Detailed information    

insolico Bioinformatically predicted

Overview

Name   pilQ   Type   Machinery gene
Locus tag   ACLH0I_RS03175 Genome accession   NZ_CP176770
Coordinates   740618..742705 (+) Length   695 a.a.
NCBI ID   WP_411386270.1    Uniprot ID   -
Organism   Pseudomonas sp. MPB03     
Function   assembly of type IV pilus (predicted from homology)   
DNA binding and uptake

Genomic Context

Location: 735618..747705
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  ACLH0I_RS03155 (ACLH0I_03155) comM 737827..738891 (+) 1065 WP_186706085.1 type IV pilus assembly protein PilM Machinery gene
  ACLH0I_RS03160 (ACLH0I_03160) - 738891..739457 (+) 567 WP_186706084.1 PilN domain-containing protein -
  ACLH0I_RS03165 (ACLH0I_03165) pilO 739454..740077 (+) 624 WP_053117658.1 type 4a pilus biogenesis protein PilO -
  ACLH0I_RS03170 (ACLH0I_03170) - 740083..740604 (+) 522 WP_186706137.1 pilus assembly protein PilP -
  ACLH0I_RS03175 (ACLH0I_03175) pilQ 740618..742705 (+) 2088 WP_411386270.1 type IV pilus secretin PilQ Machinery gene
  ACLH0I_RS03180 (ACLH0I_03180) aroK 742710..743228 (+) 519 WP_060739556.1 shikimate kinase AroK -
  ACLH0I_RS03185 (ACLH0I_03185) aroB 743377..744477 (+) 1101 WP_411386271.1 3-dehydroquinate synthase -
  ACLH0I_RS03190 (ACLH0I_03190) - 744488..746050 (+) 1563 WP_411386272.1 AAA family ATPase -

Sequence

Protein


Download         Length: 695 a.a.        Molecular weight: 74996.06 Da        Isoelectric Point: 5.4217

>NTDB_id=1082825 ACLH0I_RS03175 WP_411386270.1 740618..742705(+) (pilQ) [Pseudomonas sp. MPB03]
MNRFFSALGISLWTALLSPMVLGAGLKTLDVAALPGDRVELKLTFDGPPPTPRGYTTEQPARIALDLPGVTNQLASRSRD
LGGGNARSATVVEAGDRTRLIIGLTQLAPYDTRIEGNNLFVVVGKGAAPKSTARNSTAQAPRPAVASAAPARSSAPVGKA
IRGVDFQRGTQGEGNVVIDLSDPSIAPDIQERDGKIILNFARTQLPEPLRVRLDVKDFATPVQFVNASAGSDRATISIEP
SGTFDYSTYQTDNKLTVSVRPMTVDDLQKRNADRAAYSGEKLSLNFQDIEVRSVLQLIADFTNLNLVASDTVQGGITLRL
QNVPWDQALDLVLKTKGLDKRKVGNVLLVAPADEIAARERQELESQKQIADLAPLRRELLQVNYAKAADIAKLFQSVTSA
EAKADERGTITVDERTNNIIAYQTQDRLDELRRIVAQLDIPVRQVMIEARIVEANVDYDKSLGVRWGGSIQNKGNWNSSG
VTTGTSTTIGTPGSTGTNQPFVDLGASGNTSGIGIAFITDNVLLDLELTAMEKTGNGEIVSQPKVVTSDKETAKILKGTE
IPYQEASSSGATSVSFKEASLSLEVTPQITPDNRIIMEVKVTKDEPDYLNKVQDVPPIKKNEVNAKVLVNDGETIVIGGV
FSNTQSKVVDKVPFLGDVPYLGRLFRRDVVSEKKSELLVFLTPRIMNNQAIAVSR

Nucleotide


Download         Length: 2088 bp        

>NTDB_id=1082825 ACLH0I_RS03175 WP_411386270.1 740618..742705(+) (pilQ) [Pseudomonas sp. MPB03]
ATGAACAGGTTTTTTTCAGCCCTCGGTATTTCGCTATGGACAGCGCTGTTGTCGCCGATGGTTCTTGGGGCCGGCCTGAA
AACGCTGGATGTGGCGGCGTTGCCGGGGGACCGTGTCGAGTTGAAACTTACCTTCGACGGACCGCCGCCGACACCGCGGG
GCTACACCACCGAACAACCGGCCCGGATTGCGCTGGACCTGCCGGGTGTGACCAATCAACTGGCCAGTAGAAGCCGCGAC
CTGGGAGGGGGCAACGCGCGTAGCGCCACCGTGGTCGAGGCGGGGGATCGCACCCGGTTGATCATCGGCCTGACTCAACT
GGCGCCGTATGACACCCGGATCGAAGGCAATAACCTGTTCGTGGTGGTCGGCAAGGGCGCCGCCCCGAAGAGCACTGCCC
GCAACAGCACCGCCCAGGCTCCACGCCCGGCGGTGGCCAGCGCGGCGCCTGCCAGGAGCAGTGCGCCGGTGGGCAAGGCG
ATCCGTGGGGTGGATTTCCAGCGCGGGACCCAGGGCGAAGGCAACGTGGTGATCGATTTGTCCGATCCGTCCATCGCCCC
GGACATCCAGGAGCGCGACGGCAAGATCATTCTCAATTTCGCCAGGACCCAATTGCCCGAGCCGCTGCGGGTGCGCCTGG
ACGTCAAGGACTTCGCCACCCCGGTGCAATTCGTCAATGCCAGCGCCGGTAGCGACCGGGCGACCATCAGCATCGAGCCC
AGCGGCACCTTCGACTACTCGACCTACCAGACCGACAACAAGCTCACCGTCAGCGTCCGGCCCATGACCGTCGACGACCT
GCAAAAACGCAATGCCGACCGCGCCGCCTACAGCGGCGAAAAACTCTCGCTCAACTTCCAGGACATCGAGGTGCGTTCGG
TCCTGCAATTGATTGCCGACTTTACCAACCTGAACCTGGTGGCCAGCGACACGGTGCAGGGCGGTATCACCCTGCGCTTG
CAGAATGTGCCTTGGGACCAGGCGCTGGACTTGGTGCTCAAGACCAAGGGGTTGGACAAGCGCAAGGTCGGCAACGTGCT
GCTGGTGGCCCCGGCCGATGAAATCGCAGCGCGGGAACGCCAGGAGCTGGAGTCGCAAAAGCAGATTGCCGACCTGGCGC
CACTGCGGCGTGAGCTGCTGCAGGTCAACTATGCGAAGGCTGCGGACATCGCCAAGCTGTTCCAATCGGTAACCAGCGCC
GAGGCGAAAGCCGACGAGCGCGGCACGATCACGGTGGATGAGCGAACCAACAACATCATTGCCTACCAGACCCAGGACCG
GCTCGACGAACTGCGCCGGATCGTGGCGCAGTTGGATATTCCGGTACGCCAGGTGATGATCGAGGCGCGAATTGTCGAGG
CCAACGTCGACTACGACAAAAGCCTGGGCGTGCGCTGGGGCGGTTCGATCCAGAACAAGGGCAACTGGAACTCCTCGGGG
GTGACCACCGGCACCTCCACCACCATCGGCACGCCGGGCAGTACCGGCACCAATCAGCCGTTCGTGGACCTGGGTGCCTC
GGGCAATACCTCGGGGATCGGCATTGCGTTCATCACCGACAACGTATTGCTGGACCTTGAACTGACGGCCATGGAGAAAA
CCGGTAACGGGGAAATCGTCTCCCAGCCCAAGGTGGTGACATCCGACAAGGAGACGGCCAAGATCCTCAAGGGCACGGAG
ATTCCTTATCAGGAAGCCAGCTCCAGCGGCGCCACCTCGGTGTCGTTCAAGGAGGCCTCGCTGTCCCTGGAGGTGACCCC
GCAAATCACCCCGGACAACCGCATCATCATGGAGGTCAAGGTCACCAAGGATGAGCCGGACTACCTGAACAAGGTGCAGG
ATGTGCCGCCGATCAAGAAAAACGAAGTCAACGCCAAGGTCTTGGTCAATGATGGCGAGACCATTGTGATTGGCGGGGTT
TTCTCAAATACTCAGAGCAAGGTCGTAGATAAGGTGCCATTTCTTGGCGATGTGCCGTATCTTGGCCGCCTTTTCCGGCG
TGATGTGGTTTCGGAGAAAAAATCCGAGCTGCTGGTGTTCCTCACTCCGCGTATCATGAATAACCAGGCGATTGCTGTGA
GTCGTTGA

Domains

Predicted by InterproScan.

Bacterial type II/III secretion system short domain (Secretin_N)

(379-444)

AMIN domain (AMIN)

(38-121)

Bacterial type II and III secretion system protein (Secretin)

(530-686)

Secretin and TonB N terminus short domain (STN)

(305-351)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  pilQ Pseudomonas aeruginosa PAK

72.954

100

0.757
  comQ Acinetobacter baylyi ADP1

41.81

100

0.419
  pilQ Acinetobacter baumannii strain A118

39.636

100

0.407
  pilQ Acinetobacter baumannii D1279779

39.636

100

0.407
  pilQ Legionella pneumophila strain Lp02

38.586

97.698

0.377
  pilQ Legionella pneumophila strain ERS1305867

38.462

97.266

0.374

Multiple sequence alignment