Detailed information    

insolico Bioinformatically predicted

Overview

Name   pilM   Type   Machinery gene
Locus tag   ACI514_RS23285 Genome accession   NZ_CP173611
Coordinates   5265332..5266396 (-) Length   354 a.a.
NCBI ID   WP_160055101.1    Uniprot ID   A0A7Z2KPH3
Organism   Pseudomonas sp. M20     
Function   assembly of type IV pilus (predicted from homology)   
DNA binding and uptake

Genomic Context

Location: 5260332..5271396
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  ACI514_RS23260 (ACI514_23245) aroK 5261004..5261522 (-) 519 WP_011332081.1 shikimate kinase AroK -
  ACI514_RS23265 (ACI514_23250) pilQ 5261527..5263608 (-) 2082 WP_160055111.1 type IV pilus secretin PilQ Machinery gene
  ACI514_RS23270 (ACI514_23255) - 5263622..5264149 (-) 528 WP_405122464.1 pilus assembly protein PilP -
  ACI514_RS23275 (ACI514_23260) pilO 5264146..5264769 (-) 624 WP_160055107.1 type 4a pilus biogenesis protein PilO -
  ACI514_RS23280 (ACI514_23265) - 5264766..5265332 (-) 567 WP_160055104.1 PilN domain-containing protein -
  ACI514_RS23285 (ACI514_23270) pilM 5265332..5266396 (-) 1065 WP_160055101.1 pilus assembly protein PilM Machinery gene
  ACI514_RS23290 (ACI514_23275) - 5266615..5269059 (+) 2445 WP_405124243.1 penicillin-binding protein 1A -
  ACI514_RS23295 (ACI514_23280) - 5269380..5270648 (-) 1269 WP_038360054.1 malic enzyme-like NAD(P)-binding protein -

Sequence

Protein


Download         Length: 354 a.a.        Molecular weight: 38154.74 Da        Isoelectric Point: 4.8109

>NTDB_id=1073159 ACI514_RS23285 WP_160055101.1 5265332..5266396(-) (pilM) [Pseudomonas sp. M20]
MLGLFNKKTNTLLGIDISSTSVKLLELSRQGDRYRVEAYAVEPLPVNAVVEKNIAELEGVGQALSRVLVKARTGLKSVAV
AVAGSAVITKVIEMDAGLTDDELENQLKIEADQYIPYPLDEVAIDFEVQGVSPRNPERVNVLLAACRKENVEVREAALAL
AGLTARVVDVEAYALERSFGLLATQLAATQERLTVAVVDIGATMTTLSVLHNGKIIYTREQLFGGRQLTEEIQRRYGLTL
EQAGLAKKQGGLPDDYISEVLQPFREALVQQVSRSLQFFFASGQYNAVDHILLAGGTASVSGLDRLIEQRLNTPTQVANP
FANMALSSKVNAGALASDAPALMIACGLALRSFD

Nucleotide


Download         Length: 1065 bp        

>NTDB_id=1073159 ACI514_RS23285 WP_160055101.1 5265332..5266396(-) (pilM) [Pseudomonas sp. M20]
GTGCTGGGACTCTTCAATAAAAAGACCAATACGTTACTGGGGATCGACATCAGCTCTACGTCAGTGAAGCTGCTGGAACT
GAGCCGTCAGGGTGATCGCTACCGGGTCGAGGCGTACGCGGTAGAGCCATTGCCGGTCAACGCCGTGGTCGAAAAGAACA
TCGCCGAGCTTGAAGGCGTCGGCCAGGCCCTGAGCCGGGTGCTGGTCAAGGCGCGCACCGGGCTCAAAAGCGTGGCGGTC
GCGGTGGCCGGTTCCGCCGTGATCACCAAAGTCATCGAAATGGACGCCGGGCTGACCGACGACGAACTGGAAAACCAGCT
CAAGATCGAAGCCGACCAATACATTCCCTATCCGCTGGACGAAGTCGCCATCGATTTCGAAGTCCAGGGCGTTTCGCCGC
GCAACCCTGAACGAGTCAACGTGCTGCTGGCAGCTTGTCGCAAGGAAAACGTCGAAGTCCGTGAAGCGGCGCTGGCACTC
GCCGGGCTGACTGCGCGGGTCGTTGATGTCGAAGCCTATGCGCTGGAGCGCTCTTTCGGGTTGCTCGCCACGCAACTGGC
GGCCACGCAAGAGCGGCTCACTGTCGCCGTCGTCGATATCGGCGCGACCATGACCACCCTTAGCGTCTTGCACAACGGCA
AGATCATCTACACCCGCGAACAATTGTTTGGCGGTCGGCAATTGACCGAGGAGATCCAGCGGCGTTATGGCCTCACCCTC
GAGCAGGCGGGGCTGGCGAAAAAACAGGGCGGCCTGCCCGATGATTACATCAGCGAGGTCTTGCAGCCGTTTCGAGAGGC
GCTGGTCCAGCAGGTGTCGCGCTCGCTGCAGTTCTTCTTCGCTTCCGGGCAATACAACGCGGTCGACCACATTCTGCTGG
CTGGCGGCACGGCGTCGGTATCCGGGCTGGATCGGCTGATCGAGCAGCGCCTGAACACGCCAACGCAAGTGGCCAACCCG
TTTGCCAACATGGCCTTGAGCAGCAAGGTCAACGCCGGAGCGCTGGCCAGTGACGCGCCGGCCCTGATGATCGCCTGCGG
GCTCGCGCTCAGGAGTTTCGACTGA

Domains

Predicted by InterproScan.

Type IV pilus assembly protein PilM; (PilM_2)

(14-353)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure
  AlphaFold DB A0A7Z2KPH3

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  pilM Acinetobacter baumannii D1279779

56.215

100

0.562
  comM Acinetobacter nosocomialis M2

56.215

100

0.562
  comM Acinetobacter baylyi ADP1

55.085

100

0.551
  pilM Legionella pneumophila strain ERS1305867

47.175

100

0.472

Multiple sequence alignment