Detailed information    

insolico Bioinformatically predicted

Overview

Name   pilQ   Type   Machinery gene
Locus tag   ACGHAD_RS14870 Genome accession   NZ_AP031500
Coordinates   3514334..3516448 (-) Length   704 a.a.
NCBI ID   WP_382417766.1    Uniprot ID   -
Organism   Gilvimarinus japonicus strain 12-2     
Function   assembly of type IV pilus (predicted from homology)   
DNA binding and uptake

Genomic Context

Location: 3509334..3521448
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  ACGHAD_RS14855 (R50076_29620) - 3511087..3512613 (-) 1527 WP_382417769.1 SPOR domain-containing protein -
  ACGHAD_RS14860 (R50076_29630) aroB 3512632..3513714 (-) 1083 WP_382417768.1 3-dehydroquinate synthase -
  ACGHAD_RS14865 (R50076_29640) aroK 3513823..3514329 (-) 507 WP_339615669.1 shikimate kinase AroK -
  ACGHAD_RS14870 (R50076_29650) pilQ 3514334..3516448 (-) 2115 WP_382417766.1 type IV pilus secretin PilQ Machinery gene
  ACGHAD_RS14875 (R50076_29660) - 3516485..3517024 (-) 540 WP_382417765.1 pilus assembly protein PilP -
  ACGHAD_RS14880 (R50076_29670) - 3517017..3517631 (-) 615 WP_382417763.1 type 4a pilus biogenesis protein PilO -
  ACGHAD_RS14885 (R50076_29680) - 3517631..3518215 (-) 585 WP_339615665.1 PilN domain-containing protein -
  ACGHAD_RS14890 (R50076_29690) pilM 3518215..3519282 (-) 1068 WP_339615664.1 pilus assembly protein PilM Machinery gene

Sequence

Protein


Download         Length: 704 a.a.        Molecular weight: 76457.35 Da        Isoelectric Point: 4.3412

>NTDB_id=109966 ACGHAD_RS14870 WP_382417766.1 3514334..3516448(-) (pilQ) [Gilvimarinus japonicus strain 12-2]
MRKTVALVLVCLLSPFAGAASLDDIRFAELPDQRFEVRLSFDEAPPEPQGYTIESPARIVLDFPEVESELKERRFPLAFD
NGKSALVLTSGGRTRLILNLENLSTYSSRIDGNAYVIEVGGTGSRQVAARKPVADSAASTQPRTSAERAISGIDFRRGDA
GEGRIIVDLSTDRISADMRDTADGLRVTFANTFLPGELRRRLDVLDFATPVAIISASSDSGDVVLDVSANGDYDYLAYQT
DNQYVISVKPLTPAEKAEQERLFEYTGSRLSFNFQDIEVRAVLQIIADFTDLNLVASDTVQGRITLRLDNVPWDQALDMV
LKTKGLDKRQVGNVLMVAPASEIAERERQELSTKRQLEELAPLRTEYIRVRYASAKEIFALFQGDGAGDAGGSDGPASRA
TGSILSGRGQAVVDERTNSIIVTDTAERIDALKRLVSEIDIPIRQVMIEARLVIANKDFREELGVRWGGVGASLSSDSLL
EVGGSTSALDSSPGSPADFIYGGGSADLNENMMVDLGVASPVGSIAFGLLTDNAYLDLELSALEYNGYAEIVSQPKVITG
DKQRATISTGSEIPYQEASASGATAASFKEVVLELDVTPQITPDNNIIMELAIKKDLLAEFNQEFGVPIIDITRLNTKVL
VSDGQTIVLGGIFEMQQLNGVDKVPFLGDIPYLGRLFRRDVKTSEKSELLIFITPRIMAESLTE

Nucleotide


Download         Length: 2115 bp        

>NTDB_id=109966 ACGHAD_RS14870 WP_382417766.1 3514334..3516448(-) (pilQ) [Gilvimarinus japonicus strain 12-2]
ATGAGAAAAACAGTCGCGTTAGTCTTGGTCTGTCTGCTGTCTCCCTTTGCGGGGGCCGCGAGCCTTGATGACATCCGGTT
TGCGGAGCTGCCTGATCAGCGATTCGAGGTGCGCTTGAGCTTTGATGAAGCCCCTCCGGAGCCTCAGGGTTACACGATTG
AGTCGCCAGCGCGCATCGTATTGGATTTCCCGGAGGTCGAAAGCGAGCTGAAAGAACGGCGATTCCCGTTGGCGTTTGAT
AATGGCAAAAGCGCTTTAGTACTGACCTCGGGTGGCCGTACCCGATTGATCTTGAATCTTGAAAACCTGAGTACCTATAG
TTCTCGCATTGATGGCAACGCTTATGTCATTGAGGTAGGCGGTACTGGCTCTCGTCAAGTGGCTGCACGTAAGCCCGTGG
CCGATAGTGCCGCGAGCACTCAACCGCGTACAAGCGCAGAGCGCGCCATAAGCGGCATTGACTTCCGGCGGGGGGATGCC
GGCGAGGGTCGTATTATTGTTGATTTGTCCACTGATCGTATCAGTGCCGATATGCGCGATACGGCCGACGGTTTGCGAGT
CACTTTTGCTAATACCTTTTTACCTGGTGAGTTACGTCGCCGCTTAGATGTGCTGGATTTTGCCACGCCCGTGGCGATTA
TTTCGGCTAGCAGCGACAGTGGCGATGTGGTGCTGGATGTGTCGGCCAATGGTGATTACGATTATTTGGCCTATCAAACG
GACAATCAATATGTCATTAGTGTTAAACCCCTGACCCCAGCGGAAAAAGCCGAGCAAGAGCGTCTCTTTGAATATACTGG
CTCGCGCTTGTCGTTCAACTTTCAGGATATTGAGGTGCGAGCGGTACTGCAGATCATCGCGGATTTTACCGATTTGAACT
TAGTTGCCTCTGATACTGTGCAAGGGCGTATTACCCTTCGTTTGGATAATGTGCCTTGGGATCAAGCGCTGGATATGGTG
TTAAAGACCAAGGGTCTGGACAAGCGTCAGGTGGGTAATGTGTTGATGGTGGCTCCCGCATCGGAGATTGCCGAGCGCGA
GCGCCAGGAGCTTTCAACCAAGCGCCAGCTAGAAGAGCTGGCGCCGCTGCGTACCGAGTATATTCGTGTGCGTTATGCCA
GTGCCAAAGAGATCTTTGCACTGTTCCAAGGAGATGGTGCTGGCGACGCGGGCGGTTCAGATGGACCTGCCAGTCGTGCT
ACAGGTAGTATCTTGTCGGGACGTGGCCAAGCTGTAGTCGATGAGAGAACTAACTCGATAATTGTGACTGATACGGCTGA
GCGTATTGATGCGCTTAAACGCTTGGTGAGCGAAATCGATATCCCTATTCGTCAGGTGATGATTGAGGCGCGATTGGTTA
TTGCCAATAAGGACTTCCGCGAAGAGCTGGGCGTTCGTTGGGGTGGCGTAGGTGCCAGTTTGTCGTCTGACTCGCTCCTC
GAGGTGGGTGGCTCTACTAGCGCACTGGATTCTTCCCCTGGCTCGCCTGCCGACTTTATCTATGGTGGTGGTTCTGCTGA
TCTTAACGAGAATATGATGGTCGATTTAGGGGTTGCCAGCCCTGTAGGCAGTATTGCGTTTGGCCTGCTAACTGATAACG
CCTACTTGGATCTTGAGTTGTCGGCATTAGAGTACAACGGTTATGCTGAAATCGTGTCGCAACCTAAGGTTATTACCGGC
GATAAACAGCGCGCTACCATCTCTACCGGTAGTGAAATTCCCTACCAAGAGGCCTCTGCCAGTGGTGCAACGGCGGCATC
CTTTAAAGAGGTGGTATTGGAGCTGGATGTAACGCCCCAGATTACGCCCGACAACAACATTATTATGGAGCTGGCTATAA
AGAAAGATCTGTTAGCCGAGTTTAACCAAGAGTTTGGTGTGCCTATTATTGATATTACGCGCTTAAATACTAAGGTGCTG
GTGTCTGATGGGCAGACCATTGTTCTCGGTGGCATCTTTGAAATGCAGCAGTTAAACGGTGTGGATAAAGTCCCGTTTTT
GGGTGATATCCCTTATCTTGGGCGCTTGTTCCGCCGCGATGTTAAAACATCGGAGAAAAGTGAGTTGTTGATTTTCATTA
CCCCGCGCATTATGGCTGAGAGCTTAACCGAATAA

Domains

Predicted by InterproScan.

Bacterial type II/III secretion system short domain (Secretin_N)

(366-445)

AMIN domain (AMIN)

(32-117)

Bacterial type II and III secretion system protein (Secretin)

(542-698)

Secretin and TonB N terminus short domain (STN)

(293-339)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  pilQ Pseudomonas aeruginosa PAK

50.495

100

0.507
  pilQ Acinetobacter baumannii D1279779

40.72

100

0.418
  pilQ Acinetobacter baumannii strain A118

40.72

100

0.418
  comQ Acinetobacter baylyi ADP1

40.506

100

0.409
  pilQ Legionella pneumophila strain Lp02

37.571

99.432

0.374
  pilQ Legionella pneumophila strain ERS1305867

37.429

99.432

0.372

Multiple sequence alignment