Detailed information NTG1090857

Bioinformatically predicted

Overview

• Name: comEA
• Type: Machinery gene
• Locus tag: ACL6ER_RS12140
• Genome accession: NZ_CP178717
• Coordinates: 2463058..2463675 (-)
• Length: 205 a.a.
• NCBI ID: WP_060399060.1
• Uniprot ID: A0A9Q4HTS0
• Organism: Bacillus inaquosorum strain SY1167
• Function: dsDNA binding to the cell surface (predicted from homology) DNA binding and uptake

Genomic Context

Location: 2458058..2468675

Locus tag	Gene name	Coordinates (strand)	Size (bp)	Protein ID	Product	Description
ACL6ER_RS12110 (ACL6ER_12110)	rpsT	2458357..2458623 (+)	267	WP_003229989.1	30S ribosomal protein S20	-
ACL6ER_RS12115 (ACL6ER_12115)	holA	2458638..2459681 (-)	1044	WP_268332604.1	DNA polymerase III subunit delta	-
ACL6ER_RS12120 (ACL6ER_12120)	-	2459721..2459870 (-)	150	WP_003229985.1	hypothetical protein	-
ACL6ER_RS12125 (ACL6ER_12125)	-	2459911..2460045 (+)	135	WP_003229983.1	YqzM family protein	-
ACL6ER_RS12130 (ACL6ER_12130)	-	2460086..2462418 (-)	2333	Protein_2381	DNA internalization-related competence protein ComEC/Rec2	-
ACL6ER_RS12135 (ACL6ER_12135)	-	2462422..2462991 (-)	570	WP_268332605.1	ComE operon protein 2	-
ACL6ER_RS12140 (ACL6ER_12140)	comEA	2463058..2463675 (-)	618	WP_060399060.1	helix-hairpin-helix domain-containing protein	Machinery gene
ACL6ER_RS12145 (ACL6ER_12145)	comER	2463759..2464580 (+)	822	WP_060399061.1	late competence protein ComER	-
ACL6ER_RS12150 (ACL6ER_12150)	-	2464646..2465389 (-)	744	WP_060399062.1	class I SAM-dependent DNA methyltransferase	-
ACL6ER_RS12155 (ACL6ER_12155)	rsfS	2465386..2465742 (-)	357	WP_003237112.1	ribosome silencing factor	-
ACL6ER_RS12160 (ACL6ER_12160)	yqeK	2465760..2466320 (-)	561	WP_003237115.1	bis(5'-nucleosyl)-tetraphosphatase (symmetrical) YqeK	-
ACL6ER_RS12165 (ACL6ER_12165)	-	2466310..2466879 (-)	570	WP_019258940.1	nicotinate-nucleotide adenylyltransferase	-
ACL6ER_RS12170 (ACL6ER_12170)	yhbY	2466891..2467181 (-)	291	WP_003226133.1	ribosome assembly RNA-binding protein YhbY	-
ACL6ER_RS12175 (ACL6ER_12175)	aroE	2467175..2468017 (-)	843	WP_119914036.1	shikimate dehydrogenase	-

Sequence

Protein

• Length: 205 a.a.
• Molecular weight: 21754.65 Da
• Isoelectric Point: 4.9529

>NTDB_id=1090857 ACL6ER_RS12140 WP_060399060.1 2463058..2463675(-) (comEA) [Bacillus inaquosorum strain SY1167]
MNWLNQYKKTIILAASAAVFIAAIFIFASGKNKGPVKQAVSTETENAEVKQEAVKDESNKTIVIDIKGAVLHPGVYEMRT
GDRVSQAIEKAGGASEQADEMQVNLAELLQDGTVVYIPKKGEETAVQQGAVGAVQSDGGKGALVNINTATLDELQGISGV
GPSKAEAIIAYREENGRFQTIEDITKVSGIGEKSFEKIKSSITVK

Nucleotide

• Length: 618 bp

>NTDB_id=1090857 ACL6ER_RS12140 WP_060399060.1 2463058..2463675(-) (comEA) [Bacillus inaquosorum strain SY1167]
ATGAATTGGTTGAATCAGTATAAAAAAACAATAATTTTAGCGGCTTCTGCGGCTGTTTTCATAGCGGCTATTTTCATCTT
TGCCAGCGGAAAAAACAAAGGGCCGGTGAAGCAGGCTGTATCAACTGAGACAGAAAATGCGGAGGTAAAGCAGGAAGCAG
TCAAAGACGAGTCAAACAAAACAATAGTGATAGACATTAAAGGTGCTGTTCTGCATCCTGGCGTTTATGAAATGCGAACA
GGGGACAGAGTATCTCAGGCAATTGAGAAAGCGGGCGGGGCCAGTGAACAAGCAGACGAAATGCAAGTGAACTTGGCGGA
GCTGCTTCAGGACGGGACAGTGGTGTACATCCCGAAAAAGGGAGAGGAGACGGCGGTGCAGCAAGGAGCCGTAGGCGCTG
TTCAAAGCGATGGAGGGAAGGGAGCGCTCGTAAATATCAATACAGCCACCTTAGACGAATTACAAGGCATCTCAGGGGTG
GGACCGTCAAAAGCTGAAGCTATTATTGCATACCGGGAGGAAAACGGGCGTTTCCAGACAATTGAAGATATCACTAAGGT
TTCAGGAATAGGTGAAAAGTCATTTGAGAAAATAAAGTCTTCCATTACAGTAAAGTGA

Domains

Predicted by InterproScan.

• Helix-hairpin-helix motif (HHH_3) (142-203)

• SLBB domain (SLBB) (63-118)

Similar proteins

Only experimentally validated proteins are listed.

Protein	Organism	Identities (%)	Coverage (%)	Ha-value
comEA	Bacillus subtilis subsp. subtilis str. 168	88.293	100	0.883
comEA	Staphylococcus aureus MW2	38.636	100	0.415
comEA	Staphylococcus aureus N315	37.273	100	0.4
comEA	Lactococcus lactis subsp. cremoris KW2	35.78	100	0.38
comEA/celA/cilE	Streptococcus pneumoniae R6	36.715	100	0.371
comEA/celA/cilE	Streptococcus pneumoniae Rx1	36.715	100	0.371
comEA/celA/cilE	Streptococcus pneumoniae D39	36.715	100	0.371