Detailed information    

insolico Bioinformatically predicted

Overview

Name   pilB   Type   Machinery gene
Locus tag   ACAM39_RS10235 Genome accession   NZ_AP031590
Coordinates   2263767..2265473 (-) Length   568 a.a.
NCBI ID   WP_369627607.1    Uniprot ID   -
Organism   Moraxella sp. K02     
Function   power the assembly of type IV pilus (predicted from homology)   
DNA binding and uptake

Genomic Context

Location: 2258767..2270473
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  ACAM39_RS10215 (MOXK02_19980) - 2259489..2260895 (+) 1407 WP_369627603.1 nicotinate phosphoribosyltransferase -
  ACAM39_RS10220 (MOXK02_19990) coaE 2260953..2261609 (-) 657 WP_369627604.1 dephospho-CoA kinase -
  ACAM39_RS10225 (MOXK02_20000) pilD 2261606..2262481 (-) 876 WP_369627605.1 A24 family peptidase Machinery gene
  ACAM39_RS10230 (MOXK02_20010) pilC 2262487..2263710 (-) 1224 WP_369627606.1 type II secretion system F family protein Machinery gene
  ACAM39_RS10235 (MOXK02_20020) pilB 2263767..2265473 (-) 1707 WP_369627607.1 type IV-A pilus assembly ATPase PilB Machinery gene
  ACAM39_RS10240 (MOXK02_20030) tpiA 2265931..2266785 (+) 855 WP_369627608.1 triose-phosphate isomerase -
  ACAM39_RS10245 (MOXK02_20040) secG 2266877..2267197 (+) 321 WP_036594931.1 preprotein translocase subunit SecG -
  ACAM39_RS10260 (MOXK02_20050) rimP 2267761..2268270 (+) 510 WP_007115081.1 ribosome maturation factor RimP -
  ACAM39_RS10265 (MOXK02_20060) nusA 2268388..2269869 (+) 1482 WP_065264333.1 transcription termination factor NusA -

Sequence

Protein


Download         Length: 568 a.a.        Molecular weight: 62631.10 Da        Isoelectric Point: 5.3368

>NTDB_id=110545 ACAM39_RS10235 WP_369627607.1 2263767..2265473(-) (pilB) [Moraxella sp. K02]
MAASLPGFGGIASRLVHEGLISEAAMQKVLAESQQQKTNLIAYVVENKLAQADAVAWVISREFGDPLIDLDAINVNYIPK
EAVDEKIIREFNILPIFQRGSRLFVAMSDPTRVDALDALRFGTRFTVETVVAEHHKIKSLIEKIFATSNLADFDDMEAVE
FDENRADDDNSATSHLDVNDEAPVVKFVNGMLIKAITMGASDLHFEPYEKSYRVRFRIDGVLQKMANPPTQLAGKIAARL
KVMSQMDISERRVPQDGRIKLKISKDKAIDFRVNSLPTLFGEKIVLRILDPSSAMLGIDALGYEPDQKQLFMDALAKPQG
MLLITGPTGSGKTVSLYTGLNILNTVETNISTAEDPVEINLEGINQVNVNNKVGLTFASALKSFLRQDPDIVMVGEIRDL
ETAEIAVKAAQTGHMVLSTLHTNSAPETLTRLRNMGVASFNIATSVNLVIAQRLARRLCKSCKKPTDVPKNSLLEMGFME
EDLANPSNVIYEPVGCAECRDGYKGRVGIYEVMKVTPEIARIIMEDGNAIEIKDAALKQGFRDLRRSGILKVLQGTTSIQ
EMYRVTSE

Nucleotide


Download         Length: 1707 bp        

>NTDB_id=110545 ACAM39_RS10235 WP_369627607.1 2263767..2265473(-) (pilB) [Moraxella sp. K02]
ATGGCAGCATCCCTTCCTGGTTTTGGTGGCATTGCCTCGCGATTAGTGCATGAAGGACTAATTAGCGAAGCGGCAATGCA
AAAAGTATTGGCAGAATCGCAGCAGCAAAAAACAAATTTAATCGCCTATGTGGTTGAAAATAAGCTCGCGCAAGCTGACG
CAGTAGCGTGGGTAATTTCGCGAGAATTTGGCGATCCCCTCATTGATTTGGATGCCATCAATGTCAACTATATTCCAAAA
GAAGCGGTTGATGAAAAAATTATTCGCGAATTTAATATTCTGCCGATTTTCCAACGAGGTAGCCGCTTATTTGTTGCCAT
GAGTGACCCGACGCGGGTGGATGCTTTAGATGCACTGCGATTTGGTACAAGATTTACTGTAGAAACAGTGGTTGCCGAAC
ACCACAAAATCAAATCACTCATCGAAAAAATCTTTGCTACCAGCAATTTAGCTGATTTTGATGATATGGAAGCGGTGGAG
TTTGATGAAAACCGTGCCGATGACGACAATAGTGCCACCAGTCATTTAGATGTCAATGATGAAGCGCCTGTCGTCAAGTT
CGTTAATGGGATGCTCATCAAAGCCATTACCATGGGCGCCTCAGACTTACACTTTGAACCCTATGAAAAATCTTATCGCG
TGCGTTTTCGTATTGATGGGGTGTTACAAAAAATGGCAAATCCACCGACGCAGCTAGCGGGTAAAATTGCTGCCCGCCTG
AAAGTCATGTCACAAATGGACATCTCTGAACGCCGCGTGCCGCAAGATGGTCGTATCAAATTAAAAATTTCCAAGGATAA
GGCGATTGATTTTCGTGTCAACTCACTGCCAACCTTATTTGGTGAAAAAATCGTACTGCGTATTTTAGACCCATCATCAG
CGATGCTAGGCATTGATGCACTCGGCTATGAGCCGGACCAAAAGCAACTATTTATGGATGCGCTGGCAAAACCGCAAGGG
ATGTTATTGATTACCGGCCCTACTGGTTCGGGTAAAACTGTCTCCTTGTACACGGGTCTTAATATCTTAAACACGGTTGA
GACCAATATTTCCACCGCAGAGGATCCCGTTGAGATTAACCTTGAAGGCATCAATCAGGTCAATGTGAACAATAAAGTTG
GCTTGACCTTTGCCAGTGCGCTCAAATCATTCTTGCGTCAAGACCCTGATATTGTGATGGTCGGTGAGATTCGTGACTTG
GAAACGGCTGAAATTGCTGTCAAAGCTGCCCAAACAGGGCATATGGTGCTGTCAACCTTGCATACCAACTCAGCGCCAGA
AACCTTAACGCGTCTGCGTAATATGGGTGTGGCATCTTTTAACATTGCCACCTCAGTCAATTTGGTAATCGCACAGCGCT
TAGCCCGTCGCCTTTGTAAATCCTGCAAAAAACCGACAGATGTGCCAAAAAATAGCTTGCTTGAAATGGGCTTTATGGAA
GAAGATTTGGCAAATCCCAGCAATGTCATTTATGAGCCCGTTGGCTGCGCTGAATGCCGCGATGGCTATAAAGGACGTGT
TGGTATTTATGAAGTGATGAAAGTAACACCAGAGATTGCCCGAATTATTATGGAAGACGGCAACGCTATTGAGATTAAAG
ACGCGGCTTTAAAGCAAGGGTTTAGGGATTTAAGGCGCTCTGGCATTTTAAAGGTTCTACAAGGCACGACCAGTATCCAA
GAGATGTATCGTGTTACTAGCGAATAG

Domains

Predicted by InterproScan.

Type II secretion system (T2SS), protein E, N-terminal domain (MshEN)

(15-147)

Type II/IV secretion system protein (T2SSE)

(191-457)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  pilB Acinetobacter baumannii D1279779

68.198

99.648

0.68
  pilB Acinetobacter baylyi ADP1

65.724

99.648

0.655
  pilB Legionella pneumophila strain ERS1305867

51.59

99.648

0.514
  pilB Vibrio cholerae strain A1552

49.729

97.359

0.484
  pilF Neisseria gonorrhoeae MS11

49.195

98.415

0.484
  pilB Vibrio parahaemolyticus RIMD 2210633

47.425

99.12

0.47
  pilB Vibrio campbellii strain DS40M4

46.702

98.768

0.461
  pilB Deinococcus radiodurans R1 = ATCC 13939 = DSM 20539

39.854

96.303

0.384
  pilF Thermus thermophilus HB27

36.649

100

0.37

Multiple sequence alignment