Detailed information    

insolico Bioinformatically predicted

Overview

Name   pilQ   Type   Machinery gene
Locus tag   ACI514_RS23265 Genome accession   NZ_CP173611
Coordinates   5261527..5263608 (-) Length   693 a.a.
NCBI ID   WP_160055111.1    Uniprot ID   A0A7Z2QLP0
Organism   Pseudomonas sp. M20     
Function   assembly of type IV pilus (predicted from homology)   
DNA binding and uptake

Genomic Context

Location: 5256527..5268608
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  ACI514_RS23250 (ACI514_23235) - 5258148..5259740 (-) 1593 WP_160055117.1 AAA family ATPase -
  ACI514_RS23255 (ACI514_23240) aroB 5259751..5260851 (-) 1101 WP_405122463.1 3-dehydroquinate synthase -
  ACI514_RS23260 (ACI514_23245) aroK 5261004..5261522 (-) 519 WP_011332081.1 shikimate kinase AroK -
  ACI514_RS23265 (ACI514_23250) pilQ 5261527..5263608 (-) 2082 WP_160055111.1 type IV pilus secretin PilQ Machinery gene
  ACI514_RS23270 (ACI514_23255) - 5263622..5264149 (-) 528 WP_405122464.1 pilus assembly protein PilP -
  ACI514_RS23275 (ACI514_23260) pilO 5264146..5264769 (-) 624 WP_160055107.1 type 4a pilus biogenesis protein PilO -
  ACI514_RS23280 (ACI514_23265) - 5264766..5265332 (-) 567 WP_160055104.1 PilN domain-containing protein -
  ACI514_RS23285 (ACI514_23270) pilM 5265332..5266396 (-) 1065 WP_160055101.1 pilus assembly protein PilM Machinery gene

Sequence

Protein


Download         Length: 693 a.a.        Molecular weight: 75133.66 Da        Isoelectric Point: 5.8278

>NTDB_id=1073158 ACI514_RS23265 WP_160055111.1 5261527..5263608(-) (pilQ) [Pseudomonas sp. M20]
MNRIFSTLGFSLWIALMSPMVLAANLKTLDVAALPGDRVELKLSFDGPPPQPKGYTTESPARIALDLPGVASQLASKNLD
LGSGNARTATVVEAKDRTRLIVSLTQLAPYNTRVEGNNLFVVVGQGAPAAAPRPAAVAPRATAKAVAPAKAFVPKNRAIR
GVDFQRGTAGEGNVVIDLSDPTIAPDIQEHDGKIILSFARTQLPDKLRVRLDVKDFATPVQFVNAGVTADRTVITVEPSG
TYEYSTFQTDNKLTVSVRPMTVDDLQKRNADRQAYVGEKLSLNFQDIDVRSVLQLIADFTNLNLVASDTVQGGITLRLQN
VPWDQALDLVLKTKGLDKRKIGNVLLVAPADEIAARERQELESQKQIAELAPLRRELLQVNYAKAADIAKLFQSVTSAEA
KIDERGSITVDERTNNIIAYQTQDRLDELRRIVAQLDIPVRQVMIEARIVEANVDYDKSLGVRWGGSIQNKGNWNTSGVS
NGSSTTIGTPGSTSTNSPFVDMGTVGNTSGIGIAFITDNVLLDLELTAMEKTGNGEIVSQPKVVTSDKETAKILKGTEIP
YQEASSSGATSVSFKEASLSLEVTPQITPDNRIIMEVKVTKDEPDYLNKVQDVPPIKKNEVNAKVLVNDGETIVIGGVFS
NTQSKVVDKVPFLGDVPYLGRLFRRDVVSEKKSELLVFLTPRIMNNQAIAVSR

Nucleotide


Download         Length: 2082 bp        

>NTDB_id=1073158 ACI514_RS23265 WP_160055111.1 5261527..5263608(-) (pilQ) [Pseudomonas sp. M20]
ATGAACAGGATTTTCTCCACCCTCGGTTTTTCGCTATGGATAGCGCTGATGTCGCCGATGGTACTCGCGGCCAATCTGAA
GACGCTGGATGTGGCAGCGTTGCCGGGTGATCGCGTCGAGCTGAAGTTGTCGTTCGACGGCCCGCCGCCGCAACCCAAGG
GCTACACCACTGAATCGCCGGCGCGGATCGCCCTGGATCTGCCCGGTGTCGCCAGTCAGTTGGCCAGCAAGAATCTTGAT
CTGGGCAGTGGCAATGCGCGCACGGCCACGGTGGTCGAGGCCAAGGATCGGACTCGGTTGATCGTCAGCCTCACGCAACT
GGCGCCTTACAACACGCGGGTCGAAGGCAATAACCTGTTCGTGGTGGTCGGTCAGGGCGCACCGGCAGCGGCGCCGCGTC
CTGCTGCCGTTGCACCTCGTGCTACTGCTAAAGCGGTTGCGCCCGCCAAGGCCTTTGTGCCGAAAAACCGGGCGATTCGC
GGTGTGGACTTCCAGCGCGGTACGGCAGGTGAAGGCAATGTCGTCATCGACCTGTCCGACCCGACCATTGCCCCGGATAT
CCAGGAGCATGACGGCAAGATCATCCTCAGCTTTGCCCGCACGCAGTTGCCGGACAAGCTGCGGGTGCGCCTTGACGTCA
AGGATTTCGCCACCCCGGTGCAGTTCGTCAATGCCGGAGTGACCGCTGATCGCACGGTTATTACCGTTGAACCCAGTGGT
ACTTACGAGTACTCGACCTTCCAGACCGACAACAAACTGACCGTCAGCGTCCGCCCTATGACGGTCGATGACCTGCAAAA
GCGCAACGCCGATCGTCAGGCTTACGTCGGCGAAAAGCTTTCGCTGAATTTCCAGGACATTGATGTGCGCTCGGTGCTGC
AACTGATCGCCGACTTCACCAACCTCAACCTTGTCGCCAGCGACACGGTGCAGGGTGGCATCACTTTGCGCCTGCAAAAC
GTGCCGTGGGATCAGGCGCTGGATCTGGTGCTGAAAACCAAAGGTCTGGATAAACGCAAGATCGGCAACGTGCTGCTGGT
GGCTCCCGCCGATGAAATCGCTGCCCGCGAGCGCCAGGAACTGGAGTCGCAGAAACAGATCGCCGAGCTGGCGCCGTTGC
GCCGTGAGCTGCTGCAAGTGAACTACGCGAAGGCGGCGGACATCGCCAAGCTGTTTCAGTCGGTGACAAGCGCCGAAGCA
AAAATCGATGAACGTGGTTCGATCACCGTCGATGAGCGAACCAACAACATCATTGCCTATCAGACTCAGGATCGACTCGA
TGAGCTGCGGCGGATCGTGGCGCAACTGGATATTCCGGTGCGTCAGGTGATGATCGAAGCGCGAATTGTCGAAGCCAACG
TCGATTACGACAAAAGCCTCGGCGTGCGCTGGGGCGGGTCGATCCAGAACAAGGGTAACTGGAACACTTCGGGCGTCAGT
AATGGCTCATCGACCACCATCGGCACACCAGGCAGCACCAGCACCAACTCGCCGTTCGTCGATATGGGGACCGTGGGTAA
TACCTCGGGGATCGGCATTGCCTTCATCACTGACAATGTCTTGCTCGATCTCGAGCTGACAGCGATGGAGAAAACCGGCA
ACGGTGAAATCGTCTCGCAGCCGAAAGTGGTCACCTCCGACAAGGAAACCGCAAAAATCCTCAAAGGCACCGAGATTCCT
TATCAGGAAGCCAGCTCAAGCGGCGCGACGTCGGTGTCGTTCAAGGAGGCCTCGCTGTCACTGGAAGTCACCCCGCAGAT
CACCCCCGACAACCGCATCATCATGGAGGTCAAGGTCACCAAGGACGAACCGGATTACCTGAACAAAGTGCAGGATGTGC
CGCCGATCAAGAAAAACGAGGTCAACGCCAAGGTGCTGGTCAACGACGGCGAAACCATCGTGATTGGCGGTGTTTTCTCA
AATACTCAAAGCAAGGTCGTAGATAAGGTGCCATTTCTTGGCGATGTGCCGTATCTTGGCCGCCTTTTCCGGCGTGATGT
GGTTTCGGAGAAAAAATCCGAGCTGCTGGTATTTCTCACACCACGTATCATGAATAACCAGGCGATTGCTGTGAGTCGTT
GA

Domains

Predicted by InterproScan.

Bacterial type II/III secretion system short domain (Secretin_N)

(377-442)

Secretin and TonB N terminus short domain (STN)

(303-349)

AMIN domain (AMIN)

(37-122)

Bacterial type II and III secretion system protein (Secretin)

(528-684)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure
  AlphaFold DB A0A7Z2QLP0

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  pilQ Pseudomonas aeruginosa PAK

73.324

100

0.758
  comQ Acinetobacter baylyi ADP1

41.21

100

0.413
  pilQ Acinetobacter baumannii strain A118

38.005

100

0.407
  pilQ Acinetobacter baumannii D1279779

38.005

100

0.407
  pilQ Legionella pneumophila strain Lp02

38.166

97.547

0.372
  pilQ Legionella pneumophila strain ERS1305867

38.166

97.547

0.372

Multiple sequence alignment