Detailed information    

insolico Bioinformatically predicted

Overview

Name   pilF   Type   Machinery gene
Locus tag   ACM67B_RS02075 Genome accession   NZ_CP181247
Coordinates   418891..420567 (+) Length   558 a.a.
NCBI ID   WP_416189948.1    Uniprot ID   -
Organism   Neisseria sp. CCUG17229     
Function   assembly of type IV pilus (predicted from homology)   
DNA binding and uptake

Genomic Context

Location: 413891..425567
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  ACM67B_RS02050 (ACM67B_02050) - 414278..415216 (-) 939 WP_416189945.1 alpha/beta hydrolase -
  ACM67B_RS02055 (ACM67B_02055) groES 415545..415832 (+) 288 WP_095501906.1 co-chaperone GroES -
  ACM67B_RS02060 (ACM67B_02060) groL 415905..417539 (+) 1635 WP_416189946.1 chaperonin GroEL -
  ACM67B_RS02065 (ACM67B_02065) - 417666..418232 (-) 567 WP_416189947.1 hypothetical protein -
  ACM67B_RS02075 (ACM67B_02075) pilF 418891..420567 (+) 1677 WP_416189948.1 type IV-A pilus assembly ATPase PilB Machinery gene
  ACM67B_RS02080 (ACM67B_02080) pilG 420635..421876 (+) 1242 WP_416190494.1 type II secretion system F family protein Machinery gene
  ACM67B_RS02085 (ACM67B_02085) pilD 421878..422732 (+) 855 WP_416189949.1 prepilin peptidase Machinery gene
  ACM67B_RS02090 (ACM67B_02090) coaE 422742..423350 (+) 609 WP_416189950.1 dephospho-CoA kinase -
  ACM67B_RS02095 (ACM67B_02095) yacG 423353..423538 (+) 186 WP_095501899.1 DNA gyrase inhibitor YacG -
  ACM67B_RS02100 (ACM67B_02100) ispH 423620..424588 (-) 969 WP_416189951.1 4-hydroxy-3-methylbut-2-enyl diphosphate reductase -
  ACM67B_RS02105 (ACM67B_02105) lspA 424613..425113 (-) 501 WP_416189952.1 signal peptidase II -

Sequence

Protein


Download         Length: 558 a.a.        Molecular weight: 62574.29 Da        Isoelectric Point: 6.9570

>NTDB_id=1099561 ACM67B_RS02075 WP_416189948.1 418891..420567(+) (pilF) [Neisseria sp. CCUG17229]
MSVGLLRILLHNQIIKPEQAEYYQNASKHQKMISMLFDDDIISPKALAKLMAETFDYPLLDLRYYPRSRVLADVLTEEQM
VASGCVPIFRRGRKVYLAVADPTQIQIFQKLVASSRFSVDLVVVPYDQLVFLLEWIGQRSTNLLKEISQDQESSHPSQVL
YIDNEEAEDGPIPRFIHKVLSDALSVGASDIHFEFYEQTARVRFRVDGQLREVVQPPVALRGQLASRIKVMARLDISEKR
VPQDGRIQIAFHKHGRPIDFRVSTLPTLFGEKVVTRILNSDAATLNIDQLGFEPFQKEMLLDAIQRPYGMVLVTGPTGSG
KTVSLYTCLNILNTEDVNISTAEDPAEINLPGVNQVNVNDKQGLTFAAALRSFLRQDPDIIMVGEIRDLETADIAIKAAQ
TGHMVFSTLHTNNAPATLSRMLNMGVAPFNIASSVSLIMAQRLLRRLCPSCKKPIERPPVPALKKAGFTDEDLAKNWQMY
RPVGCDNCRGKGFKGRVGVYEVMPITEEMQRVIMNNGTEVDIQKMAYQEGMVDLRRAGLLKVMQGLTSLEEVTARTND

Nucleotide


Download         Length: 1677 bp        

>NTDB_id=1099561 ACM67B_RS02075 WP_416189948.1 418891..420567(+) (pilF) [Neisseria sp. CCUG17229]
ATGAGTGTCGGACTACTGCGGATATTATTACACAATCAGATAATTAAACCTGAGCAGGCAGAATATTATCAAAATGCGTC
AAAACATCAAAAGATGATATCAATGTTGTTTGATGACGACATAATTTCGCCCAAAGCGCTGGCTAAGTTAATGGCAGAAA
CATTCGATTATCCCTTGTTGGATTTGCGCTATTATCCGCGTAGTCGTGTTTTGGCCGATGTCTTAACTGAAGAGCAGATG
GTGGCAAGTGGCTGTGTACCGATTTTCCGCCGTGGCCGTAAGGTTTATTTGGCTGTTGCGGATCCGACTCAGATTCAGAT
TTTCCAAAAGCTGGTTGCATCTTCCCGGTTTTCTGTTGATCTGGTTGTGGTGCCTTATGATCAGTTGGTTTTTTTGCTGG
AATGGATTGGCCAACGATCTACAAACTTATTAAAGGAAATCAGCCAAGATCAAGAATCTTCCCATCCTTCCCAAGTTTTG
TATATTGATAACGAGGAAGCGGAAGACGGTCCGATTCCGCGTTTCATCCATAAAGTATTGTCGGATGCTTTGAGTGTGGG
TGCTTCGGATATTCATTTCGAATTTTATGAGCAGACGGCACGGGTGCGTTTTCGTGTCGATGGGCAATTACGTGAAGTGG
TGCAGCCGCCGGTTGCATTACGCGGTCAGCTGGCCTCCCGTATTAAAGTGATGGCGCGTTTGGATATCTCTGAAAAGCGT
GTACCGCAGGATGGCCGTATTCAGATTGCCTTTCATAAACATGGCCGCCCGATTGATTTTCGTGTGAGTACCTTGCCGAC
TTTATTCGGCGAAAAGGTGGTGACGCGCATTCTGAATTCGGATGCCGCCACATTGAATATCGACCAATTGGGTTTTGAGC
CATTTCAAAAAGAAATGCTGCTTGATGCTATTCAGCGGCCTTACGGCATGGTATTGGTAACAGGTCCGACCGGTTCGGGT
AAAACTGTTTCGCTTTATACCTGCCTGAATATTTTGAACACGGAAGACGTGAATATTTCCACGGCGGAAGACCCTGCCGA
GATTAACTTGCCGGGGGTTAATCAGGTTAATGTCAATGACAAGCAGGGTTTGACCTTTGCGGCCGCACTACGCTCTTTTT
TACGTCAGGATCCGGATATCATTATGGTTGGTGAGATTCGTGACTTGGAAACGGCTGACATTGCGATTAAAGCGGCACAG
ACCGGTCATATGGTCTTTTCGACACTGCATACCAATAATGCACCGGCTACTTTGTCGCGTATGTTGAATATGGGTGTGGC
TCCGTTTAATATTGCCAGTTCGGTCAGTTTGATTATGGCACAGCGTTTGTTGCGTCGTTTGTGTCCAAGCTGTAAAAAAC
CGATTGAGCGCCCTCCGGTGCCTGCATTGAAAAAAGCCGGTTTTACTGATGAAGATTTAGCTAAAAATTGGCAAATGTAT
CGCCCTGTCGGTTGCGACAATTGTCGTGGCAAAGGCTTTAAAGGGCGGGTCGGTGTATATGAAGTGATGCCCATTACAGA
GGAAATGCAGCGTGTGATTATGAATAACGGTACCGAAGTCGATATCCAAAAAATGGCCTATCAAGAAGGTATGGTGGATT
TGCGTCGGGCCGGTTTATTGAAAGTGATGCAGGGTCTGACATCATTAGAAGAGGTAACGGCACGCACCAATGACTGA

Domains

Predicted by InterproScan.

Type II secretion system (T2SS), protein E, N-terminal domain (MshEN)

(39-134)

Type II/IV secretion system protein (T2SSE)

(178-446)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  pilF Neisseria gonorrhoeae MS11

77.46

100

0.776
  pilB Acinetobacter baylyi ADP1

51.423

100

0.518
  pilB Acinetobacter baumannii D1279779

49.734

100

0.502
  pilB Legionella pneumophila strain ERS1305867

47.97

97.133

0.466
  pilB Vibrio cholerae strain A1552

45.922

100

0.464
  pilB Vibrio parahaemolyticus RIMD 2210633

45.369

94.803

0.43
  pilB Vibrio campbellii strain DS40M4

44.633

95.161

0.425
  pilF Thermus thermophilus HB27

37.011

100

0.373

Multiple sequence alignment