Detailed information    

insolico Bioinformatically predicted

Overview

Name   comM   Type   Machinery gene
Locus tag   ACM1ZW_RS10880 Genome accession   NZ_CP182230
Coordinates   2346252..2347316 (+) Length   354 a.a.
NCBI ID   WP_017336120.1    Uniprot ID   A0A0K1QKG0
Organism   Pseudomonas sp. NFX71     
Function   assembly of type IV pilus (predicted from homology)   
DNA binding and uptake

Genomic Context

Location: 2341252..2352316
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  ACM1ZW_RS10870 (ACM1ZW_10870) - 2342157..2343425 (+) 1269 WP_017336118.1 malic enzyme-like NAD(P)-binding protein -
  ACM1ZW_RS10875 (ACM1ZW_10875) - 2343596..2346034 (-) 2439 WP_416740521.1 penicillin-binding protein 1A -
  ACM1ZW_RS10880 (ACM1ZW_10880) comM 2346252..2347316 (+) 1065 WP_017336120.1 pilus assembly protein PilM Machinery gene
  ACM1ZW_RS10885 (ACM1ZW_10885) - 2347316..2347882 (+) 567 WP_416740522.1 PilN domain-containing protein -
  ACM1ZW_RS10890 (ACM1ZW_10890) pilO 2347879..2348502 (+) 624 WP_017336122.1 type 4a pilus biogenesis protein PilO -
  ACM1ZW_RS10895 (ACM1ZW_10895) - 2348499..2349026 (+) 528 WP_416740523.1 pilus assembly protein PilP -
  ACM1ZW_RS10900 (ACM1ZW_10900) pilQ 2349040..2351139 (+) 2100 WP_416740524.1 type IV pilus secretin PilQ Machinery gene
  ACM1ZW_RS10905 (ACM1ZW_10905) aroK 2351144..2351662 (+) 519 WP_017336125.1 shikimate kinase AroK -

Sequence

Protein


Download         Length: 354 a.a.        Molecular weight: 38083.53 Da        Isoelectric Point: 4.7245

>NTDB_id=1100916 ACM1ZW_RS10880 WP_017336120.1 2346252..2347316(+) (comM) [Pseudomonas sp. NFX71]
MLGLFNKKANTLLGIDISSTSVKLLELSRQGDRYRVEAYAVEPLPASAVVEKNIAELEGVGQALTRVLLKARTSLRNVAV
AVAGSAVITKTIEMDAGLSDDELENQLKIEADQYIPYPLDEVAIDFEVQGASARNPERVNVLLAACRKENVEVREAALAL
AGLTARVVDVEAYALERSFGLLAGQLAAAQDRLTVAVVDIGATMTTLSVLYNGRIIYTREQLFGGRQLTEEIQRRYGLTL
EQAGLAKKQGGLPDDYVSEVLQPFRDALVQQVSRSLQFFFASGQYNAVDHILLAGGTASVPGLDRLIEQRLSTPTQVANP
FSNMTLSSKVNAGALASDAPALMIACGLALRSFD

Nucleotide


Download         Length: 1065 bp        

>NTDB_id=1100916 ACM1ZW_RS10880 WP_017336120.1 2346252..2347316(+) (comM) [Pseudomonas sp. NFX71]
GTGCTAGGACTCTTCAATAAAAAGGCCAATACGCTTTTGGGGATCGACATCAGCTCTACGTCGGTGAAGCTTCTGGAGTT
AAGCCGCCAGGGCGACCGCTACCGGGTCGAAGCCTATGCGGTAGAGCCGTTGCCTGCCAGCGCGGTGGTCGAGAAAAATA
TCGCCGAGCTCGAAGGCGTGGGTCAGGCACTCACGCGCGTGCTGCTCAAGGCCAGAACCAGTCTCAGGAACGTCGCAGTG
GCCGTTGCCGGCTCGGCGGTGATCACCAAGACCATCGAAATGGATGCCGGTCTTTCCGACGACGAGTTGGAAAACCAGCT
GAAAATCGAGGCTGATCAATACATCCCTTATCCGCTGGACGAAGTGGCCATCGACTTCGAAGTCCAGGGTGCATCGGCGC
GCAACCCCGAACGGGTCAATGTGTTGCTGGCGGCCTGTCGCAAGGAAAACGTCGAAGTCCGTGAGGCGGCGCTCGCACTG
GCCGGCCTGACCGCTCGCGTGGTCGATGTCGAGGCCTATGCGCTGGAGCGATCCTTCGGTTTGCTGGCCGGACAGCTGGC
AGCCGCTCAGGACCGTCTGACGGTTGCCGTGGTCGACATCGGTGCGACCATGACCACGCTCAGCGTCCTGTACAACGGAC
GCATCATCTACACCCGCGAACAATTGTTCGGTGGTCGTCAACTGACGGAAGAAATCCAGCGACGTTATGGCCTGACCCTC
GAGCAAGCAGGGTTGGCAAAGAAGCAGGGCGGTTTGCCGGACGACTACGTCAGCGAGGTGCTGCAGCCATTTCGTGATGC
GCTGGTGCAGCAGGTGTCGCGATCGTTGCAGTTTTTCTTCGCCTCCGGGCAGTACAACGCTGTGGATCACATCCTGTTGG
CCGGTGGTACCGCTTCGGTGCCGGGGCTGGACCGGTTGATCGAGCAGCGTTTGAGCACGCCGACGCAGGTGGCCAATCCC
TTCTCGAACATGACCCTGAGCAGCAAGGTCAACGCTGGCGCCCTGGCCAGCGATGCGCCGGCGCTGATGATCGCTTGCGG
GCTGGCCCTCAGGAGTTTCGACTGA

Domains

Predicted by InterproScan.

Type IV pilus assembly protein PilM; (PilM_2)

(14-353)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure
  AlphaFold DB A0A0K1QKG0

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  comM Acinetobacter nosocomialis M2

57.062

100

0.571
  pilM Acinetobacter baumannii D1279779

56.78

100

0.568
  comM Acinetobacter baylyi ADP1

54.52

100

0.545
  pilM Legionella pneumophila strain ERS1305867

48.732

100

0.489

Multiple sequence alignment