Detailed information    

insolico Bioinformatically predicted

Overview

Name   pilB   Type   Machinery gene
Locus tag   ACNQFN_RS17820 Genome accession   NZ_CP183038
Coordinates   3680085..3681803 (+) Length   572 a.a.
NCBI ID   WP_068637468.1    Uniprot ID   -
Organism   Thauera butanivorans strain TB     
Function   power the assembly of type IV pilus (predicted from homology)   
DNA binding and uptake

Genomic Context

Location: 3675085..3686803
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  ACNQFN_RS17795 (ACNQFN_17795) - 3675373..3676176 (-) 804 WP_418647141.1 M48 family metallopeptidase -
  ACNQFN_RS17800 (ACNQFN_17800) - 3676173..3676922 (-) 750 WP_068637475.1 competence/damage-inducible protein A -
  ACNQFN_RS17805 (ACNQFN_17805) - 3676919..3677695 (-) 777 WP_418647142.1 EI24 domain-containing protein -
  ACNQFN_RS17810 (ACNQFN_17810) ffh 3677718..3679088 (-) 1371 WP_068637472.1 signal recognition particle protein -
  ACNQFN_RS17815 (ACNQFN_17815) - 3679140..3679973 (+) 834 WP_068637470.1 cytochrome C assembly family protein -
  ACNQFN_RS17820 (ACNQFN_17820) pilB 3680085..3681803 (+) 1719 WP_068637468.1 type IV-A pilus assembly ATPase PilB Machinery gene
  ACNQFN_RS17825 (ACNQFN_17825) pilC 3681824..3683053 (+) 1230 WP_068637466.1 type II secretion system F family protein Machinery gene
  ACNQFN_RS17830 (ACNQFN_17830) pilD 3683061..3683912 (+) 852 WP_068637464.1 prepilin peptidase Machinery gene
  ACNQFN_RS17835 (ACNQFN_17835) - 3684068..3684337 (+) 270 WP_068637462.1 FmdB family zinc ribbon protein -
  ACNQFN_RS17840 (ACNQFN_17840) - 3684398..3685021 (+) 624 WP_068637461.1 DUF502 domain-containing protein -

Sequence

Protein


Download         Length: 572 a.a.        Molecular weight: 62267.37 Da        Isoelectric Point: 5.0148

>NTDB_id=1103741 ACNQFN_RS17820 WP_068637468.1 3680085..3681803(+) (pilB) [Thauera butanivorans strain TB]
MAANPQSALSGFARALVQHGRISEADAIACTPRDAEGANAFMLEIAQRGLLSAAAMARFAADTFGYPLVDIAAFDKATFS
IDAVDRKLMQKHQVVALAKRQNRITLALADPSSMRALDEIRFQTGMQLDLVVAEADKLKRVVDGLSESASETLKELTGDD
FDMDLLNQEAPGEAQAEEESSNDVDDAPVVRFIQKVLIDAINEGASDVHFEPYEKYYRIRVRTDGTLREIAQPPLVLKEK
IAARIKVISRLDISEKRVPQDGRMKLVLSKNKAIDFRVSTLPTLHGEKIVMRILDPSSAMLGIDALGYEPDQRKALLDAI
ERPYGMILVTGPTGSGKTVSLYTCLNLLNKAGVNISTAEDPAEINLPGINQVNVNEKAGLTFAASLRAFLRQDPDVIMVG
EIRDLETAEISIKAAQTGHLVLSTLHTNDAPTTLERLKNMGVAPFNIASSVILITAQRLARRLCSCKKPADIPIEAMLEA
GFTVEDLDGSWQPYAPVGCERCKDSGYKGRVGIYQVMPISEEIAHIIMTGGNSMDIAAQAEREGVRDLRKSGLLKVRQGV
TSLEEVLATTNE

Nucleotide


Download         Length: 1719 bp        

>NTDB_id=1103741 ACNQFN_RS17820 WP_068637468.1 3680085..3681803(+) (pilB) [Thauera butanivorans strain TB]
ATGGCGGCAAATCCTCAATCAGCCCTGAGCGGCTTCGCTCGGGCTCTCGTTCAGCACGGACGCATTTCCGAGGCCGACGC
GATCGCCTGCACGCCACGGGACGCCGAAGGCGCCAACGCCTTCATGCTCGAAATCGCGCAGCGCGGCCTGCTGTCCGCCG
CGGCCATGGCCCGCTTCGCCGCCGACACCTTCGGCTATCCGCTGGTCGACATCGCCGCCTTCGACAAAGCGACGTTCTCG
ATCGACGCGGTCGACCGCAAGCTGATGCAGAAGCATCAGGTCGTGGCCCTGGCCAAGCGCCAGAACCGCATCACGCTCGC
CCTCGCCGATCCGTCCAGCATGCGCGCGCTGGACGAGATCCGCTTCCAGACCGGCATGCAGCTCGATCTGGTGGTTGCCG
AAGCCGACAAGCTCAAGCGCGTGGTGGACGGCTTGTCGGAATCCGCCTCGGAAACGCTGAAGGAACTCACCGGCGACGAT
TTCGACATGGACCTCCTGAACCAGGAGGCGCCGGGCGAGGCACAGGCGGAAGAGGAAAGCAGCAACGACGTCGACGACGC
GCCGGTCGTCCGTTTCATCCAGAAAGTGCTGATCGACGCGATCAACGAAGGCGCGTCCGACGTTCACTTCGAGCCTTACG
AGAAGTACTACCGCATCCGCGTACGCACCGACGGCACGCTGCGCGAGATCGCCCAGCCGCCGCTGGTGCTGAAGGAAAAG
ATCGCCGCCCGCATCAAGGTCATCTCGCGCCTGGACATCTCCGAGAAGCGCGTTCCCCAGGACGGACGCATGAAACTGGT
GCTGTCCAAGAACAAGGCCATCGACTTCCGCGTGTCGACGCTGCCGACCCTGCACGGCGAGAAGATCGTGATGCGTATCC
TCGACCCGAGTTCGGCGATGCTGGGCATCGATGCGCTCGGCTACGAGCCGGACCAGCGCAAGGCCCTGCTCGACGCCATC
GAACGGCCCTACGGCATGATCCTCGTCACCGGCCCGACCGGCTCCGGCAAGACCGTATCGCTGTACACCTGCCTCAACCT
GCTGAACAAGGCAGGCGTCAATATCTCGACCGCGGAAGACCCGGCGGAAATCAACCTGCCCGGCATCAACCAGGTCAACG
TCAACGAGAAGGCCGGGCTGACTTTCGCCGCGTCCCTGCGCGCCTTCCTGCGCCAGGATCCGGACGTGATCATGGTCGGC
GAGATCCGCGACCTCGAAACCGCCGAGATCTCGATCAAGGCAGCGCAGACCGGCCACCTCGTGCTGTCCACGCTGCACAC
CAACGACGCACCGACCACGCTCGAACGCCTCAAGAACATGGGCGTGGCGCCGTTCAATATTGCCTCTTCAGTGATCCTGA
TCACGGCGCAACGCCTGGCGCGGCGGTTATGCTCCTGCAAGAAACCGGCCGACATACCGATCGAAGCGATGCTGGAAGCC
GGTTTCACGGTGGAAGACCTCGACGGCAGCTGGCAGCCCTATGCGCCAGTGGGTTGCGAGCGCTGCAAGGACAGCGGTTA
CAAGGGACGGGTTGGCATCTATCAGGTCATGCCCATCAGCGAGGAAATTGCTCACATCATCATGACGGGTGGGAACTCCA
TGGACATCGCCGCACAGGCCGAACGCGAAGGCGTGCGCGACCTGCGCAAGTCCGGCCTGCTCAAGGTCAGGCAGGGCGTC
ACCTCACTCGAAGAAGTGCTGGCCACGACCAACGAATAA

Domains

Predicted by InterproScan.

Type II/IV secretion system protein (T2SSE)

(195-462)

Type II secretion system (T2SS), protein E, N-terminal domain (MshEN)

(46-149)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  pilB Acinetobacter baumannii D1279779

55.712

99.476

0.554
  pilB Acinetobacter baylyi ADP1

54.833

99.476

0.545
  pilB Legionella pneumophila strain ERS1305867

52.734

99.126

0.523
  pilF Neisseria gonorrhoeae MS11

51.579

99.65

0.514
  pilB Vibrio cholerae strain A1552

52.268

88.636

0.463
  pilB Vibrio parahaemolyticus RIMD 2210633

48.566

91.434

0.444
  pilB Vibrio campbellii strain DS40M4

48.19

91.783

0.442
  pilF Thermus thermophilus HB27

38.407

98.776

0.379
  pilB Deinococcus radiodurans R1 = ATCC 13939 = DSM 20539

39.662

93.007

0.369

Multiple sequence alignment