Detailed information    

insolico Bioinformatically predicted

Overview

Name   pilB   Type   Machinery gene
Locus tag   ACJRPX_RS01190 Genome accession   NZ_CP174475
Coordinates   266508..268211 (+) Length   567 a.a.
NCBI ID   WP_078306349.1    Uniprot ID   A0A1T0CJJ2
Organism   Moraxella lincolnii strain CCUG 52988     
Function   power the assembly of type IV pilus (predicted from homology)   
DNA binding and uptake

Genomic Context

Location: 261508..273211
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  ACJRPX_RS01160 (ACJRPX_01160) nusA 262076..263560 (-) 1485 WP_078306353.1 transcription termination factor NusA -
  ACJRPX_RS01165 (ACJRPX_01165) rimP 263621..264115 (-) 495 WP_078306352.1 ribosome maturation factor RimP -
  ACJRPX_RS01180 (ACJRPX_01180) secG 264753..265064 (-) 312 WP_078306351.1 preprotein translocase subunit SecG -
  ACJRPX_RS01185 (ACJRPX_01185) tpiA 265176..265997 (-) 822 WP_078306350.1 triose-phosphate isomerase -
  ACJRPX_RS01190 (ACJRPX_01190) pilB 266508..268211 (+) 1704 WP_078306349.1 type IV-A pilus assembly ATPase PilB Machinery gene
  ACJRPX_RS01195 (ACJRPX_01195) pilC 268389..269612 (+) 1224 WP_078306348.1 type II secretion system F family protein Machinery gene
  ACJRPX_RS01200 (ACJRPX_01200) pilD 269624..270529 (+) 906 WP_205760089.1 prepilin peptidase Machinery gene
  ACJRPX_RS01205 (ACJRPX_01205) coaE 270630..271247 (+) 618 WP_078306439.1 dephospho-CoA kinase -
  ACJRPX_RS01210 (ACJRPX_01210) rlmB 271259..272092 (-) 834 WP_078306347.1 23S rRNA (guanosine(2251)-2'-O)-methyltransferase RlmB -
  ACJRPX_RS01215 (ACJRPX_01215) - 272153..272488 (-) 336 WP_078306346.1 pyrimidine/purine nucleoside phosphorylase -

Sequence

Protein


Download         Length: 567 a.a.        Molecular weight: 62412.73 Da        Isoelectric Point: 5.0743

>NTDB_id=1077253 ACJRPX_RS01190 WP_078306349.1 266508..268211(+) (pilB) [Moraxella lincolnii strain CCUG 52988]
MSSSNMSFSGIAQRLVKEGLIAPDKMKAALSAVQQEKTSLIAYVVDNKLATQEKVASIVADEFGDPLLDIAELSDDSVPK
DLVDEKIVKKYSALPVFRRGQRLYVAMSDPSRLDAIDAISFNSRLSVQTVVVEYSKLVKKIESIFADSMTNLQDFSDGDF
DVDVDSDGDDETNVEGGAEDAPVVKFINKMLIDAIRMGASDLHFEPYEKSYRVRFRIDGVMQKVASPPVQIAGKIAARLK
VMSQMDISERRVPQDGRIKLKISKDKAIDFRVNSLPTLFGEKIVLRILDPSSAMLGIDMLGYEPDQKELFLEALHKPQGM
MLITGPTGSGKTVSLYTGLNILNTVESNISTAEDPVEINLEGINQVNVNPKVGLTFASALKSFLRQDPDIVMVGEIRDLE
TAEIAIKAAQTGHMVLSTLHTNSAPETLTRLRNMGVASFNIATSVNLVVAQRLARRLCKNCKRPASVPKQSLLEMGFTEE
DLSNPDNIIYEPVGCDQCREGYKGRVGIYEVMKVSNDISRIIMEDGNAIAIKDAALKNGFRDLRRSGIMKVLQGVTSIQE
MYRVTSE

Nucleotide


Download         Length: 1704 bp        

>NTDB_id=1077253 ACJRPX_RS01190 WP_078306349.1 266508..268211(+) (pilB) [Moraxella lincolnii strain CCUG 52988]
ATGTCATCATCGAATATGTCCTTTAGTGGCATTGCTCAAAGACTGGTCAAAGAAGGGCTGATTGCACCCGATAAGATGAA
AGCGGCATTGAGTGCTGTCCAACAAGAGAAAACCAGCTTAATTGCTTATGTGGTTGACAATAAGCTGGCAACACAAGAGA
AAGTTGCCAGCATCGTGGCAGATGAGTTTGGTGATCCTCTTCTTGACATTGCTGAACTGAGCGATGACAGCGTTCCTAAA
GATTTGGTTGATGAAAAGATTGTTAAGAAATACAGTGCGTTGCCTGTTTTTCGTCGTGGGCAACGTTTGTATGTGGCGAT
GAGTGACCCATCACGCCTTGATGCCATTGATGCCATCTCATTTAATTCTCGCTTGTCAGTGCAGACGGTGGTGGTGGAGT
ACAGTAAGCTGGTTAAGAAAATCGAAAGCATTTTTGCAGATTCGATGACCAATCTTCAAGACTTTAGTGATGGTGATTTT
GATGTTGATGTTGACAGCGATGGTGATGATGAAACCAATGTAGAGGGGGGGGCAGAAGATGCTCCTGTAGTGAAGTTTAT
TAATAAAATGCTCATAGATGCCATTCGCATGGGGGCATCGGATTTGCATTTTGAGCCTTATGAAAAATCCTATCGGGTGC
GTTTTCGCATTGATGGGGTGATGCAAAAAGTGGCTAGCCCTCCTGTCCAGATTGCTGGTAAAATTGCCGCACGCTTAAAG
GTGATGTCGCAGATGGACATTTCAGAACGGCGTGTGCCACAAGATGGGCGTATCAAACTTAAAATATCCAAAGATAAAGC
CATTGACTTTCGTGTTAACTCCTTACCGACTCTGTTTGGTGAAAAAATCGTTTTACGTATTTTAGATCCGTCTTCTGCCA
TGCTCGGAATTGACATGCTTGGTTATGAGCCTGACCAAAAAGAGCTGTTTTTAGAAGCCTTACACAAACCTCAAGGCATG
ATGCTCATCACAGGTCCAACAGGTTCGGGTAAAACGGTGTCGTTATACACAGGTCTTAATATCCTAAACACGGTAGAAAG
TAACATATCCACCGCCGAAGATCCTGTGGAGATTAACCTTGAGGGCATCAATCAGGTCAATGTTAACCCAAAAGTGGGTT
TGACCTTTGCCAGTGCGTTAAAGTCATTTTTACGTCAAGACCCTGACATTGTGATGGTCGGTGAGATTCGTGACCTTGAA
ACGGCTGAGATTGCCATCAAAGCGGCTCAAACGGGCCACATGGTGCTGTCTACTTTACACACCAACTCAGCACCTGAAAC
GTTGACTCGTTTGCGTAATATGGGCGTGGCATCATTTAACATCGCCACATCAGTCAACTTGGTGGTGGCTCAGCGCCTTG
CCAGAAGACTCTGTAAGAATTGTAAGCGACCTGCCAGTGTGCCTAAGCAAAGTTTGTTAGAAATGGGCTTTACTGAAGAA
GATTTGAGCAATCCTGATAATATCATCTATGAACCTGTTGGCTGTGATCAATGCCGTGAAGGTTATAAAGGGCGTGTTGG
TATTTATGAAGTCATGAAAGTCAGTAATGACATCTCTCGCATCATCATGGAAGATGGTAACGCCATTGCCATTAAGGACG
CAGCCCTTAAAAATGGTTTTCGAGATTTGCGCCGATCAGGCATCATGAAGGTCTTGCAAGGTGTCACCAGCATTCAAGAG
ATGTATCGAGTCACTTCTGAATAA

Domains

Predicted by InterproScan.

Type II secretion system (T2SS), protein E, N-terminal domain (MshEN)

(45-148)

Type II/IV secretion system protein (T2SSE)

(189-456)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure
  AlphaFold DB A0A1T0CJJ2

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  pilB Acinetobacter baumannii D1279779

68.972

99.471

0.686
  pilB Acinetobacter baylyi ADP1

66.488

98.942

0.658
  pilB Legionella pneumophila strain ERS1305867

52.504

100

0.536
  pilF Neisseria gonorrhoeae MS11

50.631

97.884

0.496
  pilB Vibrio parahaemolyticus RIMD 2210633

48.043

99.118

0.476
  pilB Vibrio cholerae strain A1552

48.37

97.354

0.471
  pilB Vibrio campbellii strain DS40M4

46.536

99.295

0.462
  pilF Thermus thermophilus HB27

36.525

99.471

0.363
  pilB Deinococcus radiodurans R1 = ATCC 13939 = DSM 20539

36.72

98.942

0.363

Multiple sequence alignment