Detailed information    

insolico Bioinformatically predicted

Overview

Name   pilU   Type   Machinery gene
Locus tag   ACJMQR_RS21130 Genome accession   NZ_CP174465
Coordinates   4398519..4399664 (-) Length   381 a.a.
NCBI ID   WP_136663427.1    Uniprot ID   A0A4T1ZZP4
Organism   Pseudomonas leptonychotis strain TMP19     
Function   assembly of type IV pilus (predicted from homology)   
DNA binding and uptake

Genomic Context

Location: 4393519..4404664
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  ACJMQR_RS21105 (ACJMQR_21105) - 4393571..4394581 (+) 1011 WP_405119267.1 aspartate carbamoyltransferase catalytic subunit -
  ACJMQR_RS21110 (ACJMQR_21110) - 4394581..4395852 (+) 1272 WP_405119268.1 dihydroorotase -
  ACJMQR_RS21115 (ACJMQR_21115) - 4395907..4396305 (-) 399 WP_136663424.1 TM2 domain-containing protein -
  ACJMQR_RS21120 (ACJMQR_21120) - 4396464..4397675 (+) 1212 WP_405119269.1 putative DNA modification/repair radical SAM protein -
  ACJMQR_RS21125 (ACJMQR_21125) - 4397675..4398553 (+) 879 WP_405119270.1 TIGR03915 family putative DNA repair protein -
  ACJMQR_RS21130 (ACJMQR_21130) pilU 4398519..4399664 (-) 1146 WP_136663427.1 PilT/PilU family type 4a pilus ATPase Machinery gene
  ACJMQR_RS21135 (ACJMQR_21135) pilT 4399771..4400805 (-) 1035 WP_136663428.1 type IV pilus twitching motility protein PilT Machinery gene
  ACJMQR_RS21140 (ACJMQR_21140) - 4400869..4401555 (+) 687 WP_405119271.1 YggS family pyridoxal phosphate-dependent enzyme -
  ACJMQR_RS21145 (ACJMQR_21145) proC 4401584..4402402 (+) 819 WP_136663430.1 pyrroline-5-carboxylate reductase -
  ACJMQR_RS21150 (ACJMQR_21150) - 4402412..4403002 (+) 591 WP_136663431.1 YggT family protein -

Sequence

Protein


Download         Length: 381 a.a.        Molecular weight: 42562.76 Da        Isoelectric Point: 6.6648

>NTDB_id=1077181 ACJMQR_RS21130 WP_136663427.1 4398519..4399664(-) (pilU) [Pseudomonas leptonychotis strain TMP19]
MEFEKLLRLMVEKGGSDLFITAGVPPSMKVNGKIMPITKTPMSPEQTRETVHSVMNEQQRRDFTENHECNFAISARGVGR
FRVSAFYQRNLAGMVLRRIETNIPTLDELKLPEILKKLALTKRGLVLFVGATGTGKSTSLAAMIGYRNKNSSGHIISIED
PIEYIHQHQSCIVTQREVGIDTESFEVALKNTLRQAPDVILIGEVRTRETMDHAVAFAETGHLCLATLHANNANQALDRI
INFFPADRQNQVWMDLSLNLKAIVAQQLIPTPDGKGRRAVIEVLINTPLASDLIRKGEVHELKALMKRSTEQGMQTFDQA
LYNLYSQGEITYEDALLYADSANDLRLMIKLGSESDGDHLSSMAQGLSLEVSEEDPGRRFR

Nucleotide


Download         Length: 1146 bp        

>NTDB_id=1077181 ACJMQR_RS21130 WP_136663427.1 4398519..4399664(-) (pilU) [Pseudomonas leptonychotis strain TMP19]
ATGGAATTCGAAAAACTGCTGCGCCTGATGGTGGAAAAGGGCGGCTCTGACCTGTTTATCACTGCCGGTGTACCGCCGTC
GATGAAGGTCAATGGCAAGATCATGCCAATCACCAAGACGCCCATGTCGCCTGAGCAGACGCGTGAAACTGTGCACTCGG
TGATGAATGAGCAGCAGCGCCGCGATTTCACGGAGAACCATGAGTGCAATTTCGCGATCAGCGCCCGCGGTGTGGGCCGT
TTCCGCGTCAGTGCCTTTTACCAGCGCAACTTGGCCGGTATGGTGCTGCGCCGCATCGAGACCAATATCCCGACGTTGGA
CGAGCTGAAACTGCCGGAAATTCTCAAGAAACTGGCGCTGACCAAGCGCGGTCTGGTGTTATTCGTGGGAGCCACCGGCA
CGGGTAAGTCGACCTCGCTGGCAGCAATGATTGGCTACCGCAACAAGAACAGCAGCGGTCATATCATTTCCATCGAAGAC
CCGATCGAATACATCCACCAGCACCAGAGCTGCATCGTCACTCAGCGTGAGGTGGGCATCGACACCGAGTCGTTCGAAGT
AGCGCTGAAGAACACCCTGCGCCAGGCGCCGGACGTGATTCTGATTGGTGAGGTGCGCACCCGCGAGACCATGGACCACG
CCGTGGCGTTCGCTGAAACCGGTCACTTATGCCTGGCCACGCTGCACGCCAACAACGCCAACCAGGCGCTGGACCGCATT
ATCAACTTCTTCCCGGCGGACCGGCAGAACCAGGTGTGGATGGATCTGTCGCTCAACCTCAAAGCCATCGTTGCTCAGCA
ACTGATCCCAACGCCGGATGGTAAGGGCCGCCGTGCGGTGATCGAGGTGTTGATCAATACGCCACTGGCTTCCGATCTGA
TCCGTAAGGGTGAGGTGCATGAGCTCAAGGCGTTGATGAAACGCTCCACCGAGCAGGGTATGCAGACCTTCGATCAGGCG
CTGTACAACCTATACAGCCAAGGCGAGATTACCTATGAAGACGCGTTGCTTTACGCTGACTCAGCCAACGACCTGCGTCT
GATGATCAAGCTGGGTTCTGAAAGTGACGGTGATCACTTGAGCAGCATGGCTCAGGGGCTGTCGCTGGAAGTCAGTGAGG
AAGATCCGGGTCGGCGCTTTCGCTGA

Domains

Predicted by InterproScan.

Type II/IV secretion system protein (T2SSE)

(111-270)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure
  AlphaFold DB A0A4T1ZZP4

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  pilU Pseudomonas stutzeri DSM 10701

91.339

100

0.913
  pilU Acinetobacter baylyi ADP1

64.706

93.701

0.606
  pilU Vibrio cholerae strain A1552

58.857

91.864

0.541
  pilT Deinococcus radiodurans R1 = ATCC 13939 = DSM 20539

43.75

88.189

0.386
  pilT Neisseria gonorrhoeae MS11

41.791

87.927

0.367
  pilT Neisseria meningitidis 8013

41.791

87.927

0.367
  pilT Pseudomonas aeruginosa PAK

41.493

87.927

0.365

Multiple sequence alignment