Detailed information    

insolico Bioinformatically predicted

Overview

Name   pilB   Type   Machinery gene
Locus tag   ACIRXM_RS11850 Genome accession   NZ_CP173234
Coordinates   2456865..2458289 (-) Length   474 a.a.
NCBI ID   WP_046097579.1    Uniprot ID   -
Organism   Avibacterium paragallinarum strain npAP/NC-USA/20240110/S1-1     
Function   type IV pilus biogenesis and function (predicted from homology)   
DNA binding and uptake

Genomic Context

Location: 2451865..2463289
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  ACIRXM_RS11825 (ACIRXM_11830) - 2452190..2453053 (-) 864 WP_410686629.1 phosphatidate cytidylyltransferase -
  ACIRXM_RS11830 (ACIRXM_11835) uppS 2453057..2453785 (-) 729 WP_410680654.1 polyprenyl diphosphate synthase -
  ACIRXM_RS11835 (ACIRXM_11840) ispC 2453939..2455135 (-) 1197 WP_410686630.1 1-deoxy-D-xylulose-5-phosphate reductoisomerase -
  ACIRXM_RS11840 (ACIRXM_11845) frr 2455317..2455874 (-) 558 WP_017807054.1 ribosome recycling factor -
  ACIRXM_RS11845 (ACIRXM_11850) pyrH 2455899..2456615 (-) 717 WP_035687020.1 UMP kinase -
  ACIRXM_RS11850 (ACIRXM_11855) pilB 2456865..2458289 (-) 1425 WP_046097579.1 GspE/PulE family protein Machinery gene
  ACIRXM_RS11855 (ACIRXM_11860) pilA 2458302..2458733 (-) 432 WP_410686631.1 pilin Machinery gene
  ACIRXM_RS11860 (ACIRXM_11865) ampD 2458873..2459427 (+) 555 WP_410680656.1 1,6-anhydro-N-acetylmuramyl-L-alanine amidase AmpD -
  ACIRXM_RS11865 (ACIRXM_11870) - 2459526..2459945 (+) 420 WP_410686632.1 hypothetical protein -
  ACIRXM_RS11870 (ACIRXM_11875) rppH 2460517..2461110 (+) 594 WP_410686634.1 RNA pyrophosphohydrolase -
  ACIRXM_RS11875 (ACIRXM_11880) - 2461113..2461904 (+) 792 WP_410686635.1 sulfite exporter TauE/SafE family protein -
  ACIRXM_RS11880 (ACIRXM_11885) lgt 2461914..2462714 (+) 801 WP_017807046.1 prolipoprotein diacylglyceryl transferase -

Sequence

Protein


Download         Length: 474 a.a.        Molecular weight: 53761.53 Da        Isoelectric Point: 5.3292

>NTDB_id=1071084 ACIRXM_RS11850 WP_046097579.1 2456865..2458289(-) (pilB) [Avibacterium paragallinarum strain npAP/NC-USA/20240110/S1-1]
MAEYSLDEDDIVYAVDGKCFEITPHLWQQNKQQKDILIRYLAVPLQEDEHTLWLGIDDLTNLSACESFSFLTGKIVEPIL
LSEQDLKSLLQKLSPTLVEEPALLDFYQSSISPYSENQPEKLDEPIIQILNKVFESAMQLNASDIHLEPQNEQLQIRFRI
DGVLQHQPPISKQMSQRLISRLKLLARLDLSETRLPQDGRFHFKTTFSDILDFRISTLPTYYGEKAVLRLQKNKPVTLDF
SELGMTAIQRKTLQNALSQPQGLILVTGPTGSGKSITLYTALEWLNQSEKHILTAEDPIEIELPGIIQTQINPQIGLDFN
RLLRTFLRQDPDIIMLGEIRDAESAAIALRAAQTGHLVLSTLHTNNAHSAIARLLQLGIQQYEIESCLLLVIAQRLVRKK
CLHKTKNCECYQGYSGRTGIYQFLQAIFDSNTGQISYQTDYPSLWDSASHKIAQGITDIAELERVLGRHTKAEL

Nucleotide


Download         Length: 1425 bp        

>NTDB_id=1071084 ACIRXM_RS11850 WP_046097579.1 2456865..2458289(-) (pilB) [Avibacterium paragallinarum strain npAP/NC-USA/20240110/S1-1]
ATGGCTGAGTATTCTTTAGACGAGGACGACATTGTATATGCTGTTGATGGAAAATGCTTTGAAATAACACCGCACTTATG
GCAACAAAATAAGCAGCAAAAAGATATTTTAATACGTTATTTAGCAGTACCTTTACAGGAGGATGAACATACACTTTGGC
TTGGTATTGATGACTTAACAAATCTATCGGCTTGTGAAAGTTTTTCATTTCTAACAGGAAAAATAGTTGAGCCGATTTTG
TTATCAGAACAAGATTTAAAATCGCTATTACAGAAGTTATCTCCCACATTAGTAGAAGAGCCGGCATTACTTGATTTTTA
TCAATCGTCTATTTCACCTTATAGCGAAAATCAACCAGAAAAACTTGATGAACCAATTATTCAAATACTAAATAAAGTTT
TTGAATCGGCAATGCAATTAAATGCCTCAGATATTCATTTAGAGCCACAAAATGAGCAATTACAGATCCGCTTCCGTATT
GACGGGGTATTGCAACATCAGCCTCCTATTTCTAAACAAATGAGCCAACGTTTGATTTCTCGTTTAAAACTTCTAGCTCG
TCTTGATCTTAGCGAAACTCGCTTACCACAAGATGGAAGATTTCATTTCAAAACGACATTTTCCGATATATTAGACTTCC
GTATATCGACATTACCTACATATTATGGAGAAAAAGCGGTACTACGTTTACAAAAAAATAAACCTGTTACCTTGGATTTT
TCTGAGCTGGGGATGACAGCGATACAACGAAAAACATTACAAAATGCTTTATCTCAACCTCAAGGACTCATTTTAGTAAC
GGGGCCAACTGGGAGTGGAAAAAGTATTACGCTCTATACAGCATTAGAATGGCTTAATCAATCGGAAAAACATATTCTAA
CAGCAGAAGATCCGATTGAAATAGAACTTCCAGGGATTATCCAAACGCAAATTAACCCACAAATTGGTTTAGATTTTAAC
CGTTTGTTGCGTACATTTTTACGTCAGGATCCAGACATTATTATGCTTGGTGAGATCCGTGATGCAGAAAGTGCAGCGAT
TGCGTTACGTGCAGCACAAACTGGACATTTAGTCCTTTCAACATTACATACAAATAATGCACATTCAGCTATTGCTCGCT
TGTTACAGCTTGGTATTCAACAATATGAAATTGAAAGTTGCTTATTATTAGTGATTGCCCAGCGTTTAGTCCGTAAAAAA
TGTCTACACAAAACGAAAAATTGCGAATGTTATCAAGGTTATAGTGGGCGTACTGGTATTTATCAATTTTTACAAGCTAT
TTTTGATTCAAATACAGGGCAAATTTCTTATCAAACCGATTATCCCTCATTATGGGATAGTGCTTCACATAAAATAGCAC
AAGGTATCACAGATATTGCTGAATTAGAACGAGTTTTAGGTAGGCATACAAAAGCTGAACTATAG

Domains

Predicted by InterproScan.

Type II/IV secretion system protein (T2SSE)

(132-399)

Type II secretion system (T2SS), protein E, N-terminal domain (MshEN)

(17-95)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  pilB Haemophilus influenzae Rd KW20

64.693

99.789

0.646
  pilB Haemophilus influenzae 86-028NP

63.924

100

0.639
  pilB Glaesserella parasuis strain SC1401

57.474

100

0.576
  pilB Legionella pneumophila strain ERS1305867

39.634

100

0.411
  pilB Vibrio campbellii strain DS40M4

40

100

0.409
  pilB Vibrio parahaemolyticus RIMD 2210633

39.794

100

0.407
  pilB Vibrio cholerae strain A1552

39.627

100

0.403
  pilB Acinetobacter baylyi ADP1

37.626

100

0.395
  pilF Neisseria gonorrhoeae MS11

37.317

100

0.376
  pilB Acinetobacter baumannii D1279779

36.495

100

0.373

Multiple sequence alignment