Detailed information    

insolico Bioinformatically predicted

Overview

Name   pilQ   Type   Machinery gene
Locus tag   ACMXYH_RS03040 Genome accession   NZ_CP180479
Coordinates   646630..648720 (+) Length   696 a.a.
NCBI ID   WP_415766255.1    Uniprot ID   -
Organism   Pseudomonas sp. ZB1P45     
Function   assembly of type IV pilus (predicted from homology)   
DNA binding and uptake

Genomic Context

Location: 641630..653720
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  ACMXYH_RS03020 comM 643842..644906 (+) 1065 WP_129439160.1 pilus assembly protein PilM Machinery gene
  ACMXYH_RS03025 - 644906..645472 (+) 567 WP_134419670.1 PilN domain-containing protein -
  ACMXYH_RS03030 pilO 645469..646092 (+) 624 WP_415766252.1 type 4a pilus biogenesis protein PilO -
  ACMXYH_RS03035 - 646089..646616 (+) 528 WP_415766254.1 pilus assembly protein PilP -
  ACMXYH_RS03040 pilQ 646630..648720 (+) 2091 WP_415766255.1 type IV pilus secretin PilQ Machinery gene
  ACMXYH_RS03045 aroK 648725..649243 (+) 519 WP_010464421.1 shikimate kinase AroK -
  ACMXYH_RS03050 aroB 649395..650495 (+) 1101 WP_123366938.1 3-dehydroquinate synthase -
  ACMXYH_RS03055 - 650506..652098 (+) 1593 WP_415766256.1 SPOR domain-containing protein -

Sequence

Protein


Download         Length: 696 a.a.        Molecular weight: 75290.45 Da        Isoelectric Point: 5.5547

>NTDB_id=1096029 ACMXYH_RS03040 WP_415766255.1 646630..648720(+) (pilQ) [Pseudomonas sp. ZB1P45]
MNRIFSTFGISLWIALLSPMVLAANLKALDVAALPGDRVELKLSFDGPPPPPHGYTTESPARIALDLPGVVSQLKSKNRD
LGGGNARSATVVEAKDRTRLIINLTQLTPYNARVEGNTLFVVVGQGANQTASSPAPKTPRAATATPAPAKAYTPGGKAIR
GVDFQRGTQGEGNVVIDLSDPSIAPDIQERDGKIILGFARTQLPEPLRVRLDVKDFATPVQFVNASATGDRATISIEPSG
AFDYSTYQTDNKLTVSIRPMTVDDLQKRNTERFAYTGEKLSLNFQDIDVRSVLQLIADFTNLNLVASDTVQGGITLRLQN
VPWDQALDLVLKTKGLDKRKIGNVLLVAPADEIAARERQELESQKQIADLAPLRRELLQVNYAKAADIAKLFQSVTSAEA
KADERGSITVDERTNNIIAYQTQDRLDELRRIVAQLDIPVRQVMIEARIVEANVDYDKSLGVRWGGSLQNKGNWNTSGVS
NNGANASSTIGTPGSTSTNSPFVDLGTAANTSGLGIAFITDNVLLDLELTAMEKTGNGEIVSQPKVVTSDKETAKILKGT
EIPYQEASSSGATSVSFKEASLSLEVTPQITPDNRIIMEVKVTKDEPDYLNKVQDVPPIKKNEVNAKVLVNDGETIVIGG
VFSNTQSKVVDKVPFLGDVPYLGRLFRRDVVSEKKSELLVFLTPRIMNNQAIAVSH

Nucleotide


Download         Length: 2091 bp        

>NTDB_id=1096029 ACMXYH_RS03040 WP_415766255.1 646630..648720(+) (pilQ) [Pseudomonas sp. ZB1P45]
ATGAACAGGATTTTCTCAACCTTCGGTATTTCGCTATGGATAGCGTTGCTGTCGCCGATGGTACTAGCGGCCAACCTCAA
AGCGCTGGATGTCGCCGCGCTGCCGGGGGACCGCGTCGAGTTGAAGTTGTCGTTCGATGGGCCGCCACCGCCGCCCCACG
GTTACACCACGGAATCGCCTGCACGGATCGCGCTGGATCTGCCTGGCGTCGTCAGTCAGCTAAAAAGCAAAAATCGTGAC
CTGGGCGGCGGAAATGCCCGTAGCGCCACGGTGGTGGAAGCCAAGGACCGTACGCGACTGATCATCAATCTGACCCAGTT
GACGCCGTATAACGCACGGGTCGAAGGCAACACGCTGTTCGTGGTGGTCGGCCAAGGTGCCAACCAAACGGCCTCCAGCC
CGGCGCCCAAAACACCGCGCGCCGCGACGGCAACGCCAGCCCCGGCGAAAGCCTATACGCCAGGGGGCAAGGCCATCCGG
GGTGTGGATTTCCAGCGCGGCACTCAGGGCGAAGGCAATGTGGTCATCGATCTGTCGGATCCCTCCATCGCCCCGGACAT
TCAGGAGCGCGACGGCAAGATCATCCTCGGTTTCGCCAGGACTCAATTGCCTGAGCCGTTGCGTGTACGTCTGGACGTCA
AGGATTTCGCCACGCCGGTGCAGTTCGTCAACGCCAGCGCCACGGGCGATCGGGCCACGATCAGCATTGAGCCCAGCGGC
GCCTTCGATTATTCGACTTACCAGACCGACAACAAGCTGACCGTCAGCATCCGGCCGATGACCGTCGATGACTTGCAGAA
ACGCAACACCGAGCGTTTCGCCTACACGGGCGAAAAGCTCTCGCTGAATTTCCAGGACATCGACGTGCGTTCGGTGCTGC
AACTGATTGCCGATTTCACCAACCTCAATCTGGTGGCCAGCGACACAGTGCAGGGCGGCATCACGCTGCGTTTGCAGAAC
GTACCGTGGGATCAGGCGCTGGATCTGGTGTTGAAAACCAAGGGCCTGGACAAGCGAAAGATCGGCAACGTTTTGCTGGT
GGCGCCGGCCGATGAAATCGCTGCCCGCGAGCGCCAGGAGCTTGAGTCGCAGAAGCAGATTGCTGACCTGGCGCCCCTGC
GTCGCGAACTGTTGCAGGTCAACTACGCCAAAGCTGCCGATATCGCCAAGCTGTTCCAGTCGGTGACCAGTGCCGAGGCA
AAAGCCGACGAGCGGGGCTCGATCACGGTGGATGAGCGGACCAACAACATCATTGCCTACCAGACCCAGGATCGCCTCGA
TGAACTGCGCCGTATCGTCGCGCAACTGGATATCCCGGTGCGTCAGGTGATGATCGAGGCGCGAATCGTCGAAGCCAACG
TCGACTATGACAAAAGCCTCGGCGTGCGTTGGGGTGGTTCGTTGCAGAACAAGGGCAACTGGAACACCTCGGGCGTCAGC
AATAATGGGGCTAATGCTTCGTCGACCATCGGTACGCCTGGCAGCACCAGCACCAACTCGCCGTTCGTCGACCTGGGTAC
CGCCGCCAACACCTCGGGGCTCGGCATCGCGTTCATCACCGACAATGTGCTGCTGGATCTTGAGCTGACGGCGATGGAGA
AGACCGGCAACGGCGAAATCGTCTCGCAGCCCAAGGTGGTCACCTCCGACAAGGAAACCGCGAAAATCCTCAAGGGCACG
GAGATTCCCTATCAGGAAGCCAGCTCCAGCGGCGCTACCTCGGTGTCGTTCAAGGAGGCTTCGCTGTCTCTGGAGGTGAC
GCCGCAGATCACCCCGGACAACCGGATCATCATGGAGGTCAAGGTCACCAAGGACGAACCGGACTACCTGAACAAGGTGC
AGGATGTACCGCCGATCAAGAAAAACGAGGTCAATGCCAAGGTCCTGGTCAACGACGGTGAGACCATCGTGATTGGCGGG
GTTTTCTCAAATACTCAGAGCAAGGTTGTAGATAAGGTGCCATTTCTCGGTGATGTGCCGTATCTTGGCCGCCTTTTCCG
GCGTGACGTGGTTTCGGAGAAAAAATCCGAGCTGCTGGTATTTCTCACTCCGCGTATCATGAATAACCAGGCGATTGCTG
TGAGTCATTGA

Domains

Predicted by InterproScan.

AMIN domain (AMIN)

(37-121)

Bacterial type II/III secretion system short domain (Secretin_N)

(377-442)

Secretin and TonB N terminus short domain (STN)

(303-349)

Bacterial type II and III secretion system protein (Secretin)

(531-687)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  pilQ Pseudomonas aeruginosa PAK

73.566

100

0.756
  comQ Acinetobacter baylyi ADP1

40.625

100

0.43
  pilQ Acinetobacter baumannii strain A118

38.095

100

0.414
  pilQ Acinetobacter baumannii D1279779

38.095

100

0.414
  pilQ Legionella pneumophila strain ERS1305867

37.814

97.27

0.368
  pilQ Legionella pneumophila strain Lp02

37.666

97.27

0.366

Multiple sequence alignment