Detailed information    

insolico Bioinformatically predicted

Overview

Name   pilC   Type   Machinery gene
Locus tag   ACNSN3_RS12735 Genome accession   NZ_CP183073
Coordinates   2727131..2728360 (+) Length   409 a.a.
NCBI ID   WP_418607612.1    Uniprot ID   -
Organism   Pseudoalteromonas sp. US3C1004     
Function   assembly of type IV pilus (predicted from homology)   
DNA binding and uptake

Genomic Context

Location: 2722131..2733360
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  ACNSN3_RS12715 (ACNSN3_12715) ampE 2723407..2724258 (-) 852 WP_055013474.1 beta-lactamase regulator AmpE -
  ACNSN3_RS12720 (ACNSN3_12720) ampD 2724280..2724825 (-) 546 WP_418607608.1 1,6-anhydro-N-acetylmuramyl-L-alanine amidase AmpD -
  ACNSN3_RS12725 (ACNSN3_12725) nadC 2725196..2726041 (+) 846 WP_418607610.1 carboxylating nicotinate-nucleotide diphosphorylase -
  ACNSN3_RS12730 (ACNSN3_12730) - 2726550..2726999 (+) 450 WP_010391865.1 pilin -
  ACNSN3_RS12735 (ACNSN3_12735) pilC 2727131..2728360 (+) 1230 WP_418607612.1 type II secretion system F family protein Machinery gene
  ACNSN3_RS12740 (ACNSN3_12740) pilD 2728610..2729521 (+) 912 WP_418607614.1 prepilin peptidase Machinery gene
  ACNSN3_RS12745 (ACNSN3_12745) coaE 2729524..2730153 (+) 630 WP_418607615.1 dephospho-CoA kinase -
  ACNSN3_RS12750 (ACNSN3_12750) pilB 2730288..2731964 (+) 1677 WP_418607617.1 type IV-A pilus assembly ATPase PilB Machinery gene
  ACNSN3_RS12755 (ACNSN3_12755) yacG 2732030..2732260 (+) 231 WP_010391853.1 DNA gyrase inhibitor YacG -
  ACNSN3_RS12760 (ACNSN3_12760) - 2732320..2732808 (-) 489 WP_418607619.1 Spy/CpxP family protein refolding chaperone -

Sequence

Protein


Download         Length: 409 a.a.        Molecular weight: 44255.24 Da        Isoelectric Point: 9.6165

>NTDB_id=1103963 ACNSN3_RS12735 WP_418607612.1 2727131..2728360(+) (pilC) [Pseudoalteromonas sp. US3C1004]
MANKQENKGLDTFQWVGVSARGKKLEGELTGSSIALVKAQLRKQGITPSKVKRKAKPLFGIKSVQKVTPKDIALVTRQIA
TMLMAGVPLIQSIEMIASGSTNKSVSKLMSAIGDEVKAGLPLSNALRKYPRYFDDLYCDLVASGEQSGALDKIFDRVALY
KEKSEALKSKIKKAMFYPISVLVVAGIVTSILLIFVVPQFQEIFAGFGAELPAFTLFVIAISEFMQEYWWMILIAVMAFG
YIYKEAALRSLALRDATDRAILKLPVIGMILNKAAVARYARTLSTTFAAGVPLVDALDSAAGASGNAVYRYAILEIKAEV
SSGNQMNWAMRNSKIFPDMVVQMVAIGEESGSLDGMLAKVATIYEQEVDDAVDGLSSLLEPLIMAVLGVLVGGLIVAMYL
PIFQLGSVI

Nucleotide


Download         Length: 1230 bp        

>NTDB_id=1103963 ACNSN3_RS12735 WP_418607612.1 2727131..2728360(+) (pilC) [Pseudoalteromonas sp. US3C1004]
ATGGCAAATAAGCAAGAAAATAAAGGCCTAGATACCTTTCAATGGGTAGGTGTAAGCGCTCGCGGCAAGAAGCTTGAGGG
TGAGCTTACTGGATCAAGCATTGCACTAGTCAAAGCGCAGTTACGCAAACAAGGCATTACTCCCTCAAAAGTTAAACGTA
AAGCCAAGCCGCTGTTTGGTATAAAAAGCGTACAAAAAGTAACCCCGAAAGATATTGCCTTAGTTACTCGACAAATTGCC
ACTATGCTAATGGCGGGTGTACCACTAATTCAGTCCATTGAGATGATAGCTTCAGGCTCCACCAATAAAAGCGTGAGTAA
GCTGATGAGTGCTATTGGCGATGAAGTCAAAGCAGGGTTGCCGCTTTCTAACGCACTTAGAAAATACCCTCGCTATTTTG
ATGATTTATATTGCGATTTAGTGGCCTCTGGTGAGCAATCTGGTGCACTGGATAAAATATTTGACCGCGTTGCGCTATAT
AAAGAAAAGTCAGAAGCACTAAAATCAAAAATTAAAAAAGCCATGTTTTATCCTATATCGGTTTTAGTGGTTGCAGGTAT
TGTAACTTCTATCTTGCTGATATTTGTAGTACCACAGTTTCAAGAAATATTTGCTGGTTTTGGCGCAGAGCTGCCCGCTT
TTACTTTATTTGTTATCGCCATTTCTGAATTTATGCAAGAGTATTGGTGGATGATATTGATTGCCGTAATGGCATTTGGC
TACATATATAAAGAAGCCGCACTCAGAAGTTTAGCGCTCAGAGATGCCACCGACCGCGCCATACTCAAGCTCCCTGTTAT
AGGTATGATTTTAAATAAAGCGGCCGTGGCACGTTATGCGCGTACTTTATCGACCACCTTTGCTGCGGGTGTACCGCTGG
TAGATGCGCTTGATTCTGCAGCCGGTGCCTCAGGTAATGCGGTTTATCGTTATGCTATTTTAGAAATAAAAGCTGAGGTA
AGCTCAGGTAATCAAATGAACTGGGCGATGCGTAATTCAAAAATATTCCCCGATATGGTGGTACAAATGGTCGCCATTGG
TGAAGAGTCAGGATCGCTTGATGGCATGCTGGCTAAAGTGGCCACTATTTATGAGCAAGAGGTGGATGACGCCGTAGATG
GCCTATCTAGCCTATTAGAGCCATTAATAATGGCGGTACTCGGCGTGCTAGTGGGTGGCTTAATTGTGGCCATGTACTTA
CCTATATTCCAACTAGGTTCAGTTATTTAG

Domains

Predicted by InterproScan.

Type II secretion system (T2SS), protein F (T2SSF)

(280-401)

Type II secretion system (T2SS), protein F (T2SSF)

(76-198)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  pilC Pseudomonas stutzeri DSM 10701

61.965

97.066

0.601
  pilC Acinetobacter baumannii D1279779

55.5

97.8

0.543
  pilC Acinetobacter baylyi ADP1

55

97.8

0.538
  pilC Legionella pneumophila strain ERS1305867

55.025

97.311

0.535
  pilC Vibrio cholerae strain A1552

45.545

98.778

0.45
  pilC Vibrio campbellii strain DS40M4

43.5

97.8

0.425
  pilG Neisseria meningitidis 44/76-A

44.246

95.599

0.423
  pilG Neisseria gonorrhoeae MS11

43.99

95.599

0.421

Multiple sequence alignment