Detailed information    

insolico Bioinformatically predicted

Overview

Name   pilB   Type   Machinery gene
Locus tag   ACGHZ6_RS19180 Genome accession   NZ_AP031498
Coordinates   4426517..4428250 (+) Length   577 a.a.
NCBI ID   WP_390615636.1    Uniprot ID   -
Organism   Maricurvus nonylphenolicus strain KU41E     
Function   power the assembly of type IV pilus (predicted from homology)   
DNA binding and uptake

Genomic Context

Location: 4421517..4433250
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  ACGHZ6_RS19160 (R50073_38240) - 4423242..4423772 (+) 531 WP_390615632.1 TIGR02281 family clan AA aspartic protease -
  ACGHZ6_RS19165 (R50073_38250) nadC 4423864..4424721 (+) 858 WP_390615633.1 carboxylating nicotinate-nucleotide diphosphorylase -
  ACGHZ6_RS19170 (R50073_38260) - 4424767..4425513 (-) 747 WP_390615634.1 hypothetical protein -
  ACGHZ6_RS19175 (R50073_38270) - 4425593..4426183 (-) 591 WP_390615635.1 prepilin-type N-terminal cleavage/methylation domain-containing protein -
  ACGHZ6_RS19180 (R50073_38280) pilB 4426517..4428250 (+) 1734 WP_390615636.1 type IV-A pilus assembly ATPase PilB Machinery gene
  ACGHZ6_RS19185 (R50073_38290) pilC 4428269..4429480 (+) 1212 WP_390615637.1 type II secretion system F family protein Machinery gene
  ACGHZ6_RS19190 (R50073_38300) pilD 4429504..4430409 (+) 906 WP_390615638.1 prepilin peptidase Machinery gene
  ACGHZ6_RS19195 (R50073_38310) coaE 4430530..4431129 (+) 600 WP_390615639.1 dephospho-CoA kinase -
  ACGHZ6_RS19200 (R50073_38320) yacG 4431131..4431346 (+) 216 WP_390615641.1 DNA gyrase inhibitor YacG -

Sequence

Protein


Download         Length: 577 a.a.        Molecular weight: 62484.19 Da        Isoelectric Point: 4.7019

>NTDB_id=109932 ACGHZ6_RS19180 WP_390615636.1 4426517..4428250(+) (pilB) [Maricurvus nonylphenolicus strain KU41E]
MNAPATAISGLARRLVSDNLISESAAEAAIKQAKQSKLPLVFQLIEDGINARDIANAASDEFGSPILDLSSINPEAIPSG
LVDAKLIQKHHVLPLFKRGNRLFLAVSDPTNLHALDEIKFNTGLNTDAILVEGDLLSKTIDTFLNAQEESLGDALGGLDD
ESLEGLDIEAVDEDGETAEDAGSEADEAPIVKFINKVLLDAIKGGASDIHFEPYEKTYRVRFRTDGILQEMAKPPTNLAG
RIAARLKVMSQMDISERRVPQDGRIKLKITKTRAIDFRVNTLPTLFGEKIVLRILDPSSAKLGIDALGYEDDQKQLYMDA
LHQPQGMILVTGPTGSGKTVSLYTGLNILNTPERNISTAEDPVEINLEGINQVQVNPKVGLTFAEALRSFLRQDPDIVMV
GEIRDLETAEIGIKAAQTGHLVLSTLHTNSAPETLTRLLNMGVPAFNVATTVSLIIAQRLARRLCGTCKKPATDIPDDIL
SKEGFDDIGVAREELEIFHPNPEGCSQCSKGYKGRVGVYEVVRITPTISSLIMEGGNALQIAQQAKEEGFNSLRISALRK
VATGLTSLEEANRVTKD

Nucleotide


Download         Length: 1734 bp        

>NTDB_id=109932 ACGHZ6_RS19180 WP_390615636.1 4426517..4428250(+) (pilB) [Maricurvus nonylphenolicus strain KU41E]
ATGAATGCACCTGCCACAGCGATTAGCGGTCTGGCAAGGCGCCTCGTTAGCGATAATCTAATTAGCGAGTCGGCAGCTGA
AGCAGCCATTAAGCAGGCTAAACAATCCAAACTGCCTTTGGTTTTTCAACTCATTGAAGACGGCATCAATGCCAGAGATA
TTGCCAATGCCGCTTCTGATGAGTTCGGCTCTCCGATTCTGGACTTATCTTCCATCAACCCAGAAGCCATCCCATCAGGA
TTAGTAGACGCCAAATTAATTCAAAAGCACCACGTACTGCCTCTTTTTAAGCGTGGCAACAGACTCTTTCTTGCCGTCTC
AGATCCCACCAATTTGCACGCACTGGACGAAATCAAGTTCAATACTGGACTTAACACAGATGCAATTCTCGTAGAAGGGG
ACCTTCTCTCCAAAACAATTGACACCTTCCTCAATGCGCAGGAGGAATCGCTAGGTGACGCGCTGGGTGGTCTGGACGAT
GAATCCCTTGAAGGCCTGGATATCGAAGCAGTCGATGAGGACGGGGAGACAGCAGAAGATGCTGGAAGCGAAGCCGATGA
AGCACCTATTGTAAAATTCATCAATAAGGTCCTTCTCGATGCTATTAAAGGCGGGGCATCTGATATTCACTTCGAACCCT
ATGAAAAAACCTATCGAGTAAGATTTCGTACTGATGGTATTTTGCAGGAAATGGCCAAACCTCCGACCAACCTGGCAGGA
CGTATTGCCGCGCGTTTAAAAGTAATGTCTCAAATGGATATTTCTGAGCGTCGTGTACCTCAAGACGGCCGCATCAAACT
GAAAATCACTAAAACCAGAGCTATCGATTTCCGGGTAAACACCTTACCGACACTCTTTGGTGAAAAGATTGTTCTGCGAA
TTCTAGACCCCAGCAGCGCCAAACTGGGTATTGATGCACTGGGTTATGAAGACGACCAGAAACAGCTCTATATGGATGCT
CTTCACCAACCCCAGGGAATGATTTTGGTAACCGGTCCTACAGGTTCAGGTAAAACCGTTTCTCTTTATACAGGGCTGAA
TATCCTAAATACGCCAGAGCGCAACATATCTACTGCTGAAGACCCGGTAGAAATCAACCTGGAAGGGATCAACCAGGTAC
AGGTTAACCCCAAGGTCGGTCTTACTTTTGCTGAGGCTCTGCGCTCATTCCTACGTCAGGATCCAGATATTGTAATGGTT
GGTGAGATCCGGGATCTGGAAACCGCAGAAATCGGTATTAAAGCTGCTCAGACTGGACACTTGGTGCTTTCAACACTGCA
TACTAATAGCGCTCCTGAAACCCTAACCCGCTTGTTAAACATGGGAGTCCCCGCGTTTAACGTGGCAACAACTGTCAGCC
TGATTATCGCCCAACGGTTAGCTCGACGACTCTGTGGTACATGCAAAAAACCAGCTACCGATATTCCAGACGACATACTA
TCGAAAGAAGGTTTTGATGATATTGGAGTCGCGAGGGAAGAGCTTGAAATTTTCCATCCCAATCCAGAAGGTTGCTCGCA
ATGCAGCAAAGGTTATAAAGGCCGGGTCGGAGTTTATGAAGTAGTTCGTATTACACCAACGATATCCAGCCTTATAATGG
AGGGTGGAAACGCCCTACAAATTGCCCAGCAAGCTAAGGAAGAAGGCTTCAACAGCCTACGCATATCTGCTCTTCGTAAG
GTAGCTACAGGCCTAACCAGCCTTGAAGAAGCAAATCGAGTGACAAAAGATTAA

Domains

Predicted by InterproScan.

Type II/IV secretion system protein (T2SSE)

(196-463)

Type II secretion system (T2SS), protein E, N-terminal domain (MshEN)

(44-149)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  pilB Acinetobacter baumannii D1279779

61.15

99.48

0.608
  pilB Acinetobacter baylyi ADP1

59.72

98.96

0.591
  pilB Legionella pneumophila strain ERS1305867

56.217

98.96

0.556
  pilB Vibrio parahaemolyticus RIMD 2210633

51.499

98.267

0.506
  pilB Vibrio campbellii strain DS40M4

50.963

98.96

0.504
  pilB Vibrio cholerae strain A1552

51.706

96.534

0.499
  pilF Neisseria gonorrhoeae MS11

47.644

99.307

0.473
  pilB/pilB1 Synechocystis sp. PCC 6803

39.488

94.801

0.374

Multiple sequence alignment