Detailed information    

insolico Bioinformatically predicted

Overview

Name   pilQ   Type   Machinery gene
Locus tag   ACM1ZW_RS10900 Genome accession   NZ_CP182230
Coordinates   2349040..2351139 (+) Length   699 a.a.
NCBI ID   WP_416740524.1    Uniprot ID   -
Organism   Pseudomonas sp. NFX71     
Function   assembly of type IV pilus (predicted from homology)   
DNA binding and uptake

Genomic Context

Location: 2344040..2356139
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  ACM1ZW_RS10880 (ACM1ZW_10880) comM 2346252..2347316 (+) 1065 WP_017336120.1 pilus assembly protein PilM Machinery gene
  ACM1ZW_RS10885 (ACM1ZW_10885) - 2347316..2347882 (+) 567 WP_416740522.1 PilN domain-containing protein -
  ACM1ZW_RS10890 (ACM1ZW_10890) pilO 2347879..2348502 (+) 624 WP_017336122.1 type 4a pilus biogenesis protein PilO -
  ACM1ZW_RS10895 (ACM1ZW_10895) - 2348499..2349026 (+) 528 WP_416740523.1 pilus assembly protein PilP -
  ACM1ZW_RS10900 (ACM1ZW_10900) pilQ 2349040..2351139 (+) 2100 WP_416740524.1 type IV pilus secretin PilQ Machinery gene
  ACM1ZW_RS10905 (ACM1ZW_10905) aroK 2351144..2351662 (+) 519 WP_017336125.1 shikimate kinase AroK -
  ACM1ZW_RS10910 (ACM1ZW_10910) aroB 2351814..2352914 (+) 1101 WP_017336126.1 3-dehydroquinate synthase -
  ACM1ZW_RS10915 (ACM1ZW_10915) - 2352925..2354523 (+) 1599 WP_017336127.1 AAA family ATPase -

Sequence

Protein


Download         Length: 699 a.a.        Molecular weight: 75489.73 Da        Isoelectric Point: 5.5570

>NTDB_id=1100917 ACM1ZW_RS10900 WP_416740524.1 2349040..2351139(+) (pilQ) [Pseudomonas sp. NFX71]
MNRIFATFGVSLWIALLSPMVQAANLKALDVAALPGDRVELKLSFDGAPPAPHGYTTEQPARIALDLPGVVSQLTSKTRD
LGGGNARSATVVEAKDRTRLIINLTQLTPYTARVEGNNLFVVVGRGTSNAASAPAPATAPKPPRVATATPAPAKAYAPVS
KAIRGVDFQRGTQGEGNVVIDLSDPSIAPDIQERDGRIVVGFARTQLPEPLRVRLDVKDFATPVQFVNASATGDRATISI
EPSGAFDYSTYQTDNKLTVSVRPMTVDDLQKRNAERFAYTGEKLSLNFQDIDVRSVLQLIADFTNLNLVASDTVQGGITL
RLQNVPWDQALDLVLKTKGLDKRKIGNVLLVAPADEIAARERQELESQKQIAELAPLRRELLQVNYAKAADIAKLFQSVT
SAEAKVDERGSITVDERTNNIIAYQTQDRLDELRRIVAQLDIPVRQVMIEARIVEANVDYDKSLGVRWGGSVQNKGNWNT
SGVSNGANASSTIGTPGSTSTNSPFVDMGTAANTSGLGIAFITDNVLLDLELTAMEKTGNGEIVSQPKVVTSDKETAKIL
KGTEIPYQEASSSGATSVSFKEASLSLEVTPQITPDNRIIMEVKVTKDEPDYLNKVQDVPPIKKNEVNAKVLVNDGETIV
IGGVFSNTQSKVVDKVPFLGDVPYLGRLFRRDVVSEKKSELLVFLTPRIMNNQAIAVSH

Nucleotide


Download         Length: 2100 bp        

>NTDB_id=1100917 ACM1ZW_RS10900 WP_416740524.1 2349040..2351139(+) (pilQ) [Pseudomonas sp. NFX71]
ATGAACAGGATTTTCGCAACCTTCGGAGTTTCGCTATGGATAGCGTTGCTTTCGCCGATGGTACAAGCGGCCAACCTCAA
AGCGCTGGATGTCGCTGCGCTGCCGGGAGACCGCGTCGAGTTGAAGCTGTCATTCGATGGCGCGCCTCCAGCGCCCCACG
GTTACACGACGGAGCAGCCTGCGCGGATCGCGCTGGACCTGCCTGGTGTGGTCAGCCAGCTAACGAGCAAGACTCGCGAC
CTGGGCGGCGGCAATGCCCGCAGTGCCACGGTGGTGGAAGCCAAGGACCGCACGCGGCTGATCATCAACCTGACTCAATT
GACCCCGTACACCGCTCGTGTCGAAGGCAATAACCTGTTTGTGGTGGTCGGGCGCGGAACCAGTAACGCCGCCTCTGCAC
CTGCACCTGCGACCGCACCCAAGCCGCCACGCGTCGCCACGGCAACTCCCGCGCCGGCCAAGGCCTATGCGCCGGTGAGC
AAGGCGATCCGCGGCGTGGATTTCCAGCGCGGCACCCAGGGTGAAGGCAATGTGGTCATCGATCTCTCGGATCCGTCCAT
CGCCCCGGACATTCAGGAGCGCGATGGCAGGATTGTCGTCGGTTTCGCCAGGACCCAATTGCCGGAACCGTTGCGGGTCC
GTCTGGACGTCAAGGATTTCGCCACACCCGTGCAATTCGTCAACGCCAGCGCCACGGGAGACCGGGCGACCATCAGTATT
GAGCCCAGCGGCGCGTTCGATTACTCGACCTATCAAACCGACAACAAGCTGACCGTCAGCGTCCGCCCGATGACCGTAGA
CGACTTGCAGAAGCGCAACGCCGAGCGTTTTGCCTACACCGGTGAAAAGCTCTCGCTGAACTTCCAGGACATTGACGTGC
GCTCGGTGCTGCAACTGATCGCCGATTTCACCAACCTCAATCTGGTGGCCAGCGACACGGTGCAGGGCGGCATTACCTTG
CGCTTGCAGAACGTGCCGTGGGATCAGGCGCTGGACCTGGTGCTGAAAACCAAGGGCCTGGACAAACGAAAGATCGGTAA
CGTGTTGCTGGTGGCGCCGGCCGATGAGATTGCCGCCCGCGAACGCCAGGAACTGGAGTCGCAGAAGCAGATCGCCGAAC
TGGCGCCTTTGCGTCGCGAGCTGCTGCAAGTCAATTACGCCAAGGCGGCCGATATCGCCAAGCTGTTCCAGTCAGTGACC
AGTGCCGAAGCGAAAGTCGACGAGCGGGGTTCGATCACCGTCGATGAACGAACCAATAACATCATCGCCTACCAGACCCA
GGACCGTCTCGACGAGCTGCGCCGTATTGTCGCGCAACTGGATATCCCGGTGCGTCAGGTGATGATCGAGGCTCGGATCG
TCGAAGCGAACGTCGACTACGACAAGAGCCTCGGTGTGCGTTGGGGTGGTTCGGTGCAGAACAAGGGCAACTGGAACACA
TCCGGTGTCAGCAACGGCGCCAACGCGTCGTCGACCATTGGTACGCCGGGCAGCACCAGCACCAACTCACCGTTCGTCGA
CATGGGTACCGCCGCCAACACTTCGGGGCTCGGCATCGCCTTCATCACCGATAATGTGCTGCTGGATCTTGAGCTGACCG
CGATGGAAAAAACCGGTAACGGCGAAATCGTCTCGCAACCCAAGGTGGTTACCTCCGACAAGGAAACCGCAAAAATCCTC
AAGGGCACCGAGATCCCGTACCAGGAAGCCAGCTCCAGCGGCGCTACGTCGGTATCGTTCAAGGAGGCCTCGCTGTCGCT
GGAAGTGACGCCGCAGATTACCCCGGACAACCGGATCATCATGGAGGTCAAGGTCACCAAGGACGAACCGGACTACCTGA
ACAAGGTGCAGGATGTACCGCCCATCAAGAAAAACGAGGTCAATGCCAAGGTTCTCGTCAATGACGGCGAGACCATCGTG
ATCGGTGGGGTTTTCTCAAATACTCAGAGCAAGGTTGTAGATAAGGTGCCATTTCTCGGTGATGTGCCGTATCTTGGCCG
CCTTTTCCGGCGTGACGTGGTTTCGGAGAAAAAATCCGAGCTGCTGGTATTTCTCACTCCGCGTATCATGAACAATCAGG
CGATTGCTGTGAGTCATTGA

Domains

Predicted by InterproScan.

AMIN domain (AMIN)

(37-122)

Bacterial type II and III secretion system protein (Secretin)

(534-690)

Secretin and TonB N terminus short domain (STN)

(307-353)

Bacterial type II/III secretion system short domain (Secretin_N)

(381-446)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  pilQ Pseudomonas aeruginosa PAK

72.702

100

0.747
  comQ Acinetobacter baylyi ADP1

41.619

100

0.419
  pilQ Acinetobacter baumannii strain A118

38.127

100

0.413
  pilQ Acinetobacter baumannii D1279779

38.127

100

0.413
  pilQ Legionella pneumophila strain Lp02

38.416

97.568

0.375
  pilQ Legionella pneumophila strain ERS1305867

38.416

97.568

0.375

Multiple sequence alignment