Detailed information    

insolico Bioinformatically predicted

Overview

Name   pilB   Type   Machinery gene
Locus tag   ACAM37_RS09490 Genome accession   NZ_AP031595
Coordinates   2149590..2151296 (-) Length   568 a.a.
NCBI ID   WP_369626091.1    Uniprot ID   -
Organism   Moraxella sp. K23     
Function   power the assembly of type IV pilus (predicted from homology)   
DNA binding and uptake

Genomic Context

Location: 2144590..2156296
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  ACAM37_RS09470 (MOXK23_18390) - 2145312..2146718 (+) 1407 WP_065253798.1 nicotinate phosphoribosyltransferase -
  ACAM37_RS09475 (MOXK23_18400) coaE 2146776..2147432 (-) 657 WP_239743400.1 dephospho-CoA kinase -
  ACAM37_RS09480 (MOXK23_18410) pilD 2147429..2148304 (-) 876 WP_369626090.1 A24 family peptidase Machinery gene
  ACAM37_RS09485 (MOXK23_18420) pilC 2148310..2149533 (-) 1224 WP_128680796.1 type II secretion system F family protein Machinery gene
  ACAM37_RS09490 (MOXK23_18430) pilB 2149590..2151296 (-) 1707 WP_369626091.1 type IV-A pilus assembly ATPase PilB Machinery gene
  ACAM37_RS09495 (MOXK23_18440) tpiA 2151715..2152554 (+) 840 WP_369626092.1 triose-phosphate isomerase -
  ACAM37_RS09500 (MOXK23_18450) secG 2152645..2152965 (+) 321 WP_353551321.1 preprotein translocase subunit SecG -
  ACAM37_RS09515 (MOXK23_18460) rimP 2153529..2154038 (+) 510 WP_369626093.1 ribosome maturation factor RimP -
  ACAM37_RS09520 (MOXK23_18470) nusA 2154156..2155637 (+) 1482 WP_007115082.1 transcription termination factor NusA -

Sequence

Protein


Download         Length: 568 a.a.        Molecular weight: 62679.12 Da        Isoelectric Point: 5.3368

>NTDB_id=110563 ACAM37_RS09490 WP_369626091.1 2149590..2151296(-) (pilB) [Moraxella sp. K23]
MAASLPGFGGIASRLVHEGLISEASMQKVLAESQQQKTNLIAYVVDNKLAQPDAVAWVISREFGDPLIDLDAINVNYIPK
EAVDEKIIREFNILPIFQRGSRLFVAMSDPTRVDALDALRFGTRFTVETVVAEHHKIKSLIEKIFTTTNLADFDDMEAVE
FDETRADEDNSATSHLDINDEAPVVKFINGMLIKAITMGASDLHFEPYEKSYRVRFRIDGVLQKMANPPTQLAGKIAARL
KVMSQMDISERRVPQDGRIKLKISKDKAIDFRVNSLPTLFGEKIVLRILDPSSAMLGIDALGYEPDQKQLFMDALAKPQG
MLLITGPTGSGKTVSLYTGLNILNTVETNISTAEDPVEINLEGINQVNVNNKVGLTFASALKSFLRQDPDIVMVGEIRDL
ETAEIAVKAAQTGHMVLSTLHTNSAPETLTRLRNMGVASFNIATSVNLVIAQRLARRLCTACKKPTNVPKNSLLEMGFTE
EDLADTSNVIYEPVGCSECRDGYKGRVGIYEVMKVTPEIARIIMEDGNAIEIKDAALKQGFRDLRRSGILKVLKGTTSIQ
EMYRVTSE

Nucleotide


Download         Length: 1707 bp        

>NTDB_id=110563 ACAM37_RS09490 WP_369626091.1 2149590..2151296(-) (pilB) [Moraxella sp. K23]
ATGGCAGCATCACTTCCTGGTTTTGGTGGCATTGCGTCACGATTAGTGCATGAAGGACTAATTAGCGAAGCGTCAATGCA
AAAAGTATTGGCAGAATCGCAGCAACAAAAAACAAATTTAATCGCCTATGTGGTTGACAATAAATTGGCGCAACCTGACG
CGGTAGCGTGGGTAATTTCGCGAGAATTTGGCGACCCACTCATTGATTTGGATGCCATCAATGTCAACTATATTCCAAAA
GAAGCGGTTGATGAAAAAATTATTCGCGAATTTAATATTCTGCCGATTTTTCAACGAGGTAGCCGCTTATTTGTTGCTAT
GAGTGACCCAACAAGGGTAGATGCTTTAGATGCACTGCGGTTTGGTACAAGGTTTACCGTAGAAACAGTGGTTGCCGAAC
ACCACAAAATCAAATCACTCATCGAAAAAATCTTTACTACCACCAATTTAGCTGATTTTGATGATATGGAAGCGGTGGAG
TTTGATGAAACCCGTGCCGATGAAGACAATAGTGCCACCAGTCATTTAGATATCAATGATGAAGCGCCTGTCGTTAAGTT
CATTAATGGGATGCTCATCAAAGCCATTACCATGGGCGCTTCAGACTTACATTTTGAACCCTATGAAAAATCTTATCGCG
TGCGTTTTCGTATTGATGGCGTGTTACAAAAAATGGCAAATCCACCGACGCAGCTAGCGGGTAAAATTGCTGCCCGGCTG
AAAGTCATGTCACAAATGGACATCTCTGAACGCCGCGTGCCGCAAGATGGTCGTATCAAATTAAAAATTTCCAAGGATAA
GGCGATTGATTTTCGTGTCAACTCACTGCCAACCTTATTTGGTGAAAAAATTGTACTGCGTATTTTAGATCCATCATCAG
CGATGCTAGGCATTGATGCACTCGGCTATGAGCCGGACCAAAAGCAGCTATTTATGGATGCGCTGGCAAAACCGCAAGGG
ATGTTATTGATTACCGGCCCTACGGGTTCGGGTAAAACCGTCTCCTTGTACACGGGTCTTAATATCTTAAACACAGTTGA
GACCAATATTTCCACCGCAGAAGATCCCGTTGAAATTAACCTTGAAGGCATCAATCAGGTCAACGTGAATAATAAAGTTG
GCTTGACCTTTGCCAGTGCGCTCAAATCATTCTTGCGTCAAGACCCTGATATTGTGATGGTCGGTGAGATTCGTGACTTG
GAAACGGCGGAAATTGCTGTCAAAGCTGCCCAAACAGGGCATATGGTACTGTCAACCTTGCATACCAACTCAGCGCCAGA
AACCTTAACGCGTCTGCGGAATATGGGTGTGGCATCTTTTAACATTGCCACCTCAGTCAATTTGGTAATCGCACAGCGCT
TAGCACGTCGCCTTTGTACAGCTTGTAAAAAACCGACCAATGTGCCAAAAAATAGCTTGCTTGAAATGGGCTTTACGGAA
GAAGATTTGGCAGATACCAGCAATGTCATTTATGAGCCCGTTGGCTGCTCTGAATGCCGCGATGGCTATAAAGGACGTGT
TGGTATTTATGAAGTGATGAAAGTAACACCAGAGATTGCCCGAATTATTATGGAAGACGGCAATGCTATTGAGATTAAAG
ACGCTGCCTTAAAGCAAGGGTTTAGGGATTTAAGGCGATCTGGCATTTTAAAGGTTCTAAAAGGCACGACCAGTATCCAA
GAGATGTATCGTGTTACTAGCGAATAG

Domains

Predicted by InterproScan.

Type II secretion system (T2SS), protein E, N-terminal domain (MshEN)

(15-145)

Type II/IV secretion system protein (T2SSE)

(191-457)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  pilB Acinetobacter baumannii D1279779

68.021

99.648

0.678
  pilB Acinetobacter baylyi ADP1

65.603

99.296

0.651
  pilB Legionella pneumophila strain ERS1305867

50.883

99.648

0.507
  pilF Neisseria gonorrhoeae MS11

49.374

98.415

0.486
  pilB Vibrio cholerae strain A1552

48.658

98.415

0.479
  pilB Vibrio parahaemolyticus RIMD 2210633

49.18

96.655

0.475
  pilB Vibrio campbellii strain DS40M4

46.607

98.592

0.46
  pilB Deinococcus radiodurans R1 = ATCC 13939 = DSM 20539

37.856

100

0.398
  pilF Thermus thermophilus HB27

36.649

100

0.37

Multiple sequence alignment