Detailed information    

insolico Bioinformatically predicted

Overview

Name   pilC   Type   Machinery gene
Locus tag   ACJEHH_RS01945 Genome accession   NZ_CP174238
Coordinates   448034..449254 (+) Length   406 a.a.
NCBI ID   WP_011215549.1    Uniprot ID   Q5WWF0
Organism   Legionella pneumophila strain SPF587     
Function   assembly of type IV pilus (predicted from homology)   
DNA binding and uptake

Genomic Context

Location: 443034..454254
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  ACJEHH_RS01925 (ACJEHH_01925) - 444181..444750 (-) 570 WP_011215552.1 hypoxanthine-guanine phosphoribosyltransferase -
  ACJEHH_RS01930 (ACJEHH_01930) - 444793..445119 (-) 327 WP_011213802.1 DUF1820 family protein -
  ACJEHH_RS01935 (ACJEHH_01935) - 445302..446045 (-) 744 WP_011215551.1 methyltransferase domain-containing protein -
  ACJEHH_RS01940 (ACJEHH_01940) pilB 446304..448028 (+) 1725 WP_011215550.1 type IV-A pilus assembly ATPase PilB Machinery gene
  ACJEHH_RS01945 (ACJEHH_01945) pilC 448034..449254 (+) 1221 WP_011215549.1 type II secretion system F family protein Machinery gene
  ACJEHH_RS01950 (ACJEHH_01950) pilD 449300..450163 (+) 864 WP_011215548.1 A24 family peptidase Machinery gene
  ACJEHH_RS01955 (ACJEHH_01955) - 450177..451382 (-) 1206 WP_011215547.1 cyclic nucleotide-binding domain-containing protein -
  ACJEHH_RS01960 (ACJEHH_01960) - 451490..452083 (-) 594 WP_011213808.1 hypothetical protein -
  ACJEHH_RS01965 (ACJEHH_01965) - 452099..452554 (-) 456 WP_011215546.1 hypothetical protein -
  ACJEHH_RS01970 (ACJEHH_01970) - 452688..453503 (+) 816 WP_011215545.1 pyruvate, water dikinase regulatory protein -

Sequence

Protein


Download         Length: 406 a.a.        Molecular weight: 44693.84 Da        Isoelectric Point: 9.6052

>NTDB_id=1076208 ACJEHH_RS01945 WP_011215549.1 448034..449254(+) (pilC) [Legionella pneumophila strain SPF587]
MDKNSPPLLTFHYQGINKAGQKMEGDIQARSLAIAKADLRKQGIVTNKVVKKRKPLFDRKNKKITQADITVFSRQLATMI
ESGIPLVQAFDIVAKGQSNKRLKDLIETIKHDIETGLTLAESLIKHPLYFNELFCNLVDAGEKSGSLDIMLDKVATYKEK
IEIIKKKIKKALTYPIAVMVVALLVTAGLLIYVVPQFESLFKGFGADLPAMTRAVITMSEFMQAYWYIIFGALGGVVYSF
FYAKNHSLEFAQTIDRVMLKFPVIGPILEKAAIARFARTLSITFAAGLPLVEALKSVAGATGNIIYAKATDKIREEVATG
QQMFTAMENTHLFPNMVIQMVAIGEESGALEKMLSKVADFYEEEVNNAVDALSSLLEPIIMSILGILVGGLVVAMYLPIF
KLGSAV

Nucleotide


Download         Length: 1221 bp        

>NTDB_id=1076208 ACJEHH_RS01945 WP_011215549.1 448034..449254(+) (pilC) [Legionella pneumophila strain SPF587]
ATGGATAAAAATAGCCCACCCCTATTAACCTTTCATTATCAAGGCATTAATAAAGCCGGTCAAAAAATGGAGGGTGATAT
TCAAGCCAGAAGCTTGGCAATAGCTAAAGCTGATTTGCGTAAGCAGGGGATTGTTACCAATAAAGTAGTAAAAAAAAGAA
AACCTTTGTTTGATAGAAAAAATAAAAAAATCACACAAGCAGATATTACTGTATTTAGTCGCCAATTAGCGACGATGATC
GAGTCAGGAATACCATTAGTGCAAGCATTTGATATTGTTGCCAAAGGACAAAGCAATAAAAGACTTAAAGACTTAATAGA
AACAATTAAGCACGATATCGAAACGGGGCTAACGCTGGCAGAATCCTTAATTAAACATCCACTCTATTTTAATGAATTAT
TTTGTAATCTTGTCGATGCAGGTGAAAAATCAGGCTCATTAGATATCATGCTTGACAAGGTTGCAACGTATAAAGAAAAA
ATTGAAATCATAAAAAAGAAAATAAAAAAAGCTCTCACTTACCCAATTGCTGTTATGGTTGTCGCACTTTTGGTTACTGC
GGGATTATTAATCTATGTGGTGCCTCAATTTGAATCATTGTTTAAAGGATTTGGAGCTGATTTGCCAGCGATGACTAGAG
CTGTGATCACTATGTCTGAATTTATGCAGGCTTATTGGTATATTATTTTTGGAGCTTTGGGCGGGGTTGTCTATAGCTTT
TTTTATGCTAAAAATCATTCTTTGGAGTTTGCGCAAACCATTGACAGAGTGATGCTAAAATTCCCTGTAATTGGTCCTAT
TTTAGAGAAAGCAGCAATTGCTAGATTCGCGAGGACATTATCCATTACTTTTGCTGCTGGATTACCCCTGGTTGAAGCAT
TGAAATCTGTAGCTGGAGCGACCGGGAATATTATTTATGCCAAGGCGACTGATAAAATCAGAGAAGAAGTTGCAACAGGA
CAGCAAATGTTTACTGCTATGGAAAATACTCATTTATTTCCAAATATGGTAATTCAAATGGTAGCAATTGGTGAGGAATC
AGGTGCCCTTGAAAAAATGTTAAGCAAAGTAGCTGATTTTTATGAGGAAGAAGTAAATAATGCTGTAGATGCCCTGAGTA
GTTTATTGGAGCCGATAATTATGTCAATATTAGGTATACTGGTTGGGGGATTGGTGGTGGCTATGTATTTACCTATTTTC
AAACTGGGTTCTGCTGTTTAA

Domains

Predicted by InterproScan.

Type II secretion system (T2SS), protein F (T2SSF)

(276-398)

Type II secretion system (T2SS), protein F (T2SSF)

(72-195)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure
  AlphaFold DB Q5WWF0

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  pilC Legionella pneumophila strain ERS1305867

99.507

100

0.995
  pilC Pseudomonas stutzeri DSM 10701

54.208

99.507

0.539
  pilC Acinetobacter baumannii D1279779

53.58

99.754

0.534
  pilC Acinetobacter baylyi ADP1

52.84

99.754

0.527
  pilG Neisseria meningitidis 44/76-A

45.088

97.783

0.441
  pilG Neisseria gonorrhoeae MS11

44.836

97.783

0.438
  pilC Vibrio cholerae strain A1552

42

98.522

0.414
  pilC Vibrio campbellii strain DS40M4

38.916

100

0.389

Multiple sequence alignment