Detailed information    

insolico Bioinformatically predicted

Overview

Name   pilE   Type   Machinery gene
Locus tag   ACNANZ_RS18765 Genome accession   NZ_CP181370
Coordinates   3949802..3950326 (-) Length   174 a.a.
NCBI ID   WP_201396210.1    Uniprot ID   -
Organism   Alicycliphilus denitrificans strain A12     
Function   assembly of type IV pilus (predicted from homology)   
DNA binding and uptake

Genomic Context

Location: 3944802..3955326
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  ACNANZ_RS18745 (ACNANZ_18745) - 3945789..3946736 (-) 948 WP_236598406.1 Bug family tripartite tricarboxylate transporter substrate binding protein -
  ACNANZ_RS18750 (ACNANZ_18750) - 3946927..3947346 (+) 420 WP_416400620.1 hypothetical protein -
  ACNANZ_RS18755 (ACNANZ_18755) miaB 3947412..3948758 (+) 1347 WP_416400621.1 tRNA (N6-isopentenyl adenosine(37)-C2)-methylthiotransferase MiaB -
  ACNANZ_RS18760 (ACNANZ_18760) - 3948950..3949711 (-) 762 WP_201396209.1 hypothetical protein -
  ACNANZ_RS18765 (ACNANZ_18765) pilE 3949802..3950326 (-) 525 WP_201396210.1 pilin Machinery gene
  ACNANZ_RS18770 (ACNANZ_18770) - 3950550..3951098 (+) 549 WP_416400622.1 hypothetical protein -
  ACNANZ_RS18775 (ACNANZ_18775) vapB 3951286..3951519 (+) 234 WP_416400623.1 type II toxin-antitoxin system VapB family antitoxin -
  ACNANZ_RS18780 (ACNANZ_18780) vapC 3951519..3951920 (+) 402 WP_236598242.1 type II toxin-antitoxin system tRNA(fMet)-specific endonuclease VapC -
  ACNANZ_RS18785 (ACNANZ_18785) ffh 3951994..3953373 (-) 1380 WP_013722921.1 signal recognition particle protein -
  ACNANZ_RS18790 (ACNANZ_18790) - 3953463..3954260 (+) 798 WP_094438286.1 cytochrome C assembly family protein -
  ACNANZ_RS18795 (ACNANZ_18795) - 3954257..3954484 (+) 228 WP_201396214.1 PP0621 family protein -

Sequence

Protein


Download         Length: 174 a.a.        Molecular weight: 18012.64 Da        Isoelectric Point: 8.9716

>NTDB_id=1099881 ACNANZ_RS18765 WP_201396210.1 3949802..3950326(-) (pilE) [Alicycliphilus denitrificans strain A12]
MKANLQKGFTLIELMIVVAIIGILAAVALPAYQDYTVRSKVSEVIIAGTSPKSLISEAFQSDGLVGAQNAAKEYNLRAAT
EKSSKYVEGIAIDEATAVITVTTKNNASAGLPTDATNKTIVFSPNVNKLALATGVTGAIDWACTSATDTTATTRGLGSKA
KGTLPAKYAPSECR

Nucleotide


Download         Length: 525 bp        

>NTDB_id=1099881 ACNANZ_RS18765 WP_201396210.1 3949802..3950326(-) (pilE) [Alicycliphilus denitrificans strain A12]
ATGAAGGCGAATCTGCAAAAGGGTTTCACCCTGATCGAACTGATGATCGTGGTGGCGATCATTGGTATTTTGGCGGCCGT
GGCGCTTCCAGCCTACCAAGACTATACGGTTCGTTCTAAGGTGTCTGAAGTAATCATCGCTGGCACTTCACCAAAGAGCC
TGATCAGCGAGGCATTCCAGAGTGATGGTCTGGTAGGGGCTCAGAATGCCGCAAAAGAATACAACTTGCGCGCTGCGACT
GAAAAGTCATCTAAATATGTTGAGGGCATTGCAATCGATGAAGCCACTGCAGTTATTACTGTCACAACTAAAAACAACGC
TTCTGCAGGACTTCCAACGGATGCGACAAATAAAACAATCGTATTTTCGCCGAATGTTAATAAGCTGGCGCTTGCTACCG
GCGTGACAGGTGCAATTGATTGGGCATGTACCTCGGCGACGGATACGACCGCAACGACTCGTGGTTTGGGTAGCAAGGCG
AAGGGTACTTTGCCCGCGAAGTATGCACCATCCGAATGCCGTTGA

Domains

Predicted by InterproScan.

Pilin (bacterial filament) (Pilin)

(37-174)

Prokaryotic N-terminal methylation motif (N_methyl)

(5-29)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  pilE Neisseria elongata subsp. glycolytica ATCC 29315

56.614

100

0.615
  pilA2 Legionella pneumophila str. Paris

42.012

97.126

0.408
  pilA2 Legionella pneumophila strain ERS1305867

41.42

97.126

0.402
  comP Acinetobacter baylyi ADP1

37.989

100

0.391
  pilA Ralstonia pseudosolanacearum GMI1000

36.872

100

0.379
  pilE Neisseria gonorrhoeae MS11

37.143

100

0.374
  pilE Neisseria gonorrhoeae strain FA1090

37.427

98.276

0.368

Multiple sequence alignment