Detailed information    

insolico Bioinformatically predicted

Overview

Name   pilF   Type   Machinery gene
Locus tag   ACM67E_RS10280 Genome accession   NZ_CP181244
Coordinates   2231025..2232704 (-) Length   559 a.a.
NCBI ID   WP_416191345.1    Uniprot ID   -
Organism   Neisseria sp. CCUG12390     
Function   assembly of type IV pilus (predicted from homology)   
DNA binding and uptake

Genomic Context

Location: 2226025..2237704
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  ACM67E_RS10255 (ACM67E_10255) - 2227230..2227793 (-) 564 WP_416191340.1 OmpA family protein -
  ACM67E_RS10260 (ACM67E_10260) yacG 2228049..2228234 (-) 186 WP_416191341.1 DNA gyrase inhibitor YacG -
  ACM67E_RS10265 (ACM67E_10265) coaE 2228237..2228845 (-) 609 WP_416191342.1 dephospho-CoA kinase -
  ACM67E_RS10270 (ACM67E_10270) pilD 2228847..2229710 (-) 864 WP_416191343.1 prepilin peptidase Machinery gene
  ACM67E_RS10275 (ACM67E_10275) pilG 2229712..2230953 (-) 1242 WP_416191344.1 type II secretion system F family protein Machinery gene
  ACM67E_RS10280 (ACM67E_10280) pilF 2231025..2232704 (-) 1680 WP_416191345.1 type IV-A pilus assembly ATPase PilB Machinery gene
  ACM67E_RS10290 (ACM67E_10290) - 2233367..2233933 (+) 567 WP_416191346.1 hypothetical protein -
  ACM67E_RS10295 (ACM67E_10295) - 2233979..2234455 (-) 477 WP_416191347.1 YchJ family protein -
  ACM67E_RS10300 (ACM67E_10300) - 2234611..2235498 (+) 888 WP_416191348.1 RnfABCDGE type electron transport complex subunit B -
  ACM67E_RS10305 (ACM67E_10305) - 2235751..2236080 (+) 330 WP_416191349.1 hypothetical protein -
  ACM67E_RS10310 (ACM67E_10310) gap 2236394..2237425 (+) 1032 WP_416191350.1 type I glyceraldehyde-3-phosphate dehydrogenase -

Sequence

Protein


Download         Length: 559 a.a.        Molecular weight: 62149.51 Da        Isoelectric Point: 6.2975

>NTDB_id=1099474 ACM67E_RS10280 WP_416191345.1 2231025..2232704(-) (pilF) [Neisseria sp. CCUG12390]
MSVGILRVLVQSQTITSAQAEQYHAAIKAHKDIMPMLFDDGVISPRALGELVARVFSYPLLDLRYYPRTHVLTDILTEEQ
MVSNGCVPIFRRGRKVYLAVSDPTQIQNFQKITFASGFSVDLVVVPHDQLSSLLEWIGQRSTTILKEISQEQESSQPAQA
LYIDNEEAEDGPIPRFIHKTLSDALNAGASDIHFEFYEQMARVRFRVDGQLREVVQPPVAVRGQLASRIKVMARLDISEK
RVPQDGRIQIAFHKHGRPIDFRVSTLPTLFGEKVVMRILNSDAASLNIDQLGFEPFQKEMLLEAIHRPYGMVLVTGPTGS
GKTVSLYTCLNILNTEDVNISTAEDPAEINLPGINQVNVNDKQGLTFAAALKSFLRQDPDIIMVGEIRDLETADIAIKAA
QTGHMVFSTLHTNNAPATLSRMLNMGVAPFNIASSVSLIMAQRLLRRLCPNCKKPVERPPVPALKKAGFTDEDLAGNWQM
YRPVGCDSCRGKGFKGRVGVYEVMPITEEMQRVIMNNGTEVDIENMAYQEGMVDLRRAGLLKVMQGLTSLEEVTANTND

Nucleotide


Download         Length: 1680 bp        

>NTDB_id=1099474 ACM67E_RS10280 WP_416191345.1 2231025..2232704(-) (pilF) [Neisseria sp. CCUG12390]
ATGAGTGTCGGGATATTGCGCGTTTTAGTGCAAAGTCAAACCATTACCAGTGCGCAGGCGGAGCAGTATCATGCCGCCAT
CAAAGCACATAAAGACATCATGCCGATGTTGTTTGACGACGGCGTGATTTCGCCTAGAGCGCTAGGGGAATTGGTGGCGC
GAGTATTCAGCTATCCTTTGCTTGACTTGCGCTATTATCCGCGCACGCATGTTTTGACGGATATTTTGACCGAAGAGCAA
ATGGTCAGTAACGGTTGCGTGCCGATTTTCCGCCGTGGCCGAAAGGTTTATTTGGCGGTTTCTGATCCTACGCAGATTCA
GAATTTCCAAAAAATCACGTTTGCATCAGGATTCTCGGTCGATTTGGTGGTTGTACCGCATGATCAGCTGAGTTCATTGT
TGGAGTGGATCGGTCAGCGTTCGACTACTATTCTGAAAGAGATCAGCCAAGAGCAGGAGTCTTCCCAGCCTGCCCAAGCC
TTATATATTGATAATGAAGAGGCGGAAGACGGGCCGATTCCGCGTTTTATCCACAAAACGCTGTCTGATGCGCTGAATGC
CGGCGCATCGGATATTCATTTTGAGTTTTATGAGCAAATGGCGCGTGTGCGTTTCCGTGTCGACGGCCAGTTGCGCGAAG
TGGTGCAGCCGCCGGTTGCGGTGAGGGGACAGCTCGCATCGCGTATTAAAGTAATGGCGCGTTTGGATATTTCCGAGAAA
CGCGTACCGCAGGACGGCCGTATTCAGATTGCTTTCCACAAGCACGGCCGCCCGATTGACTTCCGTGTAAGCACCCTGCC
GACTTTGTTTGGTGAGAAAGTGGTGATGCGTATCCTGAATTCGGATGCGGCATCTTTGAATATCGACCAATTGGGCTTTG
AGCCGTTTCAAAAAGAAATGCTGCTTGAAGCCATTCACCGTCCTTACGGAATGGTATTGGTAACAGGTCCCACCGGTTCG
GGTAAAACGGTTTCTCTCTATACCTGTCTGAATATTTTGAATACCGAGGATGTGAATATTTCCACGGCGGAAGACCCTGC
CGAGATTAACCTGCCCGGCATCAATCAGGTTAACGTGAACGACAAACAGGGTCTGACGTTTGCAGCAGCCTTGAAATCAT
TCCTGCGTCAGGATCCCGACATCATCATGGTGGGCGAGATTCGCGACTTGGAAACTGCCGATATTGCCATCAAGGCTGCG
CAAACCGGTCATATGGTGTTCTCTACGCTGCATACCAATAATGCGCCGGCAACCTTGTCGCGTATGCTGAATATGGGCGT
GGCGCCGTTTAATATTGCCAGTTCGGTAAGCTTGATTATGGCGCAGCGTCTTTTGCGCCGTCTTTGCCCGAACTGCAAGA
AACCGGTCGAGCGCCCTCCGGTTCCTGCGTTGAAAAAAGCCGGTTTTACTGATGAAGATTTGGCAGGTAATTGGCAGATG
TATCGTCCGGTAGGGTGTGACAGTTGCCGTGGCAAAGGCTTTAAGGGCCGTGTCGGTGTGTATGAAGTGATGCCGATTAC
CGAAGAAATGCAACGCGTCATTATGAATAACGGTACTGAAGTAGACATCGAAAATATGGCATATCAGGAAGGTATGGTTG
ATTTGCGCCGTGCAGGTTTGTTGAAAGTGATGCAGGGATTGACTTCGCTGGAAGAAGTGACGGCCAATACCAACGACTAA

Domains

Predicted by InterproScan.

Type II/IV secretion system protein (T2SSE)

(179-447)

Type II secretion system (T2SS), protein E, N-terminal domain (MshEN)

(38-138)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  pilF Neisseria gonorrhoeae MS11

78.891

100

0.789
  pilB Acinetobacter baylyi ADP1

51.224

100

0.524
  pilB Acinetobacter baumannii D1279779

51.068

100

0.513
  pilB Legionella pneumophila strain ERS1305867

47.535

100

0.483
  pilB Vibrio cholerae strain A1552

46.099

100

0.465
  pilB Vibrio parahaemolyticus RIMD 2210633

46.263

100

0.465
  pilB Vibrio campbellii strain DS40M4

44.704

99.642

0.445
  pilB Deinococcus radiodurans R1 = ATCC 13939 = DSM 20539

38.931

93.739

0.365
  pilF Thermus thermophilus HB27

38.563

94.633

0.365

Multiple sequence alignment