Detailed information NTG1092434

Bioinformatically predicted

Overview

• Name: pilU
• Type: Machinery gene
• Locus tag: ACKW6E_RS04905
• Genome accession: NZ_CP178915
• Coordinates: 1083864..1084994 (+)
• Length: 376 a.a.
• NCBI ID: WP_054663718.1
• Uniprot ID: A0A0R0E4U6
• Organism: Stenotrophomonas acidaminiphila strain ZCMU-Sa01
• Function: assembly of type IV pilus (predicted from homology) DNA binding and uptake

Genomic Context

Location: 1078864..1089994

Locus tag	Gene name	Coordinates (strand)	Size (bp)	Protein ID	Product	Description
ACKW6E_RS04890 (ACKW6E_04890)	-	1079544..1081499 (+)	1956	WP_054663708.1	esterase/lipase family protein	-
ACKW6E_RS04895 (ACKW6E_04895)	proC	1081588..1082427 (-)	840	WP_413975794.1	pyrroline-5-carboxylate reductase	-
ACKW6E_RS04900 (ACKW6E_04900)	pilT	1082694..1083731 (+)	1038	WP_104938226.1	type IV pilus twitching motility protein PilT	Machinery gene
ACKW6E_RS04905 (ACKW6E_04905)	pilU	1083864..1084994 (+)	1131	WP_054663718.1	PilT/PilU family type 4a pilus ATPase	Machinery gene
ACKW6E_RS04910 (ACKW6E_04910)	-	1085668..1086216 (+)	549	WP_282884665.1	DUF3617 domain-containing protein	-

Sequence

Protein

• Length: 376 a.a.
• Molecular weight: 41809.11 Da
• Isoelectric Point: 7.0672

>NTDB_id=1092434 ACKW6E_RS04905 WP_054663718.1 1083864..1084994(+) (pilU) [Stenotrophomonas acidaminiphila strain ZCMU-Sa01]
MSTIDFTSFLKLMAHQKASDLFITAGMPPSMKVHGRISPITQTPLTPQQSRDMVLNVMTPAQREEFEKTHECNFAIGLSG
VGRFRVSCFYQRNQVGMVLRRIETRIPTVEELNLPPVIKTLAMTKRGIILFVGATGTGKSTSLAAMIGYRNQNSTGHIIT
IEDPIEFVHKHEGCIITQREVGIDTDSWEAALKNTLRQAPDVIMIGEVRTREGMDHAIAFAETGHLVLATLHANNANQAM
DRIINFFPEDRRSQLLMDLSLNLKGVVAQQLVPTPDGKGRRVAMEILLGTPLVQDYIRDGEIHKLKEVMKESVQLGMKTF
DQSLFELYQAGEISYDDALRHADSQNEVRLRIKLSQGGDAKTLSQGLDGVEIAGTR

Nucleotide

• Length: 1131 bp

>NTDB_id=1092434 ACKW6E_RS04905 WP_054663718.1 1083864..1084994(+) (pilU) [Stenotrophomonas acidaminiphila strain ZCMU-Sa01]
ATGAGCACCATCGATTTCACTTCGTTCCTCAAGCTGATGGCGCACCAGAAGGCGTCGGACCTGTTCATCACCGCCGGCAT
GCCGCCGTCGATGAAGGTGCATGGCAGGATCAGCCCGATCACGCAGACGCCGCTGACCCCGCAGCAGAGCCGCGACATGG
TGCTCAACGTGATGACGCCGGCGCAGCGCGAGGAGTTCGAGAAGACCCACGAGTGCAACTTCGCCATCGGCCTGTCTGGC
GTGGGCCGCTTCCGCGTCAGCTGTTTCTACCAGCGCAACCAGGTGGGCATGGTGCTGCGCCGGATCGAGACGCGGATCCC
GACGGTGGAGGAGCTGAACCTGCCGCCGGTGATCAAGACGCTGGCGATGACCAAGCGCGGCATCATCCTGTTCGTCGGCG
CCACCGGCACCGGCAAGTCGACCTCGCTGGCGGCGATGATCGGCTACCGCAACCAGAATTCGACCGGGCACATCATCACC
ATCGAGGACCCGATCGAGTTCGTGCACAAGCACGAAGGTTGCATCATCACCCAGCGCGAAGTGGGCATCGACACCGACAG
CTGGGAAGCCGCGCTGAAGAACACCCTGCGCCAGGCGCCGGACGTGATCATGATCGGCGAGGTCCGCACCCGCGAAGGCA
TGGACCACGCCATCGCCTTCGCCGAGACCGGCCACCTGGTGCTGGCGACGCTGCATGCCAACAACGCCAACCAGGCGATG
GACCGCATCATCAACTTCTTCCCTGAAGACCGCCGCAGCCAGCTGCTGATGGACCTTTCGCTCAACCTCAAGGGCGTGGT
CGCGCAGCAGCTGGTGCCGACGCCCGATGGCAAGGGGCGCCGGGTGGCGATGGAGATCCTGCTGGGCACGCCGCTGGTGC
AGGACTACATCCGCGACGGCGAGATCCACAAGCTCAAGGAAGTGATGAAGGAATCGGTGCAGCTGGGCATGAAGACCTTC
GACCAGAGCCTGTTCGAGCTGTACCAGGCCGGCGAGATCAGCTACGACGACGCGCTGCGCCATGCCGATTCCCAGAACGA
GGTGCGCCTGCGCATCAAGCTCTCGCAGGGCGGCGATGCCAAGACCCTTTCGCAGGGGCTCGATGGCGTGGAGATCGCCG
GCACGCGCTGA

Domains

Predicted by InterproScan.

• Type II/IV secretion system protein (T2SSE) (118-272)

3D structure

• AlphaFold DB: A0A0R0E4U6

Similar proteins

Only experimentally validated proteins are listed.

Protein	Organism	Identities (%)	Coverage (%)	Ha-value
pilU	Pseudomonas stutzeri DSM 10701	68.937	97.606	0.673
pilU	Acinetobacter baylyi ADP1	64.426	94.947	0.612
pilU	Vibrio cholerae strain A1552	54.494	94.681	0.516
pilT	Deinococcus radiodurans R1 = ATCC 13939 = DSM 20539	43.137	94.947	0.41
pilT	Acinetobacter nosocomialis M2	39.535	91.489	0.362
pilT	Acinetobacter baumannii D1279779	39.535	91.489	0.362
pilT	Acinetobacter baumannii strain A118	39.535	91.489	0.362