Detailed information    

insolico Bioinformatically predicted

Overview

Name   pilB   Type   Machinery gene
Locus tag   H9T68_RS04825 Genome accession   NZ_CP181346
Coordinates   1048719..1050461 (-) Length   580 a.a.
NCBI ID   WP_280187382.1    Uniprot ID   -
Organism   Delftia sp. PS-11     
Function   power the assembly of type IV pilus (predicted from homology)   
DNA binding and uptake

Genomic Context

Location: 1043719..1055461
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  H9T68_RS04795 (H9T68_004790) mutT 1044405..1044851 (+) 447 WP_280187528.1 8-oxo-dGTP diphosphatase MutT -
  H9T68_RS04800 (H9T68_004795) - 1044870..1045079 (-) 210 WP_280187388.1 DNA gyrase inhibitor YacG -
  H9T68_RS04805 (H9T68_004800) zapD 1045085..1045840 (-) 756 WP_280187387.1 cell division protein ZapD -
  H9T68_RS04810 (H9T68_004805) coaE 1045929..1046564 (-) 636 WP_280187385.1 dephospho-CoA kinase -
  H9T68_RS04815 (H9T68_004810) pilD 1046576..1047451 (-) 876 WP_280187384.1 prepilin peptidase Machinery gene
  H9T68_RS04820 (H9T68_004815) pilC 1047451..1048668 (-) 1218 WP_280187383.1 type II secretion system F family protein Machinery gene
  H9T68_RS04825 (H9T68_004820) pilB 1048719..1050461 (-) 1743 WP_280187382.1 type IV-A pilus assembly ATPase PilB Machinery gene
  H9T68_RS04835 (H9T68_004830) - 1050812..1051741 (-) 930 WP_280187526.1 polyprenyl synthetase family protein -
  H9T68_RS04840 (H9T68_004835) rplU 1051970..1052281 (+) 312 WP_280187380.1 50S ribosomal protein L21 -
  H9T68_RS04845 (H9T68_004840) rpmA 1052297..1052554 (+) 258 WP_012207283.1 50S ribosomal protein L27 -
  H9T68_RS04850 (H9T68_004845) cgtA 1052722..1053837 (+) 1116 WP_280187378.1 Obg family GTPase CgtA -
  H9T68_RS04855 (H9T68_004850) proB 1053916..1055064 (+) 1149 WP_280187377.1 glutamate 5-kinase -

Sequence

Protein


Download         Length: 580 a.a.        Molecular weight: 62792.95 Da        Isoelectric Point: 5.2490

>NTDB_id=1099806 H9T68_RS04825 WP_280187382.1 1048719..1050461(-) (pilB) [Delftia sp. PS-11]
MAAADSSPKTASAAISLPGIGRALISAGKASEAAVEAAAKKAASKRSSFIAELCGTGEISMLEVAETVSAMFSAPLLDLD
AIDPLRLPQNVLDAKISTTYRVLALSKRGNRLTVATADPTQQEAAEQIKFTTQLGVDWVVVEADKLDRVIAQLNKSVSEA
TESAGGMIDFEFGDLAPETPPEEKENTAASDVEDAPIVKFLHKMLLDAFNMRASDLHFEPYEHQYRVRFRIDGELREIAS
PPIAIKEKLASRIKVISRLDISEKRIPQDGRMKLKVGPDRVIDFRVSTLPTLFGEKIVIRILDPSSAKMGIDALGYEPEE
KQRLLDAIGRPYGMILVTGPTGSGKTVSLYTCLNLLNQPGVNIATAEDPSEINLPGVNQVNVNEKAGLTFAAALKAFLRQ
DPDIIMVGEIRDLETADISIKAAQTGHLVLSTLHTNDAPTTLTRMRNMGIAPFNIASSVILITAQRLARRLCPQCKQPAD
IPREALLDAGYNEADLDGSWVTYKPVGCSACNNGYKGRVGIYQVMPVSEETQRIILRDGSALEIAEQARREGVRSLRESG
LHKARLGLTSLEEVLAVTNE

Nucleotide


Download         Length: 1743 bp        

>NTDB_id=1099806 H9T68_RS04825 WP_280187382.1 1048719..1050461(-) (pilB) [Delftia sp. PS-11]
ATGGCAGCTGCTGACTCCTCGCCCAAGACTGCTTCTGCGGCGATATCGCTCCCGGGTATAGGGCGAGCACTGATTTCCGC
AGGCAAGGCATCGGAGGCTGCCGTGGAGGCGGCAGCCAAGAAAGCGGCCTCCAAGCGCAGTTCGTTCATTGCCGAATTGT
GCGGCACTGGCGAAATTTCCATGCTGGAAGTGGCGGAGACGGTGTCTGCCATGTTCAGCGCGCCCTTGCTCGATCTGGAC
GCCATCGATCCGCTGCGCCTGCCCCAGAATGTGCTGGATGCCAAGATCTCCACCACCTACCGCGTTCTGGCGCTGAGCAA
ACGCGGCAACCGGCTCACGGTGGCCACGGCCGACCCTACGCAGCAGGAGGCTGCGGAGCAGATCAAGTTCACCACCCAGC
TGGGCGTGGACTGGGTCGTCGTGGAAGCCGACAAGCTGGACCGGGTGATTGCGCAGCTCAACAAGAGTGTCAGCGAAGCC
ACGGAGAGCGCTGGCGGCATGATCGACTTCGAGTTTGGCGACCTTGCGCCCGAGACGCCTCCGGAGGAGAAGGAGAACAC
GGCAGCCTCCGATGTCGAAGATGCACCGATTGTCAAGTTCCTGCACAAGATGCTGCTCGACGCCTTCAACATGCGCGCGT
CGGACTTGCACTTCGAACCCTACGAGCACCAGTACCGCGTGCGCTTTCGCATCGACGGAGAGTTGCGCGAGATTGCTTCG
CCCCCGATCGCCATCAAGGAAAAGCTGGCTTCGCGCATCAAGGTGATTTCGCGCCTGGACATCTCGGAAAAGCGGATTCC
CCAGGATGGCCGGATGAAGCTCAAGGTCGGTCCCGACCGCGTCATCGACTTTCGCGTGAGCACGCTGCCCACCCTGTTCG
GCGAGAAGATCGTGATCCGTATCCTGGACCCCAGTTCGGCCAAGATGGGCATCGATGCACTGGGCTACGAGCCGGAAGAA
AAACAGCGCCTGCTCGATGCGATCGGCAGGCCTTACGGCATGATTCTGGTCACGGGCCCCACAGGCTCGGGCAAGACCGT
GTCGCTGTACACCTGCCTGAATCTGCTGAACCAGCCCGGGGTGAATATTGCGACGGCAGAGGACCCTTCGGAAATCAATC
TGCCGGGCGTCAATCAGGTCAATGTCAACGAAAAGGCAGGGCTGACTTTTGCTGCGGCACTCAAGGCGTTTCTGCGCCAG
GATCCGGACATCATCATGGTCGGCGAAATCCGCGACCTTGAAACCGCCGACATCTCGATCAAGGCCGCCCAGACAGGCCA
CCTGGTGCTGTCCACGCTGCACACCAACGATGCCCCCACCACGCTGACGCGCATGCGCAACATGGGGATCGCGCCCTTCA
ACATCGCATCCAGCGTGATCCTCATCACGGCCCAGCGACTGGCACGGCGCCTGTGTCCGCAGTGCAAGCAGCCTGCTGAC
ATTCCTCGCGAAGCGCTGTTGGATGCAGGCTACAACGAGGCGGATCTCGACGGAAGCTGGGTGACCTACAAGCCGGTCGG
CTGCTCCGCCTGCAACAATGGCTACAAGGGCCGCGTCGGCATCTACCAAGTCATGCCCGTCAGCGAAGAGACCCAGCGCA
TCATCCTGCGCGATGGAAGCGCATTGGAAATTGCGGAGCAGGCACGCCGTGAAGGCGTGCGCTCGCTGCGCGAATCGGGA
CTGCACAAAGCCAGACTGGGACTGACCTCACTCGAAGAAGTGCTGGCCGTCACCAATGAATAA

Domains

Predicted by InterproScan.

Type II/IV secretion system protein (T2SSE)

(203-470)

Type II secretion system (T2SS), protein E, N-terminal domain (MshEN)

(63-156)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  pilB Acinetobacter baumannii D1279779

54.296

100

0.545
  pilB Acinetobacter baylyi ADP1

53.345

100

0.536
  pilF Neisseria gonorrhoeae MS11

54.426

91.552

0.498
  pilB Legionella pneumophila strain ERS1305867

49.477

98.966

0.49
  pilB Vibrio cholerae strain A1552

48.35

88.793

0.429
  pilB Vibrio campbellii strain DS40M4

44.544

96.379

0.429
  pilB Vibrio parahaemolyticus RIMD 2210633

46.958

90.69

0.426
  pilF Thermus thermophilus HB27

39.719

98.103

0.39
  pilB Deinococcus radiodurans R1 = ATCC 13939 = DSM 20539

39.054

98.448

0.384

Multiple sequence alignment