Detailed information    

insolico Bioinformatically predicted

Overview

Name   pilQ   Type   Machinery gene
Locus tag   ACGHTT_RS15930 Genome accession   NZ_AP031497
Coordinates   3591464..3593647 (-) Length   727 a.a.
NCBI ID   WP_390591923.1    Uniprot ID   -
Organism   Simiduia litorea strain KMM 9504     
Function   assembly of type IV pilus (predicted from homology)   
DNA binding and uptake

Genomic Context

Location: 3586464..3598647
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  ACGHTT_RS15915 (R50072_31710) - 3588211..3589740 (-) 1530 WP_390591920.1 SPOR domain-containing protein -
  ACGHTT_RS15920 (R50072_31720) aroB 3589781..3590881 (-) 1101 WP_390591921.1 3-dehydroquinate synthase -
  ACGHTT_RS15925 (R50072_31730) aroK 3590952..3591467 (-) 516 WP_390591922.1 shikimate kinase AroK -
  ACGHTT_RS15930 (R50072_31740) pilQ 3591464..3593647 (-) 2184 WP_390591923.1 type IV pilus secretin PilQ Machinery gene
  ACGHTT_RS15935 (R50072_31750) - 3593653..3594180 (-) 528 WP_390591924.1 pilus assembly protein PilP -
  ACGHTT_RS15940 (R50072_31760) - 3594180..3594794 (-) 615 WP_390591926.1 type 4a pilus biogenesis protein PilO -
  ACGHTT_RS15945 (R50072_31770) - 3594797..3595363 (-) 567 WP_390591927.1 PilN domain-containing protein -
  ACGHTT_RS15950 (R50072_31780) pilM 3595363..3596433 (-) 1071 WP_390591929.1 pilus assembly protein PilM Machinery gene

Sequence

Protein


Download         Length: 727 a.a.        Molecular weight: 79270.20 Da        Isoelectric Point: 4.4631

>NTDB_id=109909 ACGHTT_RS15930 WP_390591923.1 3591464..3593647(-) (pilQ) [Simiduia litorea strain KMM 9504]
MSNLIRSLTCLGLRVVAIATLIFSAGAVQANTIQDIKFAELPGQRFEVHLQFSDAPATPDGYTIDKPARIVLDFPETDNA
LSQKKYALSFENAKDAAIVSAGGRTRLIISLDDLQPYSTRTEGNQFILAVGGTNVGDSMAKANVGSSATLSQQTSVSKSL
NEKPSNSRVSVADVDFKRNAQGAGELYFTLSDAHLEVDVDEVSSGVRVTFINTFLEQELRRRLDVTDFATPVTQVNMNYD
GRNTVVDIAAPGQFDYIAYQADNSYVISVKPLNKQELEAKNAAFAFVGDKLSLNFQDIEVRSVLQLIADFTELNLVASDT
VSGRITLRLDNVPWDQALDLVLKTKGLDKRQIGNVLMVAPAAEIAERERQELETKKQLEELAPLRTEYIRVRYANAKELF
ELFKGKRESGGSSRDFGGASGGSGGDRNSTGSLLSERGRAIVDERTNSIILTDTEEKITEFKKVIAEVDIPIRQVMIEAR
IVIANTDFREELGVRWGGLGFSNGDNGQGRFTGSLETLDYDEDDTGKIEFDLNDSMMIDLGVANPAGAITAAFVDDNTYL
ALELSALEDSGHAEVVSQPKVITGDKQKATIMSGTEIPYQEASSSGATTTSFKEAVLKLDVTPQITPDNHIIMELQIHKD
SVGEITPTGIPAIDVTQLNTKVLAADGETIVLGGIFQTEQVKGVTKVPFFGDIPYLGRAFRQDVTRENKTELLIFITPRI
MADNFAQ

Nucleotide


Download         Length: 2184 bp        

>NTDB_id=109909 ACGHTT_RS15930 WP_390591923.1 3591464..3593647(-) (pilQ) [Simiduia litorea strain KMM 9504]
ATGAGTAATTTAATAAGATCACTGACCTGTTTGGGGTTAAGAGTGGTTGCGATTGCGACCTTGATTTTTAGTGCTGGTGC
AGTTCAAGCTAATACCATTCAGGATATTAAGTTTGCTGAATTGCCTGGTCAACGTTTTGAAGTGCACTTGCAGTTTAGCG
ACGCACCCGCAACCCCTGACGGCTACACCATCGATAAACCGGCGAGAATTGTTCTCGATTTTCCGGAGACAGATAATGCG
CTGAGCCAGAAAAAGTATGCACTATCTTTTGAAAATGCTAAGGATGCAGCCATTGTTAGTGCCGGCGGGCGTACTCGCTT
AATTATTTCTCTTGATGATTTGCAGCCCTATTCAACTCGGACCGAAGGGAACCAGTTTATCCTTGCAGTTGGTGGTACTA
ATGTTGGCGACTCCATGGCTAAAGCGAACGTTGGTAGTAGTGCGACATTATCCCAGCAAACCAGTGTGTCGAAGAGTTTA
AATGAGAAACCTTCAAATAGCCGAGTCAGTGTTGCAGATGTTGATTTTAAACGAAATGCGCAAGGTGCTGGTGAATTGTA
CTTTACGCTCTCTGATGCACACCTAGAGGTGGATGTTGACGAGGTGAGCAGTGGTGTTCGAGTTACCTTTATAAACACAT
TTTTAGAGCAAGAGTTACGTCGCCGTTTGGATGTAACTGACTTCGCAACGCCGGTCACTCAAGTCAATATGAATTATGAC
GGTCGTAATACGGTGGTTGATATCGCAGCCCCCGGTCAGTTTGATTATATTGCTTACCAAGCTGACAATAGCTACGTTAT
TAGTGTTAAGCCGCTAAATAAGCAAGAGTTAGAAGCTAAGAACGCGGCCTTTGCCTTTGTTGGTGACAAGCTATCATTGA
ATTTCCAAGATATTGAAGTGCGATCGGTGTTGCAGTTAATTGCTGATTTTACTGAATTGAATCTTGTTGCTTCCGATACC
GTTAGTGGTCGCATTACTTTGCGATTAGATAATGTGCCTTGGGATCAGGCTTTGGATTTGGTATTAAAAACTAAGGGCTT
GGATAAGCGTCAAATTGGTAATGTTTTGATGGTTGCACCTGCAGCTGAAATTGCTGAACGCGAACGCCAAGAGCTTGAGA
CCAAAAAGCAACTAGAAGAGTTGGCACCTTTACGTACAGAATATATTCGCGTGCGTTACGCCAATGCTAAAGAGTTGTTT
GAGTTATTTAAGGGTAAGCGAGAAAGTGGCGGGAGTAGCAGAGATTTCGGAGGTGCCAGTGGCGGTAGTGGCGGCGACAG
AAATTCCACCGGATCACTTCTGTCTGAGCGTGGGCGCGCTATTGTTGATGAGCGCACTAATTCGATTATTTTGACTGACA
CTGAAGAAAAAATTACTGAGTTCAAGAAAGTTATTGCCGAAGTGGATATTCCTATTAGGCAAGTCATGATTGAAGCGCGC
ATTGTTATTGCCAATACCGATTTCCGCGAAGAGTTGGGTGTTCGTTGGGGTGGATTGGGTTTCAGTAATGGTGATAATGG
GCAAGGCCGGTTTACCGGCTCGTTAGAGACCTTAGATTATGATGAGGACGATACGGGCAAGATAGAGTTTGATCTGAATG
ACTCTATGATGATTGACCTCGGTGTTGCTAACCCTGCCGGTGCTATTACTGCCGCTTTTGTTGATGATAATACCTATTTG
GCGTTGGAGCTGTCTGCTCTAGAGGACTCGGGCCACGCAGAAGTAGTGTCTCAGCCAAAAGTGATTACTGGCGATAAACA
GAAAGCCACGATCATGTCGGGTACAGAAATCCCCTACCAGGAAGCTTCGTCTAGCGGTGCTACGACTACCTCCTTTAAAG
AGGCCGTCTTGAAACTGGATGTAACTCCACAGATTACGCCTGATAACCACATTATTATGGAGCTTCAGATCCATAAAGAC
AGCGTAGGTGAGATTACGCCAACGGGTATTCCGGCAATTGATGTCACTCAGTTGAATACCAAAGTACTTGCTGCGGACGG
CGAAACCATTGTGCTTGGTGGAATTTTTCAGACTGAGCAAGTTAAGGGTGTTACCAAGGTTCCTTTCTTTGGCGATATTC
CTTATTTAGGTCGCGCCTTCCGTCAGGATGTTACTAGAGAGAACAAAACCGAGTTGTTGATCTTTATTACACCTCGCATT
ATGGCGGACAACTTTGCTCAATGA

Domains

Predicted by InterproScan.

Secretin and TonB N terminus short domain (STN)

(314-360)

AMIN domain (AMIN)

(47-128)

Bacterial type II/III secretion system short domain (Secretin_N)

(387-474)

Bacterial type II and III secretion system protein (Secretin)

(566-721)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  pilQ Pseudomonas aeruginosa PAK

48.343

99.587

0.481
  comQ Acinetobacter baylyi ADP1

41.337

100

0.417
  pilQ Acinetobacter baumannii strain A118

40.841

100

0.414
  pilQ Acinetobacter baumannii D1279779

40.841

100

0.414
  pilQ Legionella pneumophila strain ERS1305867

37.571

97.387

0.366
  pilQ Legionella pneumophila strain Lp02

37.429

97.387

0.365

Multiple sequence alignment