Detailed information    

insolico Bioinformatically predicted

Overview

Name   pilQ   Type   Machinery gene
Locus tag   ACI7YU_RS01925 Genome accession   NZ_CP173722
Coordinates   439951..442029 (+) Length   692 a.a.
NCBI ID   WP_406228076.1    Uniprot ID   -
Organism   Pseudomonas siliginis strain P     
Function   assembly of type IV pilus (predicted from homology)   
DNA binding and uptake

Genomic Context

Location: 434951..447029
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  ACI7YU_RS01905 (ACI7YU_01905) comM 437166..438230 (+) 1065 WP_041477568.1 pilus assembly protein PilM Machinery gene
  ACI7YU_RS01910 (ACI7YU_01910) - 438230..438796 (+) 567 WP_406228073.1 PilN domain-containing protein -
  ACI7YU_RS01915 (ACI7YU_01915) pilO 438793..439416 (+) 624 WP_041477570.1 type 4a pilus biogenesis protein PilO -
  ACI7YU_RS01920 (ACI7YU_01920) - 439413..439937 (+) 525 WP_406228075.1 pilus assembly protein PilP -
  ACI7YU_RS01925 (ACI7YU_01925) pilQ 439951..442029 (+) 2079 WP_406228076.1 type IV pilus secretin PilQ Machinery gene
  ACI7YU_RS01930 (ACI7YU_01930) aroK 442034..442552 (+) 519 WP_024011270.1 shikimate kinase AroK -
  ACI7YU_RS01935 (ACI7YU_01935) aroB 442705..443805 (+) 1101 WP_041477573.1 3-dehydroquinate synthase -
  ACI7YU_RS01940 (ACI7YU_01940) - 443816..445402 (+) 1587 WP_056787348.1 AAA family ATPase -

Sequence

Protein


Download         Length: 692 a.a.        Molecular weight: 74911.24 Da        Isoelectric Point: 5.4106

>NTDB_id=1073822 ACI7YU_RS01925 WP_406228076.1 439951..442029(+) (pilQ) [Pseudomonas siliginis strain P]
MNRIFSTLGFSLWIALVSPMVMAASLKALDVAALPGDRVELKLSFDGPPPQPKGYTTESPARIALDLPGVTSQMANKNLD
LGSGNARTATVAEAKDRTRLIVSLTQLAPYSTRVEGNNLFVVVGQGASTTAPRPAAVAPRAAMAAAAPARPAAQRSRAIR
GVDFQRGTEGEGNVVIDLSDPTIAPDIQEHDGKIILSFARTQLPEKLRVRLDVKDFATPVQFVNAAVTGDRAVISVEPSG
TFDYSTFQTDNKLTVSVRPMSIDDLQKRNADRQAYVGDKLSLNFQDIDVRSVLQLIADFTNLNLVASDTVQGGITLRLQN
VPWDQALDLVLKTKGLDKRKIGNVLLVAPADEIAARERQELESQKQIAELAPLRRELLQVNYAKAADIAKLFQSVTSAEA
KVDERGSITVDERTNNIIAYQTQDRLDELRRIVAQLDIPVRQVMIEARIVEANVDYDKSLGVRWGGSVQNKGNWNASGVN
GSSSTIGTPGSTGTNSPFVDMGTVNNTSGIGIAFITDNVLLDLELTAMEKTGNGEIVSQPKVVTSDKETAKILKGTEIPY
QEASSSGATSVSFKEASLSLEVTPQITPDNRIIMEVKVTKDEPDYLNKVQDVPPIKKNEVNAKVLVNDGETIVIGGVFSN
TQSKVVDKVPFLGDVPYLGRLFRRDVVSEKKSELLVFLTPRIMNNQAIAVSR

Nucleotide


Download         Length: 2079 bp        

>NTDB_id=1073822 ACI7YU_RS01925 WP_406228076.1 439951..442029(+) (pilQ) [Pseudomonas siliginis strain P]
ATGAACAGGATTTTCTCCACCCTCGGTTTTTCGCTATGGATAGCGCTGGTGTCACCGATGGTAATGGCGGCCAGTCTGAA
AGCGCTGGATGTTGCCGCGCTGCCGGGTGACCGCGTCGAACTGAAGCTGTCATTCGACGGCCCGCCACCACAGCCCAAGG
GCTACACAACCGAGTCACCGGCACGCATTGCACTGGATCTGCCGGGCGTGACCAGCCAGATGGCCAACAAGAATCTTGAT
CTGGGCAGCGGCAACGCGCGCACGGCCACGGTGGCCGAAGCCAAGGATCGCACGCGGCTGATTGTCAGCCTGACGCAGTT
GGCGCCTTACAGCACGCGGGTCGAGGGCAATAATCTGTTTGTGGTGGTCGGTCAGGGCGCCTCCACGACAGCGCCGCGCC
CGGCTGCCGTTGCACCGCGTGCCGCCATGGCCGCTGCGGCACCGGCCAGACCAGCCGCGCAACGCAGCAGGGCGATTCGC
GGTGTCGATTTCCAGCGCGGCACCGAGGGCGAGGGCAACGTAGTCATCGACCTCTCTGATCCGACCATCGCCCCGGATAT
CCAGGAGCACGATGGCAAGATCATCCTCAGCTTCGCCCGCACGCAATTGCCGGAAAAGCTGCGCGTGCGCCTCGACGTCA
AGGATTTCGCCACGCCGGTGCAGTTCGTCAATGCCGCGGTAACCGGTGATCGCGCAGTGATCAGCGTCGAGCCCAGCGGC
ACCTTCGACTATTCCACATTCCAGACCGACAACAAGCTCACCGTGAGCGTTCGGCCGATGAGCATCGACGACCTGCAAAA
GCGCAACGCCGACCGTCAGGCCTATGTCGGCGACAAGCTCTCGCTGAATTTTCAGGACATCGACGTGCGCTCGGTGCTGC
AACTGATCGCGGACTTCACCAATCTCAATCTGGTGGCGAGCGATACGGTGCAGGGCGGTATCACTTTGCGCCTGCAGAAC
GTGCCGTGGGATCAGGCACTGGATCTGGTGCTGAAAACCAAGGGGCTGGACAAGCGCAAGATCGGCAACGTGCTGCTGGT
CGCGCCGGCCGATGAAATTGCCGCTCGCGAGCGTCAGGAACTGGAATCGCAGAAGCAGATCGCCGAGTTGGCGCCCCTGC
GTCGTGAGCTGCTGCAAGTCAATTACGCCAAGGCAGCGGACATCGCCAAGCTGTTCCAGTCGGTGACCAGCGCCGAGGCC
AAGGTCGACGAGCGCGGTTCGATTACCGTCGATGAGCGAACCAACAACATCATTGCCTACCAGACCCAGGATCGCCTCGA
CGAACTCCGGCGGATCGTCGCCCAGCTGGATATTCCGGTGCGCCAGGTGATGATCGAGGCGCGCATCGTTGAGGCCAACG
TCGATTACGACAAGAGCCTGGGCGTGCGCTGGGGCGGTTCGGTGCAGAACAAGGGCAACTGGAACGCCTCCGGGGTCAAC
GGTTCGTCGAGCACTATCGGTACGCCGGGCAGCACCGGCACCAACTCGCCGTTCGTCGACATGGGCACGGTCAATAACAC
TTCGGGGATCGGCATTGCTTTCATCACCGACAACGTACTGCTGGATCTCGAACTGACGGCGATGGAAAAAACCGGCAATG
GCGAAATCGTCTCGCAGCCGAAGGTGGTCACCTCGGACAAGGAAACCGCGAAGATCCTCAAAGGCACCGAGATTCCCTAT
CAGGAGGCCAGCTCCAGCGGCGCTACCTCGGTGTCGTTCAAGGAGGCTTCGCTGTCACTGGAAGTCACCCCGCAGATCAC
CCCGGACAACCGCATCATCATGGAGGTCAAGGTCACCAAGGATGAGCCCGATTACCTGAACAAAGTGCAGGATGTGCCGC
CGATCAAGAAAAACGAGGTCAACGCCAAAGTGCTGGTAAATGACGGCGAGACCATCGTAATCGGCGGTGTTTTTTCAAAT
ACTCAGAGCAAGGTCGTAGATAAGGTGCCATTTCTTGGCGATGTGCCGTATCTTGGCCGCCTTTTCCGGCGTGATGTGGT
TTCGGAGAAAAAATCCGAGCTGCTGGTATTTCTCACTCCGCGTATCATGAATAACCAGGCGATTGCTGTGAGTCGTTGA

Domains

Predicted by InterproScan.

Secretin and TonB N terminus short domain (STN)

(303-349)

Bacterial type II/III secretion system short domain (Secretin_N)

(377-442)

AMIN domain (AMIN)

(37-122)

Bacterial type II and III secretion system protein (Secretin)

(527-683)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  pilQ Pseudomonas aeruginosa PAK

72.067

100

0.746
  comQ Acinetobacter baylyi ADP1

41.499

100

0.416
  pilQ Acinetobacter baumannii strain A118

37.652

100

0.403
  pilQ Acinetobacter baumannii D1279779

37.652

100

0.403
  pilQ Legionella pneumophila strain Lp02

37.997

98.121

0.373
  pilQ Legionella pneumophila strain ERS1305867

37.962

97.832

0.371

Multiple sequence alignment