Detailed information    

insolico Bioinformatically predicted

Overview

Name   pilB   Type   Machinery gene
Locus tag   ACN08L_RS02570 Genome accession   NZ_CP183285
Coordinates   416495..418180 (-) Length   561 a.a.
NCBI ID   WP_419237151.1    Uniprot ID   -
Organism   Photobacterium leiognathi subsp. mandapamensis strain Sr3.21     
Function   assembly of type IV pilus (predicted from homology)   
DNA binding and uptake

Genomic Context

Location: 411495..423180
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  ACN08L_RS02540 (ACN08L_02535) mutT 412211..412615 (-) 405 WP_305460309.1 8-oxo-dGTP diphosphatase MutT -
  ACN08L_RS02545 (ACN08L_02540) yacG 412620..412841 (-) 222 WP_008987916.1 DNA gyrase inhibitor YacG -
  ACN08L_RS02550 (ACN08L_02545) zapD 412849..413595 (-) 747 WP_042117295.1 cell division protein ZapD -
  ACN08L_RS02555 (ACN08L_02550) coaE 413660..414265 (-) 606 WP_318423279.1 dephospho-CoA kinase -
  ACN08L_RS02560 (ACN08L_02555) pilD 414269..415138 (-) 870 WP_419237150.1 prepilin peptidase Machinery gene
  ACN08L_RS02565 (ACN08L_02560) pilC 415227..416450 (-) 1224 WP_305464597.1 type II secretion system F family protein Machinery gene
  ACN08L_RS02570 (ACN08L_02565) pilB 416495..418180 (-) 1686 WP_419237151.1 type IV-A pilus assembly ATPase PilB Machinery gene
  ACN08L_RS02575 (ACN08L_02570) pilA 418184..418573 (-) 390 WP_045068531.1 pilin Machinery gene
  ACN08L_RS02580 (ACN08L_02575) nadC 418921..419808 (-) 888 WP_419238219.1 carboxylating nicotinate-nucleotide diphosphorylase -
  ACN08L_RS02585 (ACN08L_02580) ampD 420325..420870 (+) 546 WP_042117298.1 1,6-anhydro-N-acetylmuramyl-L-alanine amidase AmpD -
  ACN08L_RS02590 (ACN08L_02585) pdhR 421192..421965 (+) 774 WP_107175991.1 pyruvate dehydrogenase complex transcriptional repressor PdhR -

Sequence

Protein


Download         Length: 561 a.a.        Molecular weight: 62145.65 Da        Isoelectric Point: 5.0204

>NTDB_id=1104990 ACN08L_RS02570 WP_419237151.1 416495..418180(-) (pilB) [Photobacterium leiognathi subsp. mandapamensis strain Sr3.21]
MQTNLASILAQANLISPEQEQQVVEFVTAEGINVPTALTRLGILSDLDLADNLANLFGLQKVDVHHYDYDGCCQSVYQRE
LVLRHRALPLTQDANSLFLGVSDPTNMDAQDEFRFAVGKTVEFLILENKQLESAIRRVYGSDIGETSHCKQVSDADLGEL
VDISDAELDEATDLTQDSAPVTRYINQVLLDAVRRKASDIHFEPYEHNYRIRFRCDGILHQHLTPPASLSRRLSTRLKVM
SKLNIAERRQPQDGRIKLKLSDTLAIDLRVSTLPTLWGEKVVLRILDSSSANLDINILGYNDKQKSDYLTALERPQGMIL
ITGPTGSGKTVSLYTGLNILNTDERNISTAEDPVEINLPGINQVQINQQVGLSFADALRSFLRQDPDIVMVGEIRDLETA
SIAVKAAQTGHLVLSTLHTNSAAETITRLTNMGVEDFNLASSLSLIIAQRLARRLCKSCKHPHPLDEITCQRLNVPMNTT
VYQANNAGCDDCNKGYAGRVGIYEVMVFSREIAEALMANATALQLEDIAVKQGMQRLKDSGIEKLLEGVTSLSELQRVLQ
L

Nucleotide


Download         Length: 1686 bp        

>NTDB_id=1104990 ACN08L_RS02570 WP_419237151.1 416495..418180(-) (pilB) [Photobacterium leiognathi subsp. mandapamensis strain Sr3.21]
ATGCAAACCAATCTCGCTTCTATCTTAGCTCAAGCTAATTTAATTAGCCCAGAGCAAGAACAACAAGTAGTCGAGTTTGT
GACGGCGGAAGGGATTAACGTCCCGACCGCCTTAACCCGTTTAGGGATATTAAGTGATTTAGATTTAGCCGATAACCTAG
CTAATTTATTTGGTTTACAAAAAGTCGATGTTCACCATTATGACTATGATGGTTGTTGTCAAAGCGTATACCAACGTGAA
TTAGTATTGCGCCACCGCGCCCTACCATTAACCCAAGATGCTAACAGTCTATTTTTAGGAGTCAGCGATCCCACAAATAT
GGATGCGCAAGACGAATTTCGTTTTGCAGTGGGTAAAACCGTTGAATTTCTAATTTTAGAAAACAAACAGTTAGAATCCG
CCATTCGTCGTGTATACGGTAGCGATATTGGTGAAACCAGCCATTGTAAACAAGTCAGTGACGCCGATTTAGGTGAGTTG
GTTGATATTAGCGATGCTGAGCTTGATGAAGCGACAGATTTAACCCAAGACAGCGCCCCAGTTACTCGCTACATCAACCA
AGTATTGTTAGATGCCGTACGTCGTAAAGCCTCTGATATTCACTTTGAACCCTACGAGCATAACTACCGTATTCGTTTTC
GCTGTGACGGTATATTGCACCAACATTTAACGCCACCAGCGAGTTTGTCACGTCGTTTATCAACCCGTTTAAAGGTGATG
TCAAAGCTCAACATTGCTGAGCGTCGCCAACCGCAAGACGGACGTATTAAGTTAAAACTATCAGATACCTTAGCAATAGA
TTTACGTGTCTCTACCCTACCAACGCTGTGGGGAGAAAAAGTAGTACTGCGGATTTTAGATAGCTCTTCGGCGAATCTTG
ATATCAATATTTTGGGTTATAACGATAAGCAAAAATCTGATTATTTAACTGCCCTTGAACGCCCACAGGGAATGATATTA
ATTACTGGCCCAACAGGTAGCGGTAAAACCGTCTCTCTTTATACCGGGCTAAATATTTTAAATACCGATGAGCGAAATAT
TTCTACTGCTGAAGATCCGGTAGAAATTAACTTACCCGGTATTAACCAAGTACAAATTAATCAGCAAGTGGGTTTAAGTT
TTGCCGATGCGCTGCGTTCATTTCTGCGCCAAGATCCTGACATCGTGATGGTTGGTGAGATCCGTGATTTAGAAACAGCC
TCTATTGCGGTAAAAGCCGCCCAAACTGGTCACTTAGTATTATCAACCCTGCATACGAACTCTGCTGCTGAAACCATTAC
CCGTTTAACCAATATGGGGGTGGAAGATTTTAACCTCGCATCATCGCTGAGCTTAATCATCGCCCAGCGCCTTGCCCGTC
GCTTATGTAAAAGCTGTAAACACCCTCATCCACTGGATGAAATCACTTGCCAGCGTTTAAACGTGCCAATGAATACCACG
GTCTATCAAGCCAACAATGCAGGTTGTGATGATTGTAATAAAGGTTATGCCGGACGTGTCGGTATTTATGAAGTGATGGT
ATTTAGCCGCGAAATAGCCGAAGCCTTAATGGCGAATGCTACTGCATTACAACTCGAAGATATTGCTGTTAAACAAGGCA
TGCAGCGCTTAAAAGATTCAGGCATCGAGAAGCTACTTGAAGGCGTAACTAGCCTGTCTGAACTGCAACGTGTGTTACAG
CTTTAA

Domains

Predicted by InterproScan.

Type II secretion system (T2SS), protein E, N-terminal domain (MshEN)

(38-142)

Type II/IV secretion system protein (T2SSE)

(187-454)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  pilB Vibrio cholerae strain A1552

62.857

99.822

0.627
  pilB Vibrio parahaemolyticus RIMD 2210633

60

99.822

0.599
  pilB Vibrio campbellii strain DS40M4

59.643

99.822

0.595
  pilB Acinetobacter baumannii D1279779

53.191

92.157

0.49
  pilB Legionella pneumophila strain ERS1305867

48.227

100

0.485
  pilB Acinetobacter baylyi ADP1

51.724

93.048

0.481
  pilF Neisseria gonorrhoeae MS11

46.181

100

0.463
  pilF Thermus thermophilus HB27

37.876

100

0.381
  pilB/pilB1 Synechocystis sp. PCC 6803

40.504

91.979

0.373

Multiple sequence alignment