Detailed information    

insolico Bioinformatically predicted

Overview

Name   pilB   Type   Machinery gene
Locus tag   ACM9HH_RS03780 Genome accession   NZ_CP181321
Coordinates   826966..828390 (-) Length   474 a.a.
NCBI ID   WP_416261589.1    Uniprot ID   -
Organism   Gibbsiella quercinecans strain FRB97     
Function   assembly of type IV pilus (predicted from homology)   
DNA binding and uptake

Genomic Context

Location: 821966..833390
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  ACM9HH_RS03750 (ACM9HH_03750) mutT 822271..822675 (+) 405 WP_416261585.1 8-oxo-dGTP diphosphatase MutT -
  ACM9HH_RS03755 (ACM9HH_03755) yacG 822724..822915 (-) 192 WP_095845976.1 DNA gyrase inhibitor YacG -
  ACM9HH_RS03760 (ACM9HH_03760) zapD 822967..823719 (-) 753 WP_416261586.1 cell division protein ZapD -
  ACM9HH_RS03765 (ACM9HH_03765) coaE 823716..824339 (-) 624 WP_416261587.1 dephospho-CoA kinase -
  ACM9HH_RS03770 (ACM9HH_03770) - 824677..825720 (+) 1044 WP_095845972.1 GMP reductase -
  ACM9HH_RS03775 (ACM9HH_03775) hofC 825767..826966 (-) 1200 WP_416261588.1 protein transport protein HofC -
  ACM9HH_RS03780 (ACM9HH_03780) pilB 826966..828390 (-) 1425 WP_416261589.1 type II secretion system protein GspE Machinery gene
  ACM9HH_RS03785 (ACM9HH_03785) ppdD 828396..828836 (-) 441 WP_416261590.1 prepilin peptidase-dependent pilin -
  ACM9HH_RS03790 (ACM9HH_03790) - 829084..830445 (-) 1362 WP_416261591.1 esterase-like activity of phytase family protein -
  ACM9HH_RS03795 (ACM9HH_03795) nadC 830611..831510 (-) 900 WP_121525334.1 carboxylating nicotinate-nucleotide diphosphorylase -
  ACM9HH_RS03800 (ACM9HH_03800) ampE 831786..832640 (+) 855 WP_121525335.1 beta-lactamase regulator AmpE -

Sequence

Protein


Download         Length: 474 a.a.        Molecular weight: 52328.86 Da        Isoelectric Point: 6.5854

>NTDB_id=1099647 ACM9HH_RS03780 WP_416261589.1 826966..828390(-) (pilB) [Gibbsiella quercinecans strain FRB97]
MAISNEIHALCQRYQALAVSEDEQQLTIAVHQAIPTDLLDALRFATGKQINVQQWAAARLEQAQNTRQQQGEQETQAQDV
PSLDEDSEAPVVQFINQTLRAAIQRRASDIHFEPYPQTLRIRLRIDGVLQTVPSPPFPMAARIAARLKIMGRLDIAERRL
PQDGQLSVQLDNTRYSMRIATLPTLHGEKIVLRILQSERQELPLAQLGMSQEALNLYANALGHPQGLILVTGPTGSGKTV
TLYSGLRQLNDSQTNLCSVEDPVEIPVFGINQTQINSKAELGFSRVLRALLRQDPDVIMIGEIRDRETAEIAVKAAQTGH
LVLSTLHTNSTSETLARLAQMAIPGYLLAACLKLVIAQRLLRRLCRHCRYPLSETAAFPTALWPEPLTPWRAEGCEHCFS
GYYGRIGIYELLAITPEVQEGLIQNATAGQLAQIAQRQGQKTLLQAGLEQAAQGTTSLEEVYRVLGIAAGAETR

Nucleotide


Download         Length: 1425 bp        

>NTDB_id=1099647 ACM9HH_RS03780 WP_416261589.1 826966..828390(-) (pilB) [Gibbsiella quercinecans strain FRB97]
ATGGCAATCAGCAATGAAATCCATGCACTCTGCCAGCGCTATCAAGCGCTTGCCGTTAGCGAAGACGAACAGCAGCTCAC
CATTGCCGTGCACCAGGCGATCCCAACCGATCTTCTGGATGCATTGCGCTTCGCCACCGGTAAGCAGATTAACGTGCAGC
AATGGGCGGCGGCACGCCTGGAACAGGCGCAAAATACACGCCAGCAGCAGGGCGAGCAGGAAACGCAGGCGCAGGACGTT
CCCTCACTGGACGAAGACAGCGAAGCCCCGGTGGTGCAGTTCATCAATCAGACGCTGCGCGCCGCCATTCAACGCCGGGC
GTCCGACATCCATTTTGAGCCCTATCCGCAAACGCTGCGGATCCGCTTGCGCATCGACGGCGTGCTGCAAACCGTCCCTT
CCCCGCCGTTCCCAATGGCCGCACGCATTGCGGCACGGTTGAAGATCATGGGGCGGCTGGATATTGCCGAACGCCGCCTG
CCCCAGGATGGCCAACTCAGCGTGCAGTTGGACAACACCCGTTACTCAATGCGCATCGCCACCCTGCCCACGCTGCACGG
TGAAAAGATCGTCCTGCGGATCCTGCAGAGCGAGCGCCAGGAACTGCCGCTGGCCCAGCTCGGCATGAGCCAGGAGGCAT
TAAACCTGTATGCCAACGCACTGGGTCATCCGCAGGGGTTGATCCTGGTAACCGGGCCCACCGGCAGCGGTAAAACCGTT
ACGCTTTACAGCGGCCTGCGGCAGCTTAACGACAGCCAAACCAACCTGTGCAGCGTGGAGGATCCGGTAGAGATCCCGGT
ATTCGGCATCAACCAAACGCAGATCAACAGCAAGGCCGAGCTGGGTTTCTCCCGCGTACTGCGCGCCCTGCTGCGCCAGG
ATCCTGACGTGATCATGATCGGCGAGATCCGCGACAGGGAAACGGCCGAGATCGCGGTGAAAGCCGCGCAAACCGGCCAT
CTGGTGCTCTCAACGTTGCACACCAACTCCACCAGCGAAACGTTGGCGCGCCTGGCGCAGATGGCGATACCGGGCTACCT
GCTCGCCGCCTGCCTGAAGCTGGTGATCGCCCAGCGCCTGTTGCGCCGCCTGTGCCGCCATTGCCGTTACCCGCTAAGCG
AGACGGCCGCTTTCCCCACCGCCCTGTGGCCAGAACCGCTGACGCCATGGCGTGCCGAAGGCTGTGAGCATTGCTTTAGC
GGCTATTACGGCCGGATCGGCATTTACGAACTGCTGGCGATAACCCCGGAAGTGCAAGAGGGCCTGATACAAAATGCCAC
CGCCGGGCAGTTGGCGCAGATTGCCCAGCGGCAAGGGCAAAAAACCTTGCTGCAGGCCGGGCTGGAACAGGCGGCACAGG
GCACCACGTCGCTGGAAGAAGTGTATCGGGTACTCGGTATCGCCGCTGGCGCGGAAACGCGCTGA

Domains

Predicted by InterproScan.

Type II/IV secretion system protein (T2SSE)

(97-363)

Type II secretion system (T2SS), protein E, N-terminal domain (MshEN)

(9-68)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  pilB Vibrio cholerae strain A1552

47.401

100

0.481
  pilB Vibrio campbellii strain DS40M4

44.646

100

0.466
  pilB Vibrio parahaemolyticus RIMD 2210633

45.094

100

0.456
  pilB Acinetobacter baylyi ADP1

42.116

100

0.428
  pilB Acinetobacter baumannii D1279779

41.962

100

0.424
  pilB Legionella pneumophila strain ERS1305867

45.249

93.249

0.422
  pilB Haemophilus influenzae Rd KW20

40.812

98.734

0.403
  pilB Haemophilus influenzae 86-028NP

40.812

98.734

0.403
  pilB Deinococcus radiodurans R1 = ATCC 13939 = DSM 20539

38.046

100

0.386
  pilB Glaesserella parasuis strain SC1401

39.046

97.257

0.38
  pilF Neisseria gonorrhoeae MS11

46.134

81.857

0.378
  pilB/pilB1 Synechocystis sp. PCC 6803

40.471

89.662

0.363

Multiple sequence alignment