Detailed information    

insolico Bioinformatically predicted

Overview

Name   pilB   Type   Machinery gene
Locus tag   ACN3VN_RS10825 Genome accession   NZ_CP183396
Coordinates   2346849..2348582 (+) Length   577 a.a.
NCBI ID   WP_058569268.1    Uniprot ID   -
Organism   Xylella fastidiosa strain CFBP8074     
Function   power the assembly of type IV pilus (predicted from homology)   
DNA binding and uptake

Genomic Context

Location: 2341849..2353582
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  ACN3VN_RS10800 (ACN3VN_10800) pilC 2342174..2343376 (-) 1203 WP_011098309.1 type II secretion system F family protein Machinery gene
  ACN3VN_RS10805 (ACN3VN_10805) - 2343817..2344266 (+) 450 WP_058569266.1 pilin -
  ACN3VN_RS10810 (ACN3VN_10810) - 2344531..2344734 (-) 204 WP_419653900.1 hypothetical protein -
  ACN3VN_RS10815 (ACN3VN_10815) - 2345239..2345367 (-) 129 WP_267885865.1 hypothetical protein -
  ACN3VN_RS10820 (ACN3VN_10820) - 2345568..2346008 (+) 441 WP_058569267.1 pilin -
  ACN3VN_RS10825 (ACN3VN_10825) pilB 2346849..2348582 (+) 1734 WP_058569268.1 type IV-A pilus assembly ATPase PilB Machinery gene
  ACN3VN_RS10830 (ACN3VN_10830) pilR 2348676..2350088 (-) 1413 WP_058569269.1 sigma-54-dependent transcriptional regulator Regulator
  ACN3VN_RS10835 (ACN3VN_10835) - 2350274..2351884 (-) 1611 WP_058569270.1 sensor histidine kinase -
  ACN3VN_RS10840 (ACN3VN_10840) sucC 2352133..2353296 (+) 1164 WP_046418841.1 ADP-forming succinate--CoA ligase subunit beta -

Sequence

Protein


Download         Length: 577 a.a.        Molecular weight: 63049.11 Da        Isoelectric Point: 5.9154

>NTDB_id=1105523 ACN3VN_RS10825 WP_058569268.1 2346849..2348582(+) (pilB) [Xylella fastidiosa strain CFBP8074]
MNTPHSANLVGITGIARRLVQDGSIEEATARTAQNQAMAAKVPLPQWLIDKKLVSAAKLAAANALEFGMPLLDVSAFDHN
HNAMNLVDEELLQKHRVLPLFKRGNRLFVGISNPTQTQALDDIKFHTNLVVEPILVDEDQIRRTLEQWQSRSGALSSTLE
EEDNGIEGLDIGGGDDDGASSDTGIDAKSDDTPVVKFVNKVLIDAIRRGASDIHFEPYEEDYRVRFRIDGLLKAVAKAPN
KLNQRIAARLKVMSQLDIAEKRVPQDGRIKLNLSKTKQVDFRVSTLPTLFGEKVVLRILDGSAAKLGIDKLGYEADQQTL
FMEAIHKPYGMVLVTGPTGSGKTVSLYTALGILNDETRNISTAEDPVEIRLPGVNQVQQNNKRGMTFAAALRSFLRQDPD
VIMVGEIRDLETAEIAIKAAQTGHMVLSTLHTNDAPQTIARLMNMGIAPYNITSSVTLVIAQRLARRLCNKCKRPVQLPT
NALLAEGFTQEQIDAGLELYEPVGCDECTEGYKGRTGIYQVMPITEGISSIILAGGNALQIAEEAQRIGINDLRQSALLK
VAQGITGLAEINRVTKD

Nucleotide


Download         Length: 1734 bp        

>NTDB_id=1105523 ACN3VN_RS10825 WP_058569268.1 2346849..2348582(+) (pilB) [Xylella fastidiosa strain CFBP8074]
ATGAATACACCTCATTCAGCCAATCTTGTCGGCATCACCGGTATTGCCCGTCGCCTGGTCCAGGATGGCAGCATTGAGGA
AGCCACTGCACGCACTGCTCAAAATCAGGCAATGGCCGCAAAAGTGCCTTTACCACAGTGGTTGATCGATAAAAAACTAG
TGAGTGCAGCAAAATTGGCCGCGGCCAATGCACTTGAGTTCGGGATGCCGCTACTGGATGTGTCCGCATTCGACCACAAT
CACAATGCAATGAACTTGGTCGACGAAGAATTACTGCAAAAGCATCGGGTGTTGCCATTATTTAAACGCGGTAATCGACT
GTTCGTCGGGATCAGCAATCCAACCCAAACACAGGCCCTGGACGACATCAAATTCCACACCAATCTGGTCGTGGAACCGA
TTCTGGTCGACGAAGACCAGATACGACGCACACTTGAACAATGGCAATCTAGGAGCGGCGCGCTCAGCTCCACATTGGAG
GAAGAGGATAACGGCATTGAAGGGCTGGATATCGGCGGCGGAGACGACGACGGGGCCAGTAGCGATACAGGGATTGATGC
CAAGAGCGATGACACACCTGTCGTCAAATTCGTCAACAAAGTGCTGATTGATGCCATCCGACGCGGCGCATCAGACATCC
ACTTTGAACCATACGAAGAAGATTACCGCGTCCGTTTCCGGATTGATGGTCTGCTGAAGGCTGTGGCAAAAGCACCGAAC
AAGTTGAATCAGCGTATCGCCGCACGCTTGAAAGTCATGTCGCAACTGGATATCGCGGAAAAACGCGTCCCTCAAGACGG
ACGTATCAAGCTTAATCTGTCGAAAACCAAGCAAGTCGATTTCCGCGTCAGCACATTGCCAACGCTGTTCGGCGAGAAAG
TGGTGCTGCGTATCTTGGATGGCAGCGCGGCAAAGCTGGGTATCGACAAACTCGGTTACGAAGCTGACCAACAAACGCTT
TTTATGGAAGCAATCCACAAACCCTATGGAATGGTATTGGTCACTGGTCCAACCGGTTCGGGCAAGACAGTGTCACTGTA
CACTGCACTAGGCATCCTCAATGACGAAACACGCAATATCTCCACAGCCGAAGATCCTGTAGAAATCCGGCTGCCGGGCG
TCAATCAAGTACAACAAAACAACAAACGCGGTATGACGTTTGCCGCAGCATTGCGTTCGTTTCTGCGCCAAGACCCGGAT
GTCATCATGGTCGGCGAAATCCGAGACCTGGAAACCGCTGAAATCGCCATCAAAGCCGCACAGACCGGCCACATGGTGCT
CTCCACACTACATACCAACGATGCACCGCAAACCATCGCACGCCTCATGAACATGGGGATTGCACCATACAACATCACTT
CATCCGTGACCTTAGTGATTGCACAGCGATTGGCACGGAGATTGTGCAATAAATGCAAGCGCCCGGTTCAATTGCCGACA
AACGCATTACTGGCCGAAGGATTCACTCAAGAACAAATTGATGCGGGGCTAGAATTATACGAGCCTGTCGGTTGCGACGA
ATGCACCGAAGGCTACAAAGGCCGCACAGGTATTTATCAAGTGATGCCAATTACCGAAGGGATTTCCTCGATCATTTTAG
CCGGCGGTAATGCCTTACAAATCGCCGAAGAGGCACAGCGCATCGGCATCAACGACCTACGCCAATCGGCGCTACTCAAA
GTCGCTCAGGGCATCACCGGGCTGGCTGAAATTAATCGAGTCACCAAAGATTAG

Domains

Predicted by InterproScan.

Type II/IV secretion system protein (T2SSE)

(200-467)

Type II secretion system (T2SS), protein E, N-terminal domain (MshEN)

(48-152)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  pilB Acinetobacter baylyi ADP1

57.143

98.267

0.562
  pilB Acinetobacter baumannii D1279779

56.437

98.267

0.555
  pilB Legionella pneumophila strain ERS1305867

52.113

98.44

0.513
  pilF Neisseria gonorrhoeae MS11

50.439

98.614

0.497
  pilB Vibrio cholerae strain A1552

49.908

94.107

0.47
  pilB Vibrio parahaemolyticus RIMD 2210633

47.679

97.054

0.463
  pilB Vibrio campbellii strain DS40M4

46.303

98.44

0.456
  pilB Deinococcus radiodurans R1 = ATCC 13939 = DSM 20539

38.993

92.894

0.362
  pilF Thermus thermophilus HB27

40.077

89.948

0.36

Multiple sequence alignment