_419712403.1 pilus assembly protein PilM Machinery gene

Sequence

Protein


Download         Length: 689 a.a.        Molecular weight: 74693.91 Da        Isoelectric Point: 5.4172

>NTDB_id=1105648 ACNYC6_RS11490 WP_419712399.1 2571589..2573658(-) (pilQ) [Pseudomonas sp. NFX224]
MNRIFSTLGVSLWIALLSPMVQAANLKALDVAALPGDRVELKLAFDEPPPAPHGYTTESPARIALDLPGVVSQLASKTRD
LGGGNARSATVVEAKDRTRLIINLTRLTPYDTRIEGNSLFVVVGQGGNNPAPRRAAVAAPAPARAVAPVAKAIRSVDFQR
GTQGEGNVVIDLTDPSIAPDIQEREGKIILGFARTRLPEPLRVRLDVKDFATPVQFVNASATGDRATITIEPSGAFDYST
YQTDNKLTVSIRPMTADDLQKRNADRYAYSGEKLSLNFQDIDVRSVLQLIADFTNLNLVASDTVQGGITLRLQNVPWDQA
LDLVLKTKGLDKRKIGNVLLVAPADEIAARERQELESQKQIADLAPLRRELLQVNYAKAADIAKLFQSVTSAEAKVDERG
SITVDERTNNIIAYQTQDRLDELRRIVAQLDIPVRQVMIEARIVEANVDYDKSLGVRWGGSIQNKGNWNTSGVSNGANAS
STIGTPGSTSTNSPFVDLGAVNNTSGIGIAFITDNVLLDLELTAMEKTGNGEIVSQPKVVTSDKETAKILKGTEIPYQEA
SSSGATSVSFKEASLSLEVTPQITPDNRIIMEVKVTKDEPDYLNKVQDVPPIKKNEVNAKVLVNDGETIVIGGVFSNTQS
KVVDKVPFLGDVPYLGRLFRRDVVSEKKSELLVFLTPRIMNNQAIAVSR

Nucleotide


Download         Length: 2070 bp        

>NTDB_id=1105648 ACNYC6_RS11490 WP_419712399.1 2571589..2573658(-) (pilQ) [Pseudomonas sp. NFX224]
ATGAACAGGATTTTCTCCACCCTCGGAGTGTCGCTATGGATAGCGCTGCTGTCGCCGATGGTACAAGCGGCCAACCTCAA
GGCGCTGGATGTCGCAGCGCTGCCGGGCGACCGCGTCGAGTTGAAGTTGGCGTTCGATGAGCCTCCCCCGGCGCCTCACG
GGTACACCACCGAATCGCCTGCGCGGATTGCGCTGGACTTGCCCGGTGTCGTCAGTCAGCTGGCGAGCAAGACCCGCGAT
CTGGGCGGCGGCAATGCCCGCAGCGCAACGGTGGTGGAAGCCAAGGACCGGACACGGCTGATCATCAACCTCACTCGATT
GACCCCGTATGACACCCGGATCGAAGGCAACAGCCTGTTTGTGGTGGTCGGCCAGGGAGGCAACAACCCGGCGCCGAGGC
GGGCCGCTGTCGCCGCTCCGGCTCCGGCGCGCGCCGTTGCGCCAGTGGCCAAGGCCATTCGCTCGGTAGATTTCCAGCGC
GGCACCCAGGGCGAAGGCAATGTAGTGATCGACCTGACGGATCCGTCCATCGCGCCAGACATTCAGGAGCGCGAGGGCAA
GATCATTCTCGGTTTCGCCAGAACCCGATTGCCTGAGCCGTTGCGTGTGCGCCTGGACGTCAAGGACTTCGCCACGCCCG
TACAGTTCGTCAATGCCAGCGCTACAGGCGACAGGGCCACTATTACCATCGAGCCCAGCGGTGCCTTCGATTATTCGACC
TACCAGACCGACAACAAGCTGACCGTCAGCATCCGCCCGATGACTGCCGACGACTTGCAAAAGCGCAATGCCGACCGCTA
CGCCTACAGCGGTGAAAAGCTCTCGTTGAATTTTCAGGACATCGATGTGCGCTCGGTGCTGCAATTGATCGCCGACTTCA
CCAACCTCAATCTGGTGGCCAGCGACACGGTGCAAGGCGGCATTACCCTGCGTTTACAAAACGTGCCGTGGGATCAGGCG
CTGGATCTGGTGCTGAAAACCAAGGGGCTGGACAAGCGCAAGATCGGCAACGTTCTGTTGGTGGCGCCGGCCGATGAAAT
CGCTGCCCGTGAGCGCCAGGAACTGGAGTCGCAGAAGCAAATCGCTGATCTGGCCCCCCTGCGGCGGGAGTTGCTACAGG
TCAACTACGCCAAGGCAGCCGATATCGCCAAGCTGTTCCAGTCGGTGACCAGCGCTGAAGCGAAAGTCGACGAACGGGGT
TCGATCACCGTCGATGAGCGTACCAACAACATCATTGCCTACCAGACTCAGGATCGGCTCGACGAATTGCGGCGGATCGT
GGCGCAGCTGGATATCCCGGTACGGCAGGTGATGATCGAGGCGCGGATCGTCGAAGCCAACGTTGATTACGACAAGAGCC
TGGGTGTGCGCTGGGGCGGTTCGATCCAGAATAAGGGCAACTGGAATACATCAGGCGTCAGCAATGGTGCAAACGCATCG
TCGACCATCGGCACGCCAGGTAGCACCAGCACCAATTCGCCGTTCGTCGACTTGGGAGCCGTCAACAACACCTCTGGAAT
CGGCATCGCGTTCATCACCGACAACGTCTTGCTCGACCTTGAACTGACGGCCATGGAGAAAACCGGCAACGGCGAAATCG
TCTCGCAACCCAAAGTGGTTACGTCCGACAAGGAAACCGCGAAAATCCTCAAGGGAACCGAGATTCCCTATCAGGAAGCG
AGCTCCAGCGGCGCGACTTCCGTGTCTTTCAAGGAAGCCTCGCTGTCGCTGGAAGTGACGCCGCAAATCACCCCGGACAA
CCGGATCATCATGGAGGTCAAGGTCACCAAGGACGAGCCTGACTACCTGAACAAGGTCCAGGATGTGCCGCCGATCAAGA
AAAACGAGGTCAACGCCAAGGTCCTGGTCAACGATGGCGAGACCATCGTCATTGGTGGTGTTTTCTCAAATACTCAAAGC
AAGGTTGTAGATAAGGTGCCATTTCTTGGCGATGTGCCGTATCTTGGCCGCCTTTTCCGGCGTGACGTGGTTTCGGAGAA
AAAATCCGAGCTGTTGGTGTTTCTCACTCCGCGTATCATGAATAACCAGGCGATTGCTGTGAGTCGTTGA

Domains

Predicted by InterproScan.

Secretin and TonB N terminus short domain (STN)

(297-343)

Bacterial type II/III secretion system short domain (Secretin_N)

(371-436)

AMIN domain (AMIN)

(38-121)

Bacterial type II and III secretion system protein (Secretin)

(524-680)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  pilQ Pseudomonas aeruginosa PAK

72.841

100

0.759
  comQ Acinetobacter baylyi ADP1

41.295

100

0.417
  pilQ Acinetobacter baumannii strain A118

38.275

100

0.412
  pilQ Acinetobacter baumannii D1279779

38.275

100

0.412
  pilQ Legionella pneumophila strain ERS1305867

38.69

97.533

0.377
  pilQ Legionella pneumophila strain Lp02

38.69

97.533

0.377

Multiple sequence alignment