Detailed information    

insolico Bioinformatically predicted

Overview

Name   pilB   Type   Machinery gene
Locus tag   ACJGJ0_RS08215 Genome accession   NZ_CP174366
Coordinates   1919500..1921236 (-) Length   578 a.a.
NCBI ID   WP_033482835.1    Uniprot ID   -
Organism   Xanthomonas citri pv. mangiferaeindicae strain GXBS06     
Function   power the assembly of type IV pilus (predicted from homology)   
DNA binding and uptake

Genomic Context

Location: 1914500..1926236
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  ACJGJ0_RS08195 (ACJGJ0_08195) - 1915463..1917076 (+) 1614 WP_003488599.1 PAS domain-containing sensor histidine kinase -
  ACJGJ0_RS08200 (ACJGJ0_08200) pilR 1917405..1918799 (+) 1395 WP_003488597.1 sigma-54 dependent transcriptional regulator Regulator
  ACJGJ0_RS08205 (ACJGJ0_08205) - 1918997..1919152 (-) 156 WP_003488595.1 hypothetical protein -
  ACJGJ0_RS08210 (ACJGJ0_08210) pilB 1919284..1919403 (-) 120 WP_033482837.1 hypothetical protein Machinery gene
  ACJGJ0_RS08215 (ACJGJ0_08215) pilB 1919500..1921236 (-) 1737 WP_033482835.1 type IV-A pilus assembly ATPase PilB Machinery gene
  ACJGJ0_RS08220 (ACJGJ0_08220) pilA2 1921278..1921691 (-) 414 WP_005921365.1 pilin Machinery gene
  ACJGJ0_RS08225 (ACJGJ0_08225) comP 1921788..1922216 (-) 429 WP_005921364.1 pilin Machinery gene
  ACJGJ0_RS08230 (ACJGJ0_08230) pilC 1922561..1923820 (+) 1260 WP_033483308.1 type II secretion system F family protein Machinery gene
  ACJGJ0_RS08235 (ACJGJ0_08235) - 1923827..1924690 (+) 864 WP_003491180.1 A24 family peptidase -
  ACJGJ0_RS08240 (ACJGJ0_08240) coaE 1924704..1925312 (+) 609 WP_003491178.1 dephospho-CoA kinase -

Sequence

Protein


Download         Length: 578 a.a.        Molecular weight: 62654.75 Da        Isoelectric Point: 5.7586

>NTDB_id=1076885 ACJGJ0_RS08215 WP_033482835.1 1919500..1921236(-) (pilB) [Xanthomonas citri pv. mangiferaeindicae strain GXBS06]
MNVAIGSNLVGITGIARRLVQDGALDEASARAAMDHATQAKVPLPQWFAEKKLVTAAQLAAANAVEFGMPLMDVSAFDAN
QNAVKLVSEELLQKHQVLPLFKRGNRLFVGVSNPTQTRALDDIKFHTNLVVEAILVDEDQIRRTLEQWQASNASLGSSLG
NDDEEMGDLDVSAGDEDMGAGGDSGVDAKGDDTPVVKFVNKVLVDAIRRGASDIHFEPYEDDYRVRLRIDGLLKNVAKAP
VKLNQRIAARLKVMSQLDIAEKRVPQDGRIKLNLSKTKQIDFRVSTLPTLFGEKVVLRILDGSAAKLGIDKLGYEPDQQK
LFLEAIHKPYGMVLVTGPTGSGKTVSLYTALGILNDETRNISTAEDPVEIRLPGVNQVQQNNKRGMTFAAALRSFLRQDP
DIIMVGEIRDLETAEIAIKAAQTGHMVLSTLHTNDAPQTIARLMNMGIAPYNITSSVTLVIAQRLARRLCNNCKRKSTLP
DNALLAEGFTPAQLAAGIELYEAVGCDECTEGYKGRTGIYQVMPMTDEIGAIVLEGGNAMQIAEAAQAIGIRDLRQSALM
KAAHGVTSLAEINRVTKD

Nucleotide


Download         Length: 1737 bp        

>NTDB_id=1076885 ACJGJ0_RS08215 WP_033482835.1 1919500..1921236(-) (pilB) [Xanthomonas citri pv. mangiferaeindicae strain GXBS06]
ATGAACGTTGCAATCGGCTCAAATCTAGTTGGAATTACTGGTATTGCGCGGCGCCTTGTTCAGGATGGTGCGCTTGATGA
GGCGAGCGCGCGTGCGGCAATGGATCACGCAACCCAGGCCAAAGTTCCTTTACCGCAATGGTTCGCCGAAAAGAAGCTTG
TAACAGCCGCACAACTCGCGGCCGCCAACGCAGTCGAGTTTGGCATGCCTTTGATGGATGTATCGGCGTTCGATGCCAAC
CAGAACGCGGTCAAGCTGGTCAGTGAAGAGTTGCTCCAGAAGCACCAGGTGCTGCCGCTGTTCAAGCGCGGCAACCGGTT
GTTCGTGGGGGTGAGCAATCCGACCCAGACCCGGGCGCTGGACGACATCAAGTTCCATACGAACTTGGTGGTCGAGGCCA
TTCTTGTCGACGAGGATCAGATCCGCCGGACGCTCGAACAGTGGCAGGCCAGCAACGCGTCGTTGGGTTCGTCGCTTGGC
AACGACGACGAGGAAATGGGGGATCTGGACGTCTCGGCCGGCGACGAGGACATGGGCGCCGGCGGGGATTCCGGGGTCGA
TGCCAAGGGCGACGACACGCCGGTGGTGAAGTTCGTCAACAAGGTGCTGGTGGATGCGATCAGGCGGGGAGCCTCGGACA
TCCATTTCGAGCCGTATGAAGACGACTACCGGGTGCGCTTGCGCATCGATGGCCTGTTGAAGAACGTGGCCAAGGCGCCG
GTGAAGCTGAACCAGCGCATCGCAGCGCGGTTGAAGGTGATGTCGCAGCTGGATATCGCCGAGAAGCGGGTGCCGCAGGA
CGGGCGCATCAAGCTCAACCTGTCCAAGACCAAGCAGATCGACTTCCGTGTCAGCACCTTGCCGACCCTGTTCGGAGAGA
AGGTGGTGCTGCGTATCCTGGACGGCAGCGCGGCCAAGCTGGGCATCGACAAGCTGGGCTACGAGCCGGACCAGCAGAAG
CTGTTCCTGGAAGCGATCCACAAGCCCTATGGCATGGTGCTGGTGACCGGGCCGACCGGCTCGGGCAAGACGGTGTCGTT
GTACACCGCGCTGGGAATTCTCAACGACGAGACGCGCAATATCTCCACCGCGGAGGATCCGGTCGAAATCCGCTTGCCTG
GCGTCAATCAGGTGCAGCAGAACAACAAGCGTGGCATGACCTTCGCCGCGGCTTTGCGCTCGTTCCTGCGCCAGGACCCG
GACATCATCATGGTCGGCGAAATCCGTGACCTGGAGACTGCCGAGATTGCGATCAAGGCGGCGCAGACGGGTCACATGGT
GCTGTCGACGTTGCACACCAACGATGCGCCGCAGACCATCGCGCGTCTGATGAACATGGGCATCGCGCCCTACAACATCA
CCTCGTCGGTGACGCTGGTGATCGCGCAGCGTCTGGCGCGGCGCTTGTGCAACAACTGCAAGCGCAAGTCGACGCTGCCT
GACAACGCATTGCTGGCCGAAGGGTTCACGCCTGCACAGCTTGCCGCCGGGATCGAGCTGTATGAGGCGGTCGGTTGCGA
TGAGTGCACCGAAGGTTACAAGGGGCGTACCGGTATCTACCAGGTGATGCCGATGACCGACGAGATCGGCGCAATCGTGC
TGGAAGGCGGCAATGCGATGCAGATCGCCGAGGCCGCGCAGGCGATCGGTATCCGCGATTTGCGGCAGTCGGCGTTGATG
AAGGCTGCGCACGGGGTGACCAGCCTGGCCGAGATCAATCGTGTGACGAAGGACTAA

Domains

Predicted by InterproScan.

Type II secretion system (T2SS), protein E, N-terminal domain (MshEN)

(49-153)

Type II/IV secretion system protein (T2SSE)

(201-468)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  pilB Acinetobacter baumannii D1279779

56.162

98.27

0.552
  pilB Acinetobacter baylyi ADP1

55.282

98.27

0.543
  pilB Legionella pneumophila strain ERS1305867

51.73

100

0.517
  pilB Vibrio cholerae strain A1552

48.962

100

0.49
  pilF Neisseria gonorrhoeae MS11

49.296

98.27

0.484
  pilB Vibrio parahaemolyticus RIMD 2210633

46.429

96.886

0.45
  pilB Vibrio campbellii strain DS40M4

45.167

98.443

0.445

Multiple sequence alignment