Detailed information    

insolico Bioinformatically predicted

Overview

Name   comGB/cglB   Type   Machinery gene
Locus tag   ACAM23_RS10065 Genome accession   NZ_AP031394
Coordinates   1917565..1918581 (-) Length   338 a.a.
NCBI ID   WP_073177425.1    Uniprot ID   -
Organism   Streptococcus pneumoniae strain 16P28     
Function   dsDNA binding to the cell surface; assembly of the pseudopilus (predicted from homology)   
DNA binding and uptake

Genomic Context

Location: 1912565..1923581
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  ACAM23_RS10020 - 1912649..1912780 (-) 132 WP_000768903.1 hypothetical protein -
  ACAM23_RS10025 (SPNE16P28_19440) - 1912781..1913971 (-) 1191 WP_000167759.1 acetate kinase -
  ACAM23_RS10030 (SPNE16P28_19450) comYH 1914022..1914975 (-) 954 WP_000345123.1 class I SAM-dependent methyltransferase Machinery gene
  ACAM23_RS10035 (SPNE16P28_19460) - 1915036..1915623 (-) 588 WP_000679774.1 class I SAM-dependent methyltransferase -
  ACAM23_RS10040 (SPNE16P28_19470) comGG/cglG 1915760..1916173 (-) 414 WP_000265630.1 competence type IV pilus minor pilin ComGG Machinery gene
  ACAM23_RS10045 (SPNE16P28_19480) comGF/cglF 1916151..1916612 (-) 462 WP_000250548.1 competence type IV pilus minor pilin ComGF Machinery gene
  ACAM23_RS10050 (SPNE16P28_19490) comGE/cglE 1916575..1916877 (-) 303 WP_000413380.1 competence type IV pilus minor pilin ComGE Machinery gene
  ACAM23_RS10055 (SPNE16P28_19500) comGD/cglD 1916840..1917244 (-) 405 WP_000588007.1 competence type IV pilus minor pilin ComGD Machinery gene
  ACAM23_RS10060 (SPNE16P28_19510) comGC/cglC 1917237..1917563 (-) 327 WP_000738624.1 competence type IV pilus major pilin ComGC Machinery gene
  ACAM23_RS10065 (SPNE16P28_19520) comGB/cglB 1917565..1918581 (-) 1017 WP_073177425.1 competence type IV pilus assembly protein ComGB Machinery gene
  ACAM23_RS10070 (SPNE16P28_19530) comGA/cglA/cilD 1918529..1919470 (-) 942 WP_000249559.1 competence type IV pilus ATPase ComGA Machinery gene
  ACAM23_RS10075 (SPNE16P28_19540) - 1919546..1919911 (-) 366 WP_000286415.1 DUF1033 family protein -
  ACAM23_RS10080 (SPNE16P28_19550) - 1920178..1921433 (+) 1256 WP_088804233.1 ISL3 family transposase -
  ACAM23_RS10085 (SPNE16P28_19560) - 1921482..1922540 (-) 1059 WP_000649468.1 zinc-dependent alcohol dehydrogenase family protein -

Sequence

Protein


Download         Length: 338 a.a.        Molecular weight: 38420.45 Da        Isoelectric Point: 9.4802

>NTDB_id=109002 ACAM23_RS10065 WP_073177425.1 1917565..1918581(-) (comGB/cglB) [Streptococcus pneumoniae strain 16P28]
MDISQVFRLRRKKLATAKQKNIITLFNNLFSSGFHLVETISFLDRSALLDKQCVIQMRAGLSQGKSFSEMMESLGCSSTI
VTQLSLAEVHGNLHLSLGKIEEYLDNLAKVKKKLIEVATYPLILLGFLLLIMLGLRNYLLPQLDSSNIATQIIGNLPQIF
LGMVGLVSVLALLALTFYKRSSKMSVFSILARLPFIGIFVQTYLTAYYAREWGNMISQGMELTQIFQMMQEQGSQLFKEI
GQDLAQTLKNGREFSQTIGTYPFFRKELSLIIEYGEVKSKLGSELEIYAEKTWEAFFTRVNRTMNLVQPLVFIFVALIIV
LLYAAMLMPMYQNMEVNF

Nucleotide


Download         Length: 1017 bp        

>NTDB_id=109002 ACAM23_RS10065 WP_073177425.1 1917565..1918581(-) (comGB/cglB) [Streptococcus pneumoniae strain 16P28]
ATGGACATATCACAAGTCTTCAGGCTGAGACGGAAAAAATTAGCTACAGCTAAGCAAAAAAATATCATCACCCTATTTAA
CAATCTCTTTTCTAGCGGTTTTCATCTGGTGGAGACTATCTCCTTTTTAGATAGGAGTGCTTTGTTGGACAAGCAGTGTG
TGATCCAGATGCGTGCGGGCTTGTCTCAAGGGAAATCATTCTCAGAAATGATGGAAAGTTTGGGATGTTCAAGTACCATT
GTCACTCAGTTATCCCTAGCCGAAGTTCATGGAAATCTCCACCTGAGTTTGGGAAAGATAGAAGAATATCTGGACAATCT
GGCTAAGGTCAAGAAAAAATTAATTGAAGTAGCGACCTATCCTTTGATTTTGCTGGGTTTTCTTCTCTTAATTATGCTGG
GGCTACGGAATTACCTGCTCCCACAACTGGATAGTAGCAATATTGCCACCCAAATTATCGGTAATCTGCCCCAAATTTTT
CTAGGCATGGTAGGGCTTGTTTCCGTGCTTGCCCTTTTAGCACTAACTTTTTATAAAAGAAGTTCTAAGATGAGTGTCTT
TTCTATCTTAGCACGCCTTCCCTTTATTGGAATCTTTGTGCAGACCTACTTGACAGCCTATTATGCACGTGAATGGGGGA
ATATGATTTCACAGGGAATGGAGCTGACGCAGATTTTTCAAATGATGCAGGAACAAGGTTCCCAGCTCTTTAAAGAAATC
GGTCAAGATCTGGCTCAAACCCTGAAAAATGGCCGTGAATTTTCTCAGACGATAGGAACCTATCCTTTCTTTAGGAAGGA
ATTGAGTCTCATCATAGAGTATGGGGAAGTTAAGTCCAAGCTGGGTAGTGAGTTGGAAATCTATGCTGAAAAAACTTGGG
AAGCCTTTTTTACCCGAGTCAACCGCACCATGAATTTGGTGCAGCCACTGGTTTTTATCTTTGTGGCACTGATTATCGTT
TTACTTTATGCGGCAATGCTCATGCCCATGTATCAAAATATGGAGGTAAATTTTTAA

Domains

Predicted by InterproScan.

Type II secretion system (T2SS), protein F (T2SSF)

(209-329)

Type II secretion system (T2SS), protein F (T2SSF)

(24-141)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  comGB/cglB Streptococcus pneumoniae Rx1

98.817

100

0.988
  comGB/cglB Streptococcus pneumoniae D39

98.817

100

0.988
  comGB/cglB Streptococcus pneumoniae R6

98.817

100

0.988
  comGB/cglB Streptococcus pneumoniae TIGR4

98.817

100

0.988
  comGB/cglB Streptococcus mitis SK321

94.97

100

0.95
  comGB/cglB Streptococcus mitis NCTC 12261

94.675

100

0.947
  comYB Streptococcus gordonii str. Challis substr. CH1

71.131

99.408

0.707
  comYB Streptococcus mutans UA140

57.862

94.083

0.544
  comYB Streptococcus mutans UA159

57.862

94.083

0.544
  comGB Lactococcus lactis subsp. cremoris KW2

50.898

98.817

0.503

Multiple sequence alignment