Detailed information    

insolico Bioinformatically predicted

Overview

Name   pilB   Type   Machinery gene
Locus tag   ACBO77_RS16485 Genome accession   NZ_CP182134
Coordinates   3814839..3816572 (-) Length   577 a.a.
NCBI ID   WP_039512338.1    Uniprot ID   A0AAQ1AK58
Organism   Xanthomonas arboricola pv. pruni strain Xap 9-4     
Function   power the assembly of type IV pilus (predicted from homology)   
DNA binding and uptake

Related MGE

Note: This gene co-localizes with putative mobile genetic elements (MGEs) in the genome predicted by VRprofile2, as detailed below.

Gene-MGE association summary

MGE type MGE coordinates Gene coordinates Relative position Distance (bp)
ICE 3814839..3838003 3814839..3816572 within 0


Gene organization within MGE regions


Location: 3814839..3838003
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  ACBO77_RS16485 pilB 3814839..3816572 (-) 1734 WP_039512338.1 type IV-A pilus assembly ATPase PilB Machinery gene
  ACBO77_RS16490 - 3816757..3817161 (-) 405 WP_043088084.1 pilin -
  ACBO77_RS16495 pilC 3817539..3818798 (+) 1260 WP_043088082.1 type II secretion system F family protein Machinery gene
  ACBO77_RS16500 - 3818805..3819668 (+) 864 WP_016849610.1 prepilin peptidase -
  ACBO77_RS16505 coaE 3819682..3820305 (+) 624 WP_043088081.1 dephospho-CoA kinase -
  ACBO77_RS16510 - 3820315..3820560 (+) 246 WP_372160084.1 SymE family type I addiction module toxin -
  ACBO77_RS16515 - 3820666..3822000 (-) 1335 WP_016904089.1 sensor histidine kinase -
  ACBO77_RS16520 - 3821993..3822670 (-) 678 WP_006448355.1 response regulator transcription factor -
  ACBO77_RS16525 - 3822771..3823130 (-) 360 WP_223571086.1 hypothetical protein -
  ACBO77_RS16530 rimK 3823357..3824262 (-) 906 WP_166767257.1 30S ribosomal protein S6--L-glutamate ligase -
  ACBO77_RS16535 glgX 3824753..3826885 (+) 2133 WP_039516331.1 glycogen debranching protein GlgX -
  ACBO77_RS16540 - 3827557..3827949 (-) 393 WP_023904915.1 H-NS family nucleoid-associated regulatory protein -
  ACBO77_RS16545 - 3828038..3828424 (-) 387 WP_026064907.1 hypothetical protein -
  ACBO77_RS16550 - 3829240..3829581 (+) 342 WP_043088080.1 hypothetical protein -
  ACBO77_RS16555 - 3829634..3831235 (-) 1602 WP_039516320.1 MobA/MobL family protein -
  ACBO77_RS16560 - 3831501..3831755 (+) 255 WP_039516317.1 hypothetical protein -
  ACBO77_RS16565 - 3831783..3833216 (-) 1434 WP_043088079.1 hypothetical protein -
  ACBO77_RS16570 - 3833209..3833886 (-) 678 WP_416639822.1 hypothetical protein -
  ACBO77_RS16575 - 3834923..3835552 (+) 630 WP_039516323.1 hypothetical protein -
  ACBO77_RS16580 - 3835552..3836148 (+) 597 WP_080640147.1 hypothetical protein -
  ACBO77_RS16585 - 3836896..3837243 (+) 348 Protein_3242 integrase -

Sequence

Protein


Download         Length: 577 a.a.        Molecular weight: 62578.76 Da        Isoelectric Point: 5.6006

>NTDB_id=1100525 ACBO77_RS16485 WP_039512338.1 3814839..3816572(-) (pilB) [Xanthomonas arboricola pv. pruni strain Xap 9-4]
MNVVIANLVGITGIARRLVQDGALDESAARLAMDQASAAKVPLPQWFAEKKLVTASQLAAANAVEFGMPLMDVSVFDANQ
NAVKLVSEELLQKYQVLPLFKRGNRLFVGVSNPTQTRALDDIKFHTNLVVEPILVDEDQIRRTLEQWQASNASLGSSLGD
DDEGMGDLDVSAGDEDMGAGGDSGVDAKGDDTPVVKFVNKVLVDAIRRGASDIHFEPYEDDYRVRLRIDGLLKNVAKAPV
KLSQRIAARLKVMSQLDIAEKRVPQDGRIKLNLSKTKQIDFRVSTLPTLFGEKVVLRILDGSAAKLGIEKLGYEADQQKL
FLEAIHKPYGMVLVTGPTGSGKTVSLYTALGILNDETRNISTAEDPVEIRLPGVNQVQQNNKRGMTFAAALRSFLRQDPD
IIMVGEIRDLETAEIAIKAAQTGHMVLSTLHTNDAPQTIARLMNMGIAPYNITSSVTLVIAQRLARRLCNNCKRKSTLPE
HALLAEGFTPAQIAAGIELYEAVGCDECTEGYKGRTGIYQVMPMTDEIGAIVLEGGNAMQIAEAAQSIGIRDLRQSALVK
AAHGVTSLAEINRVTKD

Nucleotide


Download         Length: 1734 bp        

>NTDB_id=1100525 ACBO77_RS16485 WP_039512338.1 3814839..3816572(-) (pilB) [Xanthomonas arboricola pv. pruni strain Xap 9-4]
ATGAACGTAGTCATTGCCAATCTGGTCGGCATTACCGGCATTGCTCGTCGGCTTGTCCAAGACGGGGCTTTAGATGAGTC
AGCGGCAAGGCTGGCGATGGATCAGGCTAGCGCTGCCAAGGTGCCTCTGCCCCAATGGTTTGCTGAGAAAAAATTGGTAA
CCGCGTCTCAACTTGCCGCCGCTAACGCGGTCGAATTCGGTATGCCACTGATGGACGTGTCGGTCTTCGACGCCAATCAG
AACGCGGTCAAACTGGTCAGTGAGGAGTTGCTCCAGAAGTATCAGGTACTGCCGCTGTTCAAGCGCGGCAACCGGTTGTT
CGTGGGGGTGAGCAACCCGACCCAGACCAGGGCGCTGGACGACATCAAGTTTCATACCAATTTGGTGGTCGAGCCGATCC
TGGTTGACGAGGACCAGATCCGTCGTACCTTGGAGCAGTGGCAGGCCAGCAACGCATCGTTGGGCTCGTCGCTCGGGGAC
GATGATGAGGGGATGGGCGACCTGGACGTGTCGGCCGGGGACGAGGACATGGGCGCCGGCGGGGATTCCGGGGTCGATGC
CAAGGGCGACGACACGCCGGTAGTGAAGTTCGTCAACAAGGTGCTGGTGGATGCGATCCGGCGGGGAGCCTCCGACATCC
ATTTCGAGCCCTATGAAGACGACTACCGGGTGCGGCTGCGGATCGATGGGCTGCTGAAGAACGTGGCCAAGGCGCCGGTG
AAGTTGAGCCAGCGTATCGCGGCACGCCTGAAGGTGATGTCGCAGCTGGATATCGCCGAGAAGCGGGTGCCGCAGGACGG
GCGCATCAAGCTCAACCTGTCCAAGACCAAGCAGATCGACTTCCGCGTGAGTACCTTGCCGACATTGTTCGGCGAAAAGG
TGGTGTTGCGTATCCTGGACGGCAGCGCGGCCAAGCTGGGCATCGAGAAGCTGGGCTACGAGGCGGACCAGCAGAAGCTG
TTCCTGGAGGCGATCCACAAACCCTACGGCATGGTGCTGGTGACCGGGCCGACTGGTTCGGGCAAGACGGTGTCGCTGTA
TACGGCACTGGGTATCCTCAACGACGAGACGCGCAACATCTCCACCGCCGAGGACCCGGTAGAAATCCGCCTGCCTGGCG
TCAATCAGGTGCAGCAGAACAACAAGCGCGGCATGACTTTCGCCGCGGCGCTGCGCTCGTTCCTGCGCCAGGACCCGGAC
ATCATCATGGTCGGCGAAATCCGCGACCTGGAGACGGCCGAGATTGCGATCAAGGCCGCGCAGACCGGTCACATGGTGCT
GTCCACGCTGCATACCAACGATGCGCCGCAGACCATCGCGCGTCTGATGAACATGGGCATCGCGCCGTACAACATCACCT
CGTCGGTGACCCTGGTGATCGCGCAGCGTCTGGCGCGGCGCCTGTGCAACAACTGCAAGCGCAAATCGACCTTGCCCGAG
CATGCGTTGCTGGCCGAAGGCTTCACGCCTGCGCAGATCGCTGCCGGGATCGAGCTGTATGAGGCGGTCGGTTGCGACGA
GTGCACCGAAGGCTACAAGGGCCGTACCGGTATCTACCAGGTGATGCCGATGACCGACGAGATCGGCGCGATCGTGCTGG
AAGGCGGTAACGCGATGCAGATCGCCGAGGCGGCGCAGAGTATCGGCATCCGCGACCTGCGCCAGTCAGCGTTGGTCAAG
GCCGCGCACGGGGTGACCAGCCTGGCCGAGATCAATCGGGTGACAAAGGACTAA

Domains

Predicted by InterproScan.

Type II/IV secretion system protein (T2SSE)

(200-467)

Type II secretion system (T2SS), protein E, N-terminal domain (MshEN)

(48-152)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  pilB Acinetobacter baumannii D1279779

55.458

98.44

0.546
  pilB Acinetobacter baylyi ADP1

55.282

98.44

0.544
  pilB Legionella pneumophila strain ERS1305867

52.548

98.614

0.518
  pilB Vibrio cholerae strain A1552

48.966

100

0.492
  pilF Neisseria gonorrhoeae MS11

48.768

98.44

0.48
  pilB Vibrio parahaemolyticus RIMD 2210633

46.964

97.054

0.456
  pilB Vibrio campbellii strain DS40M4

46.046

98.614

0.454
  pilB/pilB1 Synechocystis sp. PCC 6803

36.125

100

0.381
  pilF Thermus thermophilus HB27

40.192

90.121

0.362

Multiple sequence alignment