Detailed information    

insolico Bioinformatically predicted

Overview

Name   pilB   Type   Machinery gene
Locus tag   ABXH21_RS09835 Genome accession   NZ_AP031472
Coordinates   2194414..2196120 (-) Length   568 a.a.
NCBI ID   WP_353551317.1    Uniprot ID   -
Organism   Moraxella osloensis strain NBRC 115781     
Function   power the assembly of type IV pilus (predicted from homology)   
DNA binding and uptake

Genomic Context

Location: 2189414..2201120
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  ABXH21_RS09815 (SKB0120_19210) - 2190136..2191542 (+) 1407 WP_065253798.1 nicotinate phosphoribosyltransferase -
  ABXH21_RS09820 (SKB0120_19220) coaE 2191600..2192256 (-) 657 WP_353551313.1 dephospho-CoA kinase -
  ABXH21_RS09825 (SKB0120_19230) pilD 2192253..2193128 (-) 876 WP_353551314.1 A24 family peptidase Machinery gene
  ABXH21_RS09830 (SKB0120_19240) pilC 2193134..2194357 (-) 1224 WP_353551315.1 type II secretion system F family protein Machinery gene
  ABXH21_RS09835 (SKB0120_19250) pilB 2194414..2196120 (-) 1707 WP_353551317.1 type IV-A pilus assembly ATPase PilB Machinery gene
  ABXH21_RS09840 (SKB0120_19260) tpiA 2196543..2197382 (+) 840 WP_353551319.1 triose-phosphate isomerase -
  ABXH21_RS09845 (SKB0120_19270) secG 2197473..2197793 (+) 321 WP_353551321.1 preprotein translocase subunit SecG -
  ABXH21_RS09860 (SKB0120_19280) rimP 2198344..2198853 (+) 510 WP_353551323.1 ribosome maturation factor RimP -
  ABXH21_RS09865 (SKB0120_19290) nusA 2198971..2200452 (+) 1482 WP_203172425.1 transcription termination factor NusA -

Sequence

Protein


Download         Length: 568 a.a.        Molecular weight: 62647.12 Da        Isoelectric Point: 5.3387

>NTDB_id=109709 ABXH21_RS09835 WP_353551317.1 2194414..2196120(-) (pilB) [Moraxella osloensis strain NBRC 115781]
MAASLPGFGGIASRLVHEGLISEAAMQKVLAESQQQKINLIAYVVDNKLAQADAVAWVISREFGDPLIDLDAINVNYIPK
EAVDEKIIREFNILPIFQRGSRLFVAMSDPTRVDALDALRFGTRFTVETVVAEHHKIKSLIEKIFTTTNLADFDDMEAVE
FDETRADDDNSATSHLDVNDEAPVVKFVNGMLIKAITMGASDLHFEPYEKSYRVRFRIDGVLQKMANPPTQLAGKIAARL
KVMSQMDISERRVPQDGRIKLKISKDKAIDFRVNSLPTLFGEKIVLRILDPSSAMLGIDALGYEPDQKQLFMDALAKPQG
MLLITGPTGSGKTVSLYTGLNILNTVETNISTAEDPVEINLEGINQVNVNNKVGLTFASALKSFLRQDPDIVMVGEIRDL
ETAEIAVKAAQTGHMVLSTLHTNSAPETLTRLRNMGVASFNIATSVNLVIAQRLARRLCKSCKKPTDVPKNSLLEMGFTE
EDLADPSNVIYEPVGCSECRDGYKGRVGIYEVMKVTPEIARIIMEDGNAIEIKDAALKQGFRDLRRSGILKVLKGTTSIQ
EMYRVTSE

Nucleotide


Download         Length: 1707 bp        

>NTDB_id=109709 ABXH21_RS09835 WP_353551317.1 2194414..2196120(-) (pilB) [Moraxella osloensis strain NBRC 115781]
ATGGCAGCATCACTTCCTGGTTTTGGTGGCATTGCGTCACGATTAGTGCATGAAGGACTAATTAGCGAAGCGGCAATGCA
AAAAGTATTGGCAGAATCGCAGCAGCAAAAAATAAATTTAATCGCCTATGTGGTTGATAATAAGCTTGCGCAAGCTGACG
CGGTAGCGTGGGTAATTTCGCGAGAATTTGGCGATCCCCTCATTGATTTGGATGCCATCAATGTCAACTATATTCCAAAA
GAAGCGGTTGATGAGAAAATTATTCGCGAATTTAATATTCTGCCGATTTTTCAACGAGGTAGCCGCTTATTTGTTGCTAT
GAGTGACCCAACAAGGGTAGATGCTTTAGATGCACTGCGGTTTGGTACAAGGTTTACCGTAGAAACAGTGGTTGCCGAAC
ACCACAAAATCAAATCACTCATCGAAAAAATCTTTACTACCACTAATTTAGCTGATTTTGATGATATGGAAGCGGTGGAG
TTTGATGAAACCCGCGCCGATGACGACAATAGTGCCACCAGTCATTTAGATGTCAATGATGAAGCGCCTGTCGTCAAGTT
CGTTAATGGGATGCTCATCAAAGCCATTACCATGGGCGCCTCAGACTTACACTTTGAACCCTATGAAAAATCTTATCGCG
TGCGTTTTCGTATTGATGGGGTGTTACAAAAAATGGCAAATCCACCGACGCAGCTAGCGGGTAAAATTGCTGCCCGCCTG
AAAGTCATGTCACAAATGGACATCTCTGAACGCCGCGTGCCGCAAGATGGTCGTATCAAATTAAAAATTTCCAAGGATAA
GGCGATTGATTTTCGTGTCAACTCATTGCCAACCTTATTTGGTGAAAAAATCGTACTGCGTATTTTAGACCCATCATCAG
CGATGCTAGGCATTGATGCACTCGGCTATGAGCCGGACCAAAAGCAGCTATTTATGGATGCGCTGGCAAAACCGCAAGGG
ATGTTATTGATTACCGGCCCTACTGGTTCGGGTAAAACCGTCTCCTTGTACACGGGTCTTAATATCTTAAACACAGTTGA
GACCAATATTTCTACCGCAGAAGATCCCGTTGAAATTAACCTTGAAGGCATTAATCAGGTCAACGTGAACAATAAAGTTG
GCTTGACCTTTGCCAGTGCGCTCAAATCATTCTTGCGTCAAGACCCTGATATTGTGATGGTCGGTGAGATTCGTGACTTG
GAAACGGCGGAAATTGCTGTCAAAGCTGCCCAAACAGGGCATATGGTGCTGTCAACCTTGCATACCAACTCAGCGCCAGA
AACCTTAACGCGTCTGCGGAATATGGGTGTGGCATCTTTTAACATTGCCACCTCAGTCAATTTGGTAATCGCACAGCGCT
TAGCACGTCGCCTATGTAAATCCTGCAAAAAACCGACAGATGTACCAAAAAATAGCTTGCTTGAAATGGGCTTTACTGAA
GAAGATTTGGCAGATCCCAGCAATGTCATTTATGAGCCCGTTGGCTGCTCTGAATGCCGCGATGGCTATAAAGGACGTGT
TGGTATTTATGAAGTCATGAAAGTAACACCAGAGATTGCCCGAATTATTATGGAAGACGGCAATGCTATTGAGATTAAAG
ACGCTGCTTTAAAGCAAGGGTTTAGGGATTTAAGGCGCTCTGGCATTTTAAAGGTTCTAAAAGGCACGACCAGTATCCAA
GAGATGTATCGTGTTACTAGCGAATAG

Domains

Predicted by InterproScan.

Type II secretion system (T2SS), protein E, N-terminal domain (MshEN)

(15-145)

Type II/IV secretion system protein (T2SSE)

(191-457)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  pilB Acinetobacter baumannii D1279779

67.845

99.648

0.676
  pilB Acinetobacter baylyi ADP1

65.426

99.296

0.65
  pilB Legionella pneumophila strain ERS1305867

51.767

99.648

0.516
  pilF Neisseria gonorrhoeae MS11

49.016

98.415

0.482
  pilB Vibrio cholerae strain A1552

49.094

97.183

0.477
  pilB Vibrio parahaemolyticus RIMD 2210633

47.247

99.12

0.468
  pilB Vibrio campbellii strain DS40M4

46.429

98.592

0.458
  pilB Deinococcus radiodurans R1 = ATCC 13939 = DSM 20539

40.734

91.197

0.371
  pilF Thermus thermophilus HB27

36.475

100

0.368

Multiple sequence alignment