Detailed information    

insolico Bioinformatically predicted

Overview

Name   pilA   Type   Machinery gene
Locus tag   ACAK36_RS10430 Genome accession   NZ_AP031585
Coordinates   2129847..2130308 (-) Length   153 a.a.
NCBI ID   WP_000993718.1    Uniprot ID   A0A9P2XM63
Organism   Acinetobacter baumannii strain JUNP543     
Function   assembly of type IV pilus (predicted from homology)   
DNA binding and uptake

Genomic Context

Location: 2124847..2135308
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  ACAK36_RS10400 (JUNP543_2081) - 2125103..2125486 (+) 384 WP_000090000.1 RidA family protein -
  ACAK36_RS10405 (JUNP543_2082) - 2125696..2125890 (+) 195 WP_000289091.1 bacterioferritin-associated ferredoxin -
  ACAK36_RS10410 (JUNP543_2083) - 2126135..2126359 (+) 225 Protein_2043 ferritin-like domain-containing protein -
  ACAK36_RS10415 - 2126373..2127581 (-) 1209 WP_001206290.1 IS4-like element IS10A family transposase -
  ACAK36_RS10420 (JUNP543_2085) - 2127682..2127936 (+) 255 Protein_2045 ferritin-like domain-containing protein -
  ACAK36_RS10425 (JUNP543_2086) - 2127977..2129605 (-) 1629 WP_049589976.1 PglL family O-oligosaccharyltransferase -
  ACAK36_RS10430 (JUNP543_2087) pilA 2129847..2130308 (-) 462 WP_000993718.1 pilin Machinery gene
  ACAK36_RS10435 (JUNP543_2088) - 2130653..2131213 (-) 561 WP_049581048.1 TPM domain-containing protein -
  ACAK36_RS10440 (JUNP543_2089) - 2131207..2132289 (-) 1083 WP_049581051.1 YgcG family protein -
  ACAK36_RS10445 (JUNP543_2090) - 2132311..2132901 (-) 591 WP_000846931.1 LemA family protein -
  ACAK36_RS10450 (JUNP543_2091) - 2133032..2133919 (-) 888 WP_001017320.1 metal-dependent hydrolase -

Sequence

Protein


Download         Length: 153 a.a.        Molecular weight: 15449.52 Da        Isoelectric Point: 4.9218

>NTDB_id=110454 ACAK36_RS10430 WP_000993718.1 2129847..2130308(-) (pilA) [Acinetobacter baumannii strain JUNP543]
MNAQKGFTLIELMIVVAIIGILAAIAIPAYQNYVARSQATAGYSEISNMRTGYDTELNDGTAITSLSQVGFTATSSGACS
AIGVTNFGNDGAATNAITCTLKGNPKIAGKIISLSRSATGAWTCSTDIATTDEFLPKGCTGGGTPAVGAITTL

Nucleotide


Download         Length: 462 bp        

>NTDB_id=110454 ACAK36_RS10430 WP_000993718.1 2129847..2130308(-) (pilA) [Acinetobacter baumannii strain JUNP543]
ATGAATGCACAAAAAGGTTTTACATTAATCGAACTTATGATCGTGGTTGCCATTATTGGTATTTTGGCTGCGATTGCGAT
TCCTGCTTATCAAAATTATGTAGCTCGTTCTCAGGCTACAGCTGGATATTCTGAAATTAGTAACATGCGTACGGGTTACG
ACACCGAATTAAATGATGGTACAGCGATTACCTCTCTTTCTCAAGTTGGCTTTACTGCGACTTCATCTGGCGCATGTTCA
GCCATAGGTGTTACAAATTTTGGTAACGATGGCGCAGCTACTAACGCTATTACGTGTACTTTAAAAGGAAATCCAAAAAT
CGCTGGAAAGATTATTTCACTTTCACGTTCAGCGACCGGTGCATGGACTTGTAGTACAGATATTGCCACTACTGACGAAT
TCTTACCAAAAGGATGTACAGGTGGTGGAACACCAGCCGTTGGTGCAATTACTACTTTGTAA

Domains

Predicted by InterproScan.

Prokaryotic N-terminal methylation motif (N_methyl)

(1-26)

Pilin (bacterial filament) (Pilin)

(35-140)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  pilA Acinetobacter baumannii strain A118

64.286

100

0.647
  pilA Pseudomonas aeruginosa PAK

43.709

98.693

0.431
  pilA Vibrio cholerae strain A1552

42.581

100

0.431
  pilA Vibrio cholerae C6706

42.581

100

0.431
  pilA Vibrio cholerae O1 biovar El Tor strain E7946

42.581

100

0.431
  comP Acinetobacter baylyi ADP1

39.744

100

0.405
  pilA/pilAI Pseudomonas stutzeri DSM 10701

43.357

93.464

0.405

Multiple sequence alignment