Detailed information NTG1088544

Bioinformatically predicted

Overview

• Name: comEA
• Type: Machinery gene
• Locus tag: ACLFAM_RS03155
• Genome accession: NZ_CP178226
• Coordinates: 659801..660448 (-)
• Length: 215 a.a.
• NCBI ID: WP_010906131.1
• Uniprot ID: A0A3N6KQH1
• Organism: Lactococcus lactis strain L-WY410
• Function: dsDNA binding to the cell surface (predicted from homology) DNA binding and uptake

Genomic Context

Location: 654801..665448

Locus tag	Gene name	Coordinates (strand)	Size (bp)	Protein ID	Product	Description
ACLFAM_RS03130 (ACLFAM_03130)	atpF	654870..655376 (-)	507	WP_010906128.1	F0F1 ATP synthase subunit B	-
ACLFAM_RS03135 (ACLFAM_03135)	atpB	655391..656104 (-)	714	WP_004255255.1	F0F1 ATP synthase subunit A	-
ACLFAM_RS03140 (ACLFAM_03140)	-	656149..656364 (-)	216	WP_004255250.1	F0F1 ATP synthase subunit C	-
ACLFAM_RS03145 (ACLFAM_03145)	-	656549..657325 (-)	777	WP_039115826.1	alpha/beta hydrolase	-
ACLFAM_RS03150 (ACLFAM_03150)	comEC	657610..659820 (-)	2211	WP_039115828.1	DNA internalization-related competence protein ComEC/Rec2	Machinery gene
ACLFAM_RS03155 (ACLFAM_03155)	comEA	659801..660448 (-)	648	WP_010906131.1	ComEA family DNA-binding protein	Machinery gene
ACLFAM_RS03160 (ACLFAM_03160)	-	660508..661869 (-)	1362	WP_039115830.1	ABC transporter permease	-
ACLFAM_RS03165 (ACLFAM_03165)	-	661866..662798 (-)	933	WP_004255238.1	ABC transporter ATP-binding protein	-
ACLFAM_RS03170 (ACLFAM_03170)	-	662903..663247 (-)	345	WP_259747958.1	hypothetical protein	-
ACLFAM_RS03175 (ACLFAM_03175)	-	663405..663968 (-)	564	WP_004255233.1	GNAT family N-acetyltransferase	-
ACLFAM_RS03180 (ACLFAM_03180)	-	664141..665319 (-)	1179	WP_012898345.1	SLC13 family permease	-

Sequence

Protein

• Length: 215 a.a.
• Molecular weight: 23624.76 Da
• Isoelectric Point: 4.8659

>NTDB_id=1088544 ACLFAM_RS03155 WP_010906131.1 659801..660448(-) (comEA) [Lactococcus lactis strain L-WY410]
MDKILEKVKEYWKMIVLVVCGLIAGGIFYVLTNGQKPTTNLSVENLSSVSRQSSVSKFSEPNEKSVSKIMVDLKGAVTKP
NVYQISSDERLVDLIKEAGGFTDQADQKSINLSAKLKDEEVIYVPKIGENSSTENTDSSANSVTSQVSTTTEKININQAD
LTELQKLTGVGQKKAQDIIDFRTKNGDFKSLEDLGKVSGFGDKTLEKLKDELCFE

Nucleotide

• Length: 648 bp

>NTDB_id=1088544 ACLFAM_RS03155 WP_010906131.1 659801..660448(-) (comEA) [Lactococcus lactis strain L-WY410]
ATGGATAAGATTTTAGAAAAAGTAAAAGAATATTGGAAAATGATTGTTTTAGTTGTTTGTGGGCTCATTGCTGGTGGGAT
TTTTTACGTTTTAACCAACGGTCAAAAGCCAACTACAAATCTGTCAGTAGAAAATTTAAGTTCTGTCAGCAGGCAAAGCT
CTGTCAGTAAATTTAGTGAACCTAATGAAAAATCTGTCAGTAAAATTATGGTTGATTTAAAAGGTGCGGTAACAAAGCCT
AATGTCTATCAAATTTCGTCAGATGAACGTCTAGTTGATCTAATTAAGGAAGCTGGTGGTTTTACTGACCAAGCTGACCA
GAAATCAATTAATCTGTCAGCAAAATTAAAAGATGAAGAAGTAATTTATGTCCCAAAAATTGGTGAAAATTCAAGCACAG
AAAATACTGATAGCTCTGCTAATTCGGTCACTAGTCAAGTCTCAACAACCACTGAAAAAATAAATATCAATCAAGCAGAT
TTGACTGAACTACAAAAATTAACAGGTGTTGGTCAGAAAAAAGCTCAAGACATCATCGATTTTCGGACGAAAAATGGTGA
TTTCAAATCACTTGAAGACTTGGGAAAAGTTTCTGGTTTTGGAGATAAAACACTAGAAAAATTGAAAGATGAGTTGTGTT
TTGAATAA

Domains

Predicted by InterproScan.

• SLBB domain (SLBB) (70-125)

• Helix-hairpin-helix motif (HHH_3) (151-212)

3D structure

• AlphaFold DB: A0A3N6KQH1

Similar proteins

Only experimentally validated proteins are listed.

Protein	Organism	Identities (%)	Coverage (%)	Ha-value
comEA	Lactococcus lactis subsp. cremoris KW2	76.147	100	0.772
comEA/celA/cilE	Streptococcus mitis SK321	44.907	100	0.451
comEA/celA/cilE	Streptococcus pneumoniae TIGR4	43.578	100	0.442
comEA/celA/cilE	Streptococcus pneumoniae Rx1	41.553	100	0.423
comEA/celA/cilE	Streptococcus pneumoniae D39	41.553	100	0.423
comEA/celA/cilE	Streptococcus pneumoniae R6	41.553	100	0.423
comEA/celA/cilE	Streptococcus mitis NCTC 12261	41.743	100	0.423
comEA	Latilactobacillus sakei subsp. sakei 23K	36.564	100	0.386