Detailed information NTG1089297

Bioinformatically predicted

Overview

• Name: dprA
• Type: Machinery gene
• Locus tag: ACLSIV_RS06585
• Genome accession: NZ_CP178385
• Coordinates: 1343988..1344839 (-)
• Length: 283 a.a.
• NCBI ID: WP_061774798.1
• Uniprot ID: A0A2A5SB33
• Organism: Lactococcus raffinolactis strain GCULR
• Function: ssDNA binding; loading RecA onto ssDNA (predicted from homology) DNA processing

Genomic Context

Location: 1338988..1349839

Locus tag	Gene name	Coordinates (strand)	Size (bp)	Protein ID	Product	Description
ACLSIV_RS06565 (ACLSIV_06565)	purB	1339750..1341042 (-)	1293	WP_096039219.1	adenylosuccinate lyase	-
ACLSIV_RS06570 (ACLSIV_06570)	purK	1341063..1342121 (-)	1059	WP_096824057.1	5-(carboxyamino)imidazole ribonucleotide synthase	-
ACLSIV_RS06575 (ACLSIV_06575)	purE	1342114..1342608 (-)	495	WP_096039221.1	5-(carboxyamino)imidazole ribonucleotide mutase	-
ACLSIV_RS06580 (ACLSIV_06580)	purD	1342601..1343854 (-)	1254	WP_172506640.1	phosphoribosylamine--glycine ligase	-
ACLSIV_RS06585 (ACLSIV_06585)	dprA	1343988..1344839 (-)	852	WP_061774798.1	DNA-processing protein DprA	Machinery gene
ACLSIV_RS06590 (ACLSIV_06590)	-	1344921..1345877 (-)	957	WP_061774799.1	ABC transporter permease	-
ACLSIV_RS06595 (ACLSIV_06595)	-	1345877..1346953 (-)	1077	WP_061774800.1	ABC transporter permease	-
ACLSIV_RS06600 (ACLSIV_06600)	-	1346956..1348470 (-)	1515	WP_061774801.1	ABC transporter ATP-binding protein	-
ACLSIV_RS06605 (ACLSIV_06605)	deoD	1348619..1349329 (-)	711	WP_061774802.1	purine-nucleoside phosphorylase	-

Sequence

Protein

• Length: 283 a.a.
• Molecular weight: 32073.10 Da
• Isoelectric Point: 8.4451

>NTDB_id=1089297 ACLSIV_RS06585 WP_061774798.1 1343988..1344839(-) (dprA) [Lactococcus raffinolactis strain GCULR]
MQNFDIYRLKKAGMSNLMVYQFLTLVADSQERNPLVCLPAFIRQVKMKQSAHFWEKFRSFDIKAIREEFQKFPSFSIQDK
VYPEALREIYNPPVLLFYQGDLSLLHQTKLGFVGSRDCSQVGVASVQKIIKELQDRFVIVSGLARGIDTASHIAAMKNGG
KTIAVIGTGLDVNYPRENYQLQAYIAKHQLLLSEYGPDEKPLKYHFPERNRIIAGLSRGVLVTEAKLRSGSLITCERAME
EGRDVFAVPGNIIDGVSDGCHHLIQEGAKLIFHGRDILMEYDM

Nucleotide

• Length: 852 bp

>NTDB_id=1089297 ACLSIV_RS06585 WP_061774798.1 1343988..1344839(-) (dprA) [Lactococcus raffinolactis strain GCULR]
ATGCAAAATTTCGATATCTATCGCTTAAAAAAAGCTGGCATGTCAAACTTAATGGTTTATCAGTTTTTAACCCTAGTTGC
AGACAGTCAAGAAAGAAACCCCTTGGTCTGTTTACCAGCCTTTATTCGACAAGTTAAGATGAAACAGTCAGCTCATTTTT
GGGAAAAATTCCGATCATTTGACATAAAAGCCATACGAGAGGAGTTTCAGAAATTTCCGTCTTTCTCAATTCAGGATAAG
GTCTATCCTGAAGCTTTGCGAGAAATTTATAATCCGCCCGTCTTACTTTTTTATCAGGGAGATTTGAGCTTGCTTCATCA
GACCAAACTGGGTTTTGTTGGCAGTCGAGATTGCAGTCAAGTAGGGGTTGCCAGTGTTCAGAAGATCATCAAAGAATTGC
AAGATCGCTTTGTGATTGTTAGTGGCTTAGCACGGGGCATTGATACAGCTAGTCATATTGCTGCTATGAAAAATGGAGGT
AAGACAATTGCTGTGATTGGAACAGGGTTAGATGTGAATTATCCACGAGAAAATTATCAACTGCAGGCTTATATCGCAAA
ACATCAGCTCCTTTTATCAGAATATGGGCCAGATGAAAAACCTTTGAAGTATCATTTTCCAGAGCGTAATCGAATTATTG
CAGGTCTATCTCGTGGTGTATTAGTGACAGAAGCCAAGCTCCGCTCAGGTAGTTTGATTACCTGTGAGCGGGCTATGGAA
GAGGGGAGAGATGTGTTTGCCGTTCCAGGTAATATCATAGATGGGGTGTCAGATGGCTGCCACCATTTGATTCAGGAAGG
TGCAAAACTGATTTTCCATGGTCGAGATATATTAATGGAGTACGATATGTGA

Domains

Predicted by InterproScan.

• DNA recombination-mediator protein A (DNA_processg_A) (75-271)

• DNA processing protein A sterile alpha motif domain (SAM_DprA) (1-64)

3D structure

• AlphaFold DB: A0A2A5SB33

Similar proteins

Only experimentally validated proteins are listed.

Protein	Organism	Identities (%)	Coverage (%)	Ha-value
dprA	Streptococcus mutans UA159	62.898	100	0.629
dprA	Lactococcus lactis subsp. cremoris KW2	60.498	99.293	0.601
dprA/cilB/dalA	Streptococcus mitis NCTC 12261	58.156	99.647	0.58
dprA/cilB/dalA	Streptococcus pneumoniae Rx1	58.156	99.647	0.58
dprA/cilB/dalA	Streptococcus pneumoniae D39	58.156	99.647	0.58
dprA/cilB/dalA	Streptococcus pneumoniae R6	58.156	99.647	0.58
dprA/cilB/dalA	Streptococcus pneumoniae TIGR4	58.156	99.647	0.58
dprA/cilB/dalA	Streptococcus mitis SK321	57.801	99.647	0.576
dprA	Bacillus subtilis subsp. subtilis str. 168	49.321	78.092	0.385
dprA	Legionella pneumophila strain ERS1305867	40.23	92.226	0.371
dprA	Staphylococcus aureus MW2	35.664	100	0.36