Detailed information    

insolico Bioinformatically predicted

Overview

Name   pilB   Type   Machinery gene
Locus tag   ACI3L6_RS11085 Genome accession   NZ_CP173383
Coordinates   2528011..2529696 (+) Length   561 a.a.
NCBI ID   WP_079400988.1    Uniprot ID   -
Organism   Vibrio campbellii strain LB314     
Function   type IV pilus biogenesis and function (predicted from homology)   
DNA binding and uptake

Genomic Context

Location: 2523011..2534696
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  ACI3L6_RS11065 (ACI3L6_11065) pdhR 2524602..2525369 (-) 768 WP_005428674.1 pyruvate dehydrogenase complex transcriptional repressor PdhR -
  ACI3L6_RS11070 (ACI3L6_11070) ampD 2525774..2526325 (-) 552 WP_079400990.1 1,6-anhydro-N-acetylmuramyl-L-alanine amidase AmpD -
  ACI3L6_RS11075 (ACI3L6_11075) nadC 2526418..2527305 (+) 888 WP_012128875.1 carboxylating nicotinate-nucleotide diphosphorylase -
  ACI3L6_RS11080 (ACI3L6_11080) - 2527571..2528011 (+) 441 WP_061065377.1 pilin -
  ACI3L6_RS11085 (ACI3L6_11085) pilB 2528011..2529696 (+) 1686 WP_079400988.1 type IV-A pilus assembly ATPase PilB Machinery gene
  ACI3L6_RS11090 (ACI3L6_11090) pilC 2529731..2530954 (+) 1224 WP_079400987.1 type II secretion system F family protein Machinery gene
  ACI3L6_RS11095 (ACI3L6_11095) pilD 2531027..2531896 (+) 870 WP_012128879.1 A24 family peptidase Machinery gene
  ACI3L6_RS11100 (ACI3L6_11100) coaE 2531897..2532511 (+) 615 WP_079400986.1 dephospho-CoA kinase -
  ACI3L6_RS11105 (ACI3L6_11105) zapD 2532539..2533279 (+) 741 WP_012128881.1 cell division protein ZapD -
  ACI3L6_RS11110 (ACI3L6_11110) yacG 2533342..2533536 (+) 195 WP_005534053.1 DNA gyrase inhibitor YacG -
  ACI3L6_RS11115 (ACI3L6_11115) rplS 2533926..2534279 (-) 354 WP_005379971.1 50S ribosomal protein L19 -

Sequence

Protein


Download         Length: 561 a.a.        Molecular weight: 62137.90 Da        Isoelectric Point: 5.1024

>NTDB_id=1071814 ACI3L6_RS11085 WP_079400988.1 2528011..2529696(+) (pilB) [Vibrio campbellii strain LB314]
MLTNLSTVLRQAGILTFSQEESLLEQVKASGISMPEALLSSGFFQSHELTEHLSSVFGLSCTSLNQYEYASLCQTLGLRD
LITRHNALPLNRTSSTLILAVADPTNLQAEDDFRFATGLQVELVLADFRELTAAIRRLYGRSLGQEKSGLKEINQDELAG
LVDISEDEIENIEDLSQDESPVSRYINQILLDAVRKGASDIHFEPYENMYRVRLRCDGILIEIQQPPSHLSRRLSARIKI
LSKLDIAERRLPQDGRIKLKLNQDTAIDMRVSTLPTLFGEKIVLRLLDSSSAALDINKLGYSDSQKQLYLDALRRPQGMI
LMTGPTGSGKTVSLYTGLNILNKPEINISTAEDPVEINLPGINQVQVQPKIGFGFAEALRSFLRQDPDVVMVGEIRDLDT
AEIAIKASQTGHLVLSTLHTNSAAETVIRLSNMGVESFNLASSLSLIIAQRLARKLCPYCRQPQEPNSQLQHIGITSTEQ
IFQANPDGCNECTHGYSGRTGIYEVMKFDESLSEALIKGASVHELEKLAIANGMQTLQMSGIEKLKQGITSFSELQRVLY
F

Nucleotide


Download         Length: 1686 bp        

>NTDB_id=1071814 ACI3L6_RS11085 WP_079400988.1 2528011..2529696(+) (pilB) [Vibrio campbellii strain LB314]
ATGCTAACCAACCTCTCAACGGTTCTTCGCCAAGCAGGAATACTGACTTTTTCCCAAGAAGAATCGTTACTTGAGCAAGT
AAAAGCTTCTGGCATTTCGATGCCGGAGGCTTTACTCAGTTCTGGCTTTTTCCAATCTCATGAATTAACAGAACACCTAA
GCTCTGTATTCGGGCTTTCTTGCACTTCTCTTAACCAATACGAATACGCTTCGCTATGCCAAACGCTTGGCTTACGCGAT
CTCATCACTCGCCATAACGCTTTGCCGCTTAACCGCACCTCTTCAACTTTAATTCTCGCGGTAGCTGACCCAACCAACCT
TCAAGCCGAAGATGACTTTCGTTTTGCAACCGGCTTGCAAGTCGAGCTTGTCCTCGCTGACTTTCGTGAGCTAACAGCAG
CCATTCGACGTTTATATGGCCGATCACTTGGGCAAGAGAAATCCGGTCTTAAAGAAATCAACCAAGATGAACTAGCGGGT
TTAGTTGATATTAGTGAGGATGAAATTGAGAACATCGAAGATCTCAGCCAAGATGAATCGCCGGTCAGTCGCTATATCAA
CCAGATTCTACTCGATGCTGTCCGTAAAGGGGCTTCCGATATTCACTTCGAGCCTTACGAAAACATGTACCGCGTGCGCT
TGCGTTGCGATGGCATCTTAATTGAAATTCAGCAGCCTCCGAGTCATTTAAGCCGTCGCCTTTCAGCTCGGATAAAAATC
CTATCTAAGCTCGATATTGCTGAACGTCGCTTACCTCAGGATGGACGAATCAAACTTAAGCTAAACCAAGACACCGCGAT
TGATATGCGCGTTTCTACATTGCCAACCCTATTTGGTGAGAAGATTGTTCTGCGTTTGCTCGACAGCAGTTCTGCAGCAT
TAGATATCAATAAGCTAGGTTATAGCGACTCACAGAAACAACTTTATTTGGATGCCCTGCGCCGTCCGCAAGGTATGATC
CTGATGACAGGCCCAACAGGTAGCGGTAAAACGGTCTCGCTCTATACTGGATTGAACATTCTTAATAAACCAGAGATCAA
TATCTCAACCGCTGAAGATCCCGTAGAGATTAACCTGCCCGGCATTAACCAAGTTCAAGTTCAGCCAAAGATCGGCTTTG
GTTTTGCAGAGGCGCTTCGCTCTTTCTTACGTCAAGACCCTGATGTCGTCATGGTGGGCGAGATCCGAGATCTCGACACA
GCAGAAATCGCGATCAAAGCATCACAGACTGGCCACTTAGTTCTCTCCACTTTGCACACAAACTCAGCTGCCGAGACTGT
TATTCGCTTGTCTAATATGGGCGTAGAAAGCTTTAACCTCGCCTCTTCTCTCAGTTTGATCATCGCCCAGCGTTTGGCTA
GAAAACTTTGTCCATATTGTAGACAACCTCAAGAGCCAAACAGCCAACTTCAACATATTGGAATCACCAGCACCGAACAG
ATCTTTCAAGCTAATCCCGACGGTTGTAACGAGTGTACCCATGGTTATTCAGGGCGAACCGGCATTTACGAAGTGATGAA
GTTTGATGAATCCCTTTCAGAAGCACTCATCAAAGGCGCCTCGGTACACGAATTAGAAAAACTCGCCATTGCCAATGGTA
TGCAAACCTTACAAATGTCTGGCATCGAAAAACTCAAACAAGGCATTACCAGCTTCAGCGAATTACAGCGCGTTCTCTAC
TTTTAA

Domains

Predicted by InterproScan.

Type II/IV secretion system protein (T2SSE)

(188-455)

Type II secretion system (T2SS), protein E, N-terminal domain (MshEN)

(38-142)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  pilB Vibrio campbellii strain DS40M4

98.93

100

0.989
  pilB Vibrio parahaemolyticus RIMD 2210633

90.731

100

0.907
  pilB Vibrio cholerae strain A1552

73.843

100

0.74
  pilB Legionella pneumophila strain ERS1305867

47.935

99.287

0.476
  pilB Acinetobacter baumannii D1279779

51.056

92.87

0.474
  pilB Acinetobacter baylyi ADP1

50.484

92.157

0.465
  pilF Neisseria gonorrhoeae MS11

43.794

100

0.44
  pilB/pilB1 Synechocystis sp. PCC 6803

36.7

100

0.389
  pilF Thermus thermophilus HB27

36.833

100

0.369
  pilB Deinococcus radiodurans R1 = ATCC 13939 = DSM 20539

36.559

99.465

0.364

Multiple sequence alignment