Detailed information    

insolico Bioinformatically predicted

Overview

Name   pilB   Type   Machinery gene
Locus tag   ACMZ68_RS01585 Genome accession   NZ_CP180522
Coordinates   328543..330255 (-) Length   570 a.a.
NCBI ID   WP_415871636.1    Uniprot ID   -
Organism   Acinetobacter pittii strain AP5091     
Function   power the assembly of type IV pilus (predicted from homology)   
DNA binding and uptake

Genomic Context

Location: 323543..335255
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  ACMZ68_RS01555 (ACMZ68_01555) - 323707..324033 (+) 327 WP_002115102.1 pyrimidine/purine nucleoside phosphorylase -
  ACMZ68_RS01560 (ACMZ68_01560) rlmB 324136..324885 (+) 750 WP_005079348.1 23S rRNA (guanosine(2251)-2'-O)-methyltransferase RlmB -
  ACMZ68_RS01565 (ACMZ68_01565) - 324921..325835 (+) 915 WP_032038060.1 DMT family transporter -
  ACMZ68_RS01570 (ACMZ68_01570) coaE 325832..326428 (-) 597 WP_002115011.1 dephospho-CoA kinase -
  ACMZ68_RS01575 (ACMZ68_01575) pilD 326430..327290 (-) 861 WP_415871635.1 prepilin peptidase Machinery gene
  ACMZ68_RS01580 (ACMZ68_01580) pilC 327290..328516 (-) 1227 WP_342370950.1 type II secretion system F family protein Machinery gene
  ACMZ68_RS01585 (ACMZ68_01585) pilB 328543..330255 (-) 1713 WP_415871636.1 type IV-A pilus assembly ATPase PilB Machinery gene
  ACMZ68_RS01590 (ACMZ68_01590) tpiA 330547..331341 (+) 795 WP_206261281.1 triose-phosphate isomerase -
  ACMZ68_RS01595 (ACMZ68_01595) secG 331354..331683 (+) 330 WP_002115012.1 preprotein translocase subunit SecG -
  ACMZ68_RS01615 (ACMZ68_01615) rimP 332321..332845 (+) 525 WP_002114922.1 ribosome maturation factor RimP -
  ACMZ68_RS01620 (ACMZ68_01620) nusA 332883..334367 (+) 1485 WP_002115134.1 transcription termination factor NusA -

Sequence

Protein


Download         Length: 570 a.a.        Molecular weight: 63576.91 Da        Isoelectric Point: 5.0294

>NTDB_id=1096174 ACMZ68_RS01585 WP_415871636.1 328543..330255(-) (pilB) [Acinetobacter pittii strain AP5091]
MSVIHTSPKFSGFFRRLVEEKHVSAATMQTALDAAKRAKRDTVAYLIEEVNLSPSLLAETISIEFAEPYFDLDVYDTSQI
PKDLVDQKLVLKHRVLPLIQRGQILYVATSNPSNIEAIDAIRFNSKLMVEPVIVEHHKLEKILTQQFTEESNFEFSDEEF
DLDVDLEAPSSQEEEEETPQGDEAPIVKYINKLLIDAIRMGASDLHFEPYEKSYRVRYRVDGVLRQIANPPLQLANRLAS
RLKVMSQMDISEKRVPQDGRIKLKLSKTKAIDFRVNSLPTLFGEKLVLRILDPSSAMLGIDALGYEEDQKALFLEALDKP
QGMLLITGPTGSGKTVSLYTGLNILNTESSNISTAEDPVEINLEGINQVNVNPKVGLTFSAALKSFLRQDPDIIMVGEIR
DLETAEIAIKAAQTGHMVMSTLHTNSAPETLTRLRNMGVPSFNIATSVNLVIAQRLARRLCPQCKTPADIPKQSLLEMGF
TDQDLTHPDFQVFQPVGCTECREGYKGRVGIYEVMKVTPEISKIIMEDGNALEIAAASEKLGFNNLRRSGLKKVMQGVTS
LQEVNRVTSE

Nucleotide


Download         Length: 1713 bp        

>NTDB_id=1096174 ACMZ68_RS01585 WP_415871636.1 328543..330255(-) (pilB) [Acinetobacter pittii strain AP5091]
ATGTCAGTCATACATACATCTCCAAAATTTTCGGGGTTTTTTCGACGATTAGTCGAAGAAAAACATGTGTCGGCAGCGAC
CATGCAAACAGCATTAGATGCAGCAAAGAGAGCTAAACGGGATACAGTTGCGTACCTGATTGAAGAGGTTAATCTCTCCC
CTTCTTTATTAGCTGAAACAATTTCTATTGAATTTGCTGAACCATATTTTGATCTGGATGTTTACGATACAAGCCAAATT
CCCAAGGATTTGGTCGATCAGAAACTGGTTTTAAAACATCGGGTCTTACCTCTCATACAAAGAGGACAGATTTTATATGT
CGCGACCAGCAACCCGAGTAATATTGAAGCGATTGATGCCATTCGCTTTAATAGCAAGCTCATGGTTGAACCAGTTATTG
TCGAGCACCATAAGCTTGAGAAAATTCTGACGCAGCAATTTACCGAAGAAAGCAACTTCGAGTTTAGTGATGAAGAGTTT
GATCTTGATGTAGACCTTGAAGCTCCATCTTCACAAGAAGAGGAGGAAGAAACGCCTCAAGGCGATGAAGCACCTATTGT
TAAATATATTAATAAATTACTCATCGATGCGATTCGTATGGGAGCTTCAGACTTGCATTTTGAACCCTATGAGAAATCAT
ATCGGGTTCGTTACCGAGTCGATGGTGTATTGCGCCAGATTGCAAATCCGCCTTTACAGTTGGCTAATCGTTTAGCTTCA
CGCTTAAAAGTCATGTCCCAAATGGATATTTCCGAAAAGCGAGTCCCTCAAGATGGTCGTATCAAGCTTAAGTTATCAAA
AACTAAAGCCATCGATTTTCGTGTGAACTCTCTTCCAACCTTGTTTGGTGAAAAACTGGTTCTGCGTATTCTCGATCCAT
CTAGCGCAATGCTCGGAATTGATGCTTTAGGATATGAAGAAGATCAGAAAGCTCTTTTTTTAGAAGCACTCGATAAACCA
CAAGGTATGCTTTTAATTACAGGTCCTACCGGTTCTGGTAAAACGGTATCTTTATATACTGGCTTAAATATTTTAAATAC
TGAAAGCTCTAATATTTCCACTGCCGAAGATCCAGTCGAAATTAACCTTGAAGGCATTAATCAGGTTAACGTTAACCCAA
AAGTAGGTCTTACTTTTTCGGCCGCACTGAAATCATTTTTACGTCAAGACCCTGACATTATTATGGTCGGTGAGATTCGT
GACTTAGAAACGGCTGAAATTGCGATTAAAGCTGCACAAACCGGTCATATGGTCATGTCCACACTGCATACCAATAGTGC
GCCTGAAACACTTACTCGCCTACGTAATATGGGTGTTCCATCCTTTAATATCGCAACGTCTGTCAATCTGGTGATTGCTC
AACGTTTGGCGCGTCGGTTATGCCCACAGTGTAAAACACCTGCCGACATTCCTAAACAGAGCCTATTAGAAATGGGCTTT
ACCGACCAAGATTTAACCCATCCCGATTTTCAAGTTTTTCAACCTGTGGGTTGTACTGAGTGTCGTGAAGGCTACAAAGG
TCGAGTCGGTATTTACGAGGTGATGAAAGTTACACCTGAAATTTCCAAGATTATTATGGAAGATGGCAATGCTTTAGAAA
TCGCTGCTGCTTCTGAAAAACTAGGGTTTAATAATCTGCGTCGTTCAGGGTTAAAGAAAGTCATGCAAGGGGTTACTTCT
TTACAAGAAGTCAACCGCGTGACCAGTGAATAA

Domains

Predicted by InterproScan.

Type II/IV secretion system protein (T2SSE)

(192-459)

Type II secretion system (T2SS), protein E, N-terminal domain (MshEN)

(46-145)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  pilB Acinetobacter baumannii D1279779

94.386

100

0.944
  pilB Acinetobacter baylyi ADP1

77.895

100

0.779
  pilB Legionella pneumophila strain ERS1305867

50.97

99.474

0.507
  pilF Neisseria gonorrhoeae MS11

49.466

98.596

0.488
  pilB Vibrio parahaemolyticus RIMD 2210633

51.527

91.93

0.474
  pilB Vibrio campbellii strain DS40M4

50.867

91.053

0.463
  pilB Vibrio cholerae strain A1552

50.096

91.053

0.456
  pilB/pilB1 Synechocystis sp. PCC 6803

34.853

100

0.375

Multiple sequence alignment