Detailed information    

insolico Bioinformatically predicted

Overview

Name   pilC   Type   Machinery gene
Locus tag   ACLRDL_RS12630 Genome accession   NZ_CP178387
Coordinates   2772351..2773577 (+) Length   408 a.a.
NCBI ID   WP_000648509.1    Uniprot ID   -
Organism   Vibrio cholerae isolate CTMA_1842     
Function   assembly of type IV pilus (predicted from homology)   
DNA binding and uptake

Genomic Context

Location: 2767351..2778577
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  ACLRDL_RS12605 (ACLRDL_12605) fldB 2767505..2768023 (+) 519 WP_000690112.1 flavodoxin FldB -
  ACLRDL_RS12610 (ACLRDL_12610) ampD 2768174..2768719 (-) 546 WP_000567319.1 1,6-anhydro-N-acetylmuramyl-L-alanine amidase AmpD -
  ACLRDL_RS12615 (ACLRDL_12615) nadC 2769017..2769907 (+) 891 WP_000665278.1 carboxylating nicotinate-nucleotide diphosphorylase -
  ACLRDL_RS12620 (ACLRDL_12620) - 2770152..2770619 (+) 468 WP_000995326.1 pilin -
  ACLRDL_RS12625 (ACLRDL_12625) pilB 2770620..2772308 (+) 1689 WP_000957148.1 type IV-A pilus assembly ATPase PilB Machinery gene
  ACLRDL_RS12630 (ACLRDL_12630) pilC 2772351..2773577 (+) 1227 WP_000648509.1 type II secretion system F family protein Machinery gene
  ACLRDL_RS12635 (ACLRDL_12635) pilD 2773635..2774510 (+) 876 WP_000418746.1 prepilin peptidase Machinery gene
  ACLRDL_RS12640 (ACLRDL_12640) coaE 2774507..2775115 (+) 609 WP_000011553.1 dephospho-CoA kinase -
  ACLRDL_RS12645 (ACLRDL_12645) zapD 2775147..2775887 (+) 741 WP_000207194.1 cell division protein ZapD -
  ACLRDL_RS12650 (ACLRDL_12650) yacG 2776045..2776242 (+) 198 WP_000162865.1 DNA gyrase inhibitor YacG -

Sequence

Protein


Download         Length: 408 a.a.        Molecular weight: 45094.37 Da        Isoelectric Point: 10.3744

>NTDB_id=1089402 ACLRDL_RS12630 WP_000648509.1 2772351..2773577(+) (pilC) [Vibrio cholerae isolate CTMA_1842]
MKATQTLPLKNYRWKGINSNGKKVSGQMLAISEIEVRDKLKDQHIQIKKLKKGSVSLLARLTHRVKSKDITILTRQLATM
LTTGVPIVQALKLVGDNHRKAEMKSILAQITKSVEAGTPLSKAMRTASAHFDTLYVDLVETGEMSGNLPEVFERLATYRE
KSEQLRAKVIKALIYPSMVVLVALGVSYLMLTMVIPEFESMFKGFGAELPWFTQQVLKLSHWVQAYSLWAFIAIAAAIFG
LKALRKNSFQIRLKTSRLGLKFPIIGNVLAKASIAKFSRTLATSFAAGIPILASLKTTAKTSGNVHFETAINEVHRDTAA
GMPMYIAMRNTDAFPEMVLQMVMIGEESGQLDDMLNKVATIYEFEVDNTVDNLGKILEPLIIVFLGTVVGGLVVAMYLPI
FNLMSVLG

Nucleotide


Download         Length: 1227 bp        

>NTDB_id=1089402 ACLRDL_RS12630 WP_000648509.1 2772351..2773577(+) (pilC) [Vibrio cholerae isolate CTMA_1842]
ATGAAAGCGACCCAAACCTTACCTCTGAAAAATTATCGCTGGAAAGGCATCAACAGCAACGGCAAAAAAGTTTCCGGCCA
GATGCTCGCCATCTCCGAAATCGAGGTGCGCGATAAGCTCAAAGATCAGCATATTCAGATCAAAAAACTCAAAAAAGGCA
GTGTCTCTTTATTGGCGCGCCTAACCCATCGCGTGAAAAGCAAAGACATCACGATTTTGACTCGGCAGTTGGCAACCATG
CTCACCACGGGCGTACCCATTGTGCAAGCCCTCAAGTTGGTGGGCGATAATCACCGTAAAGCTGAGATGAAATCGATTCT
GGCGCAAATCACCAAAAGCGTGGAAGCGGGCACGCCGCTTTCCAAGGCGATGCGCACCGCCAGCGCCCATTTTGATACCT
TGTATGTCGACTTAGTGGAAACCGGAGAGATGTCCGGTAACTTACCTGAAGTGTTTGAGCGTTTGGCCACCTACCGCGAG
AAAAGCGAGCAACTACGCGCCAAGGTGATTAAAGCGCTCATCTACCCCAGCATGGTGGTGTTGGTCGCGCTCGGAGTTTC
CTACTTAATGCTCACCATGGTCATCCCAGAATTTGAAAGCATGTTTAAAGGCTTTGGTGCCGAGCTGCCTTGGTTTACGC
AGCAAGTGCTGAAACTCTCACACTGGGTACAGGCTTACAGTTTATGGGCATTTATCGCCATCGCCGCGGCCATTTTTGGC
CTGAAAGCGCTGCGTAAAAACTCCTTCCAGATCCGTTTGAAAACCAGTCGCTTGGGGCTGAAATTTCCGATTATTGGCAA
TGTGCTCGCTAAGGCTTCCATCGCCAAATTCAGCCGCACCCTCGCCACCAGCTTTGCCGCGGGGATCCCGATCCTCGCCA
GTTTAAAAACCACTGCCAAAACCTCCGGCAATGTGCACTTTGAAACTGCGATTAATGAAGTACATCGTGATACTGCCGCC
GGTATGCCAATGTACATTGCCATGCGCAATACCGACGCTTTTCCCGAAATGGTGCTGCAAATGGTGATGATCGGTGAAGA
GTCTGGGCAATTAGACGACATGCTCAACAAGGTCGCGACCATCTATGAATTTGAAGTCGATAACACGGTCGATAACTTGG
GCAAGATTCTTGAACCACTGATCATCGTCTTTCTTGGGACGGTCGTGGGCGGCTTAGTGGTGGCGATGTACTTACCTATC
TTTAACTTAATGAGTGTATTGGGTTAG

Domains

Predicted by InterproScan.

Type II secretion system (T2SS), protein F (T2SSF)

(74-196)

Type II secretion system (T2SS), protein F (T2SSF)

(277-399)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  pilC Vibrio cholerae strain A1552

99.51

100

0.995
  pilC Vibrio campbellii strain DS40M4

73.5

98.039

0.721
  pilC Acinetobacter baumannii D1279779

42.647

100

0.426
  pilC Acinetobacter baylyi ADP1

42.015

99.755

0.419
  pilC Legionella pneumophila strain ERS1305867

42.211

97.549

0.412
  pilC Pseudomonas stutzeri DSM 10701

42.172

97.059

0.409
  pilG Neisseria gonorrhoeae MS11

41.045

98.529

0.404
  pilG Neisseria meningitidis 44/76-A

40.547

98.529

0.4

Multiple sequence alignment