Detailed information    

insolico Bioinformatically predicted

Overview

Name   pilD   Type   Machinery gene
Locus tag   ACNQFN_RS17830 Genome accession   NZ_CP183038
Coordinates   3683061..3683912 (+) Length   283 a.a.
NCBI ID   WP_068637464.1    Uniprot ID   -
Organism   Thauera butanivorans strain TB     
Function   assembly of type IV pilus (predicted from homology)   
DNA binding and uptake

Genomic Context

Location: 3678061..3688912
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  ACNQFN_RS17815 (ACNQFN_17815) - 3679140..3679973 (+) 834 WP_068637470.1 cytochrome C assembly family protein -
  ACNQFN_RS17820 (ACNQFN_17820) pilB 3680085..3681803 (+) 1719 WP_068637468.1 type IV-A pilus assembly ATPase PilB Machinery gene
  ACNQFN_RS17825 (ACNQFN_17825) pilC 3681824..3683053 (+) 1230 WP_068637466.1 type II secretion system F family protein Machinery gene
  ACNQFN_RS17830 (ACNQFN_17830) pilD 3683061..3683912 (+) 852 WP_068637464.1 prepilin peptidase Machinery gene
  ACNQFN_RS17835 (ACNQFN_17835) - 3684068..3684337 (+) 270 WP_068637462.1 FmdB family zinc ribbon protein -
  ACNQFN_RS17840 (ACNQFN_17840) - 3684398..3685021 (+) 624 WP_068637461.1 DUF502 domain-containing protein -
  ACNQFN_RS17845 (ACNQFN_17845) aspS 3685084..3686883 (+) 1800 WP_068637459.1 aspartate--tRNA ligase -
  ACNQFN_RS17850 (ACNQFN_17850) - 3687067..3688716 (+) 1650 WP_068637457.1 response regulator -

Sequence

Protein


Download         Length: 283 a.a.        Molecular weight: 30663.19 Da        Isoelectric Point: 7.7658

>NTDB_id=1103743 ACNQFN_RS17830 WP_068637464.1 3683061..3683912(+) (pilD) [Thauera butanivorans strain TB]
MSEALRDPIAFTVLGALLGLFVGSFLNVVIHRLPRMMEREWHAQAAELRGEPAPAAERFNLATPRSRCPHCGHLIGAFEN
IPVVSYLVLRGRCRHCGAGISKRYPIVEAFTAALSGYAAWHFGFGLTALGALLFIWTMVALAFIDLDTQLLPDELTLPLL
WLGLLFNLGETFTDLPSAVAGAMAGYLALWSVYWTFKLLTGKEGMGYGDFKLLAAIGAWLGWQALPLTILFSSLVGALAG
VALIVVARRGRDVPIPFGPYLAAAGVIALFWGEALSTRYLGML

Nucleotide


Download         Length: 852 bp        

>NTDB_id=1103743 ACNQFN_RS17830 WP_068637464.1 3683061..3683912(+) (pilD) [Thauera butanivorans strain TB]
ATGTCCGAGGCTCTTCGCGACCCCATCGCCTTCACCGTACTGGGCGCCCTGCTCGGGCTGTTCGTCGGCAGCTTCCTCAA
TGTGGTGATCCACCGCCTGCCCAGGATGATGGAGCGCGAATGGCATGCCCAGGCGGCGGAGCTTCGCGGCGAACCTGCGC
CGGCGGCCGAACGCTTCAACCTCGCCACGCCGCGCTCGCGCTGCCCGCACTGCGGCCACCTGATCGGCGCCTTCGAGAAC
ATTCCCGTCGTCAGCTACCTGGTCCTGCGCGGCCGCTGCCGCCACTGCGGTGCCGGCATCAGCAAGCGCTACCCGATCGT
CGAGGCCTTCACCGCCGCACTGTCGGGCTACGCAGCCTGGCACTTCGGCTTCGGCCTCACCGCGCTCGGCGCCCTGCTCT
TCATCTGGACGATGGTGGCGCTTGCCTTCATCGACCTCGACACCCAGCTCCTTCCCGATGAACTGACGCTGCCGCTGCTG
TGGCTCGGCCTGCTGTTCAATCTGGGCGAGACATTCACCGATCTGCCGAGCGCGGTCGCCGGCGCGATGGCCGGTTATCT
CGCGCTGTGGTCGGTCTATTGGACCTTCAAGCTGCTGACCGGCAAGGAAGGCATGGGCTACGGCGACTTCAAGCTGCTGG
CGGCGATCGGCGCCTGGCTGGGCTGGCAGGCGCTGCCATTGACGATCCTGTTCTCGTCGCTGGTCGGCGCGCTGGCCGGT
GTCGCGCTGATCGTCGTCGCCCGCCGCGGCCGCGATGTCCCGATCCCCTTCGGCCCCTATCTCGCCGCGGCGGGCGTGAT
CGCGCTGTTCTGGGGCGAAGCCCTGAGCACCCGCTACCTCGGCATGCTGTAG

Domains

Predicted by InterproScan.

Bacterial Peptidase A24 N-terminal domain (A24_N_bact)

(17-122)

Type IV leader peptidase family (Peptidase_A24)

(133-240)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  pilD Vibrio cholerae strain A1552

55.636

97.173

0.541
  pilD Vibrio campbellii strain DS40M4

52.536

97.527

0.512
  pilD Neisseria gonorrhoeae MS11

52.344

90.459

0.474
  pilD Acinetobacter baumannii D1279779

47.67

98.587

0.47
  pilD Acinetobacter nosocomialis M2

47.312

98.587

0.466

Multiple sequence alignment