Detailed information    

insolico Bioinformatically predicted

Overview

Name   pilB   Type   Machinery gene
Locus tag   ACMXYJ_RS05975 Genome accession   NZ_CP180480
Coordinates   1299079..1300776 (-) Length   565 a.a.
NCBI ID   WP_415759101.1    Uniprot ID   -
Organism   Pseudomonas sp. LT1P18     
Function   power the assembly of type IV pilus (predicted from homology)   
DNA binding and uptake

Genomic Context

Location: 1294079..1305776
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  ACMXYJ_RS05940 - 1294187..1295119 (+) 933 WP_415759096.1 LysR family transcriptional regulator -
  ACMXYJ_RS05945 - 1295196..1295885 (+) 690 WP_415759097.1 energy-coupling factor ABC transporter permease -
  ACMXYJ_RS05950 - 1295949..1296164 (+) 216 WP_033054332.1 hypothetical protein -
  ACMXYJ_RS05955 yacG 1296168..1296368 (-) 201 WP_140681081.1 DNA gyrase inhibitor YacG -
  ACMXYJ_RS05960 coaE 1296365..1296988 (-) 624 WP_415759098.1 dephospho-CoA kinase -
  ACMXYJ_RS05965 pilD 1296985..1297857 (-) 873 WP_415759099.1 prepilin peptidase Machinery gene
  ACMXYJ_RS05970 pilC 1297859..1299076 (-) 1218 WP_415759100.1 type II secretion system F family protein Machinery gene
  ACMXYJ_RS05975 pilB 1299079..1300776 (-) 1698 WP_415759101.1 type IV-A pilus assembly ATPase PilB Machinery gene
  ACMXYJ_RS05980 pilA 1301003..1301410 (+) 408 WP_415759102.1 pilin Machinery gene
  ACMXYJ_RS05985 - 1301725..1302081 (+) 357 WP_415759103.1 BON domain-containing protein -
  ACMXYJ_RS05990 - 1302274..1302570 (-) 297 WP_123367810.1 DUF2845 domain-containing protein -
  ACMXYJ_RS05995 - 1302570..1305521 (-) 2952 WP_415759104.1 DUF748 domain-containing protein -

Sequence

Protein


Download         Length: 565 a.a.        Molecular weight: 62597.75 Da        Isoelectric Point: 5.8559

>NTDB_id=1096068 ACMXYJ_RS05975 WP_415759101.1 1299079..1300776(-) (pilB) [Pseudomonas sp. LT1P18]
MNDIALSGLAKQLVLAELLTDKSAQQAFQQAQRNKISLVSYLVQNKLVKSRQVAEIASEHFGMALLDLNCLDKETQPKGL
VSEKLIRQHHALPLWRRGNKLFVGVSDPTNHQAINDIQFSTGLTTEAILVEDDKLSDAIEKFFDTQGTGLEDMADVDLDG
VDIESIDDKQDAIAGQDAEDAPVVRFVHKMLLDAIKSGSSDLHFEPYEKTYRVRMRTDGILREVAKPPIHLANRIAARLK
VMASLDISERRRPQDGRIKMRLSKSKSIDFRVNTLPTLWGEKVVIRILDPSSAQMGIDALGYEPDQKDLYMAALKQPQGM
ILVTGPTGSGKTVSLYTGLNILNTVDINISTAEDPVEINMEGINQVNVNPKQGMDFAQALRSFLRQDPDVIMVGEIRDLE
TAEIAIKAAQTGHLVLSTLHTNSAAETLTRLHNMGIPGFNIATSISLIIAQRLARKLCSHCKKPIEIPREALLKEGFPEE
RIGSFTIFEPVGCDYCNGGYKGRVGIYEVVKNTQDLQRLIMAEGNSLEIDSQMRKDGFNDLRTSGLLKAMQGITSLEEIN
RVTKD

Nucleotide


Download         Length: 1698 bp        

>NTDB_id=1096068 ACMXYJ_RS05975 WP_415759101.1 1299079..1300776(-) (pilB) [Pseudomonas sp. LT1P18]
ATGAATGACATCGCCCTTAGCGGTCTGGCCAAGCAATTGGTCCTGGCCGAGCTGCTCACTGACAAAAGCGCGCAACAGGC
GTTTCAGCAAGCCCAACGCAACAAAATCTCCCTGGTCAGTTATCTGGTGCAGAACAAACTGGTGAAGAGTCGCCAGGTCG
CCGAGATCGCTTCGGAACACTTCGGCATGGCCTTGCTCGACCTCAACTGTCTGGACAAGGAAACCCAACCCAAGGGTCTG
GTCAGCGAGAAGTTGATTCGCCAGCACCACGCCCTGCCCCTCTGGCGGCGCGGCAACAAGTTGTTCGTGGGCGTTTCCGA
CCCGACCAATCATCAGGCCATCAATGACATCCAGTTCAGTACCGGGCTGACTACCGAAGCCATCCTGGTGGAGGACGACA
AGCTCAGCGACGCCATCGAAAAATTTTTCGATACCCAAGGCACTGGCCTCGAAGACATGGCCGACGTCGACCTCGATGGC
GTGGACATCGAGTCGATCGATGACAAGCAAGACGCCATCGCCGGCCAGGATGCCGAGGACGCGCCCGTGGTGCGCTTCGT
TCACAAGATGTTGCTGGACGCGATCAAAAGCGGCTCTTCCGACCTGCATTTCGAGCCCTACGAAAAGACCTACCGAGTGC
GGATGCGCACCGACGGTATTTTGCGTGAAGTCGCCAAGCCACCGATTCACCTGGCCAATCGCATTGCCGCGCGACTGAAA
GTCATGGCCAGTCTCGACATCTCCGAACGGCGCCGCCCCCAGGACGGGCGGATCAAAATGCGCCTGTCCAAGAGCAAATC
CATCGACTTTCGGGTCAATACCCTGCCGACCCTGTGGGGCGAGAAAGTGGTGATCCGGATCCTCGACCCGTCCAGCGCTC
AAATGGGCATCGACGCCCTCGGCTATGAGCCCGACCAGAAAGACCTGTACATGGCCGCCCTCAAGCAGCCACAGGGGATG
ATTCTGGTGACCGGGCCCACCGGCTCGGGCAAGACCGTATCGCTCTACACCGGGCTGAACATTCTCAACACCGTGGACAT
CAACATCTCCACCGCCGAAGACCCGGTAGAGATCAACATGGAAGGCATCAACCAGGTCAACGTCAATCCCAAACAGGGAA
TGGACTTCGCCCAGGCGCTGCGCTCGTTTCTGCGTCAAGACCCGGACGTGATCATGGTCGGCGAGATCCGCGACCTCGAA
ACCGCCGAAATCGCCATCAAAGCCGCCCAGACCGGACACCTGGTGCTGTCGACCCTGCACACCAACAGCGCCGCCGAAAC
CCTGACTCGCCTGCATAACATGGGCATTCCGGGTTTCAACATTGCCACTTCGATCAGCCTGATCATTGCCCAGCGCCTGG
CACGCAAGCTGTGCAGCCATTGCAAGAAACCCATCGAGATCCCCCGGGAAGCGCTGCTCAAGGAAGGCTTCCCCGAGGAA
CGCATCGGCTCGTTCACGATCTTTGAACCGGTCGGTTGCGATTACTGCAACGGCGGTTACAAAGGGCGAGTCGGGATTTA
TGAAGTGGTGAAGAACACACAGGACCTGCAACGGCTGATCATGGCCGAAGGCAATTCACTGGAAATCGACAGTCAGATGC
GTAAAGACGGCTTCAACGACCTGCGCACGTCAGGCCTGCTCAAGGCCATGCAAGGCATCACCAGCCTTGAAGAAATCAAC
CGGGTCACCAAGGACTGA

Domains

Predicted by InterproScan.

Type II secretion system (T2SS), protein E, N-terminal domain (MshEN)

(42-147)

Type II/IV secretion system protein (T2SSE)

(189-456)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  pilB Acinetobacter baylyi ADP1

58.541

99.469

0.582
  pilB Acinetobacter baumannii D1279779

58.007

99.469

0.577
  pilB Legionella pneumophila strain ERS1305867

55.908

100

0.561
  pilB Vibrio cholerae strain A1552

51.327

100

0.513
  pilB Vibrio parahaemolyticus RIMD 2210633

50.991

98.23

0.501
  pilB Vibrio campbellii strain DS40M4

50.177

99.823

0.501
  pilF Neisseria gonorrhoeae MS11

49.466

99.469

0.492
  pilB Deinococcus radiodurans R1 = ATCC 13939 = DSM 20539

38.899

93.274

0.363

Multiple sequence alignment