Detailed information NTG1070129

Bioinformatically predicted

Overview

• Name: comEA
• Type: Machinery gene
• Locus tag: ACIGXT_RS12465
• Genome accession: NZ_CP173038
• Coordinates: 2557756..2558370 (-)
• Length: 204 a.a.
• NCBI ID: WP_043867297.1
• Uniprot ID: -
• Organism: Bacillus velezensis strain Shannan.BV80-12
• Function: dsDNA binding to the cell surface (predicted from homology) DNA binding and uptake

Genomic Context

Location: 2552756..2563370

Locus tag	Gene name	Coordinates (strand)	Size (bp)	Protein ID	Product	Description
ACIGXT_RS12435 (ACIGXT_12435)	rpsT	2553056..2553322 (+)	267	WP_003152876.1	30S ribosomal protein S20	-
ACIGXT_RS12440 (ACIGXT_12440)	holA	2553339..2554380 (-)	1042	Protein_2403	DNA polymerase III subunit delta	-
ACIGXT_RS12445 (ACIGXT_12445)	-	2554420..2554574 (-)	155	Protein_2404	hypothetical protein	-
ACIGXT_RS12450 (ACIGXT_12450)	-	2554615..2554749 (+)	135	WP_003152870.1	YqzM family protein	-
ACIGXT_RS12455 (ACIGXT_12455)	comEC	2554768..2557119 (-)	2352	WP_404217255.1	DNA internalization-related competence protein ComEC/Rec2	Machinery gene
ACIGXT_RS12460 (ACIGXT_12460)	-	2557120..2557689 (-)	570	WP_003152868.1	ComE operon protein 2	-
ACIGXT_RS12465 (ACIGXT_12465)	comEA	2557756..2558370 (-)	615	WP_043867297.1	helix-hairpin-helix domain-containing protein	Machinery gene
ACIGXT_RS12470 (ACIGXT_12470)	comER	2558429..2559250 (+)	822	WP_044053476.1	late competence protein ComER	-
ACIGXT_RS12475 (ACIGXT_12475)	-	2559319..2560056 (-)	738	WP_014305450.1	class I SAM-dependent methyltransferase	-
ACIGXT_RS12480 (ACIGXT_12480)	rsfS	2560053..2560409 (-)	357	WP_003152864.1	ribosome silencing factor	-
ACIGXT_RS12485 (ACIGXT_12485)	yqeK	2560406..2560987 (-)	582	WP_003152863.1	bis(5'-nucleosyl)-tetraphosphatase (symmetrical) YqeK	-
ACIGXT_RS12490 (ACIGXT_12490)	-	2560977..2561546 (-)	570	WP_003152860.1	nicotinate-nucleotide adenylyltransferase	-
ACIGXT_RS12495 (ACIGXT_12495)	yhbY	2561557..2561847 (-)	291	WP_003152858.1	ribosome assembly RNA-binding protein YhbY	-
ACIGXT_RS12500 (ACIGXT_12500)	aroE	2561841..2562683 (-)	843	WP_025852995.1	shikimate dehydrogenase	-

Sequence

Protein

• Length: 204 a.a.
• Molecular weight: 22178.37 Da
• Isoelectric Point: 6.9685

>NTDB_id=1070129 ACIGXT_RS12465 WP_043867297.1 2557756..2558370(-) (comEA) [Bacillus velezensis strain Shannan.BV80-12]
MTKAMMTERMRRNLKILGACLAAGAVFICIWLFRSEKEEPVKQQSVLGQTASASVADKKDTADDKIMIDVKGAVRNPGVY
EMKAGDRVTQAIEKAGGLKKKADELNVNLAEQLQDGTIVYIPSEGEEGNHPKTAVGEKENAAVNINTASLDELQAISGVG
QKKAEAIIAYREENGRFQTAEDLMKVSGFGEKSFERIKTSITVK

Nucleotide

• Length: 615 bp

>NTDB_id=1070129 ACIGXT_RS12465 WP_043867297.1 2557756..2558370(-) (comEA) [Bacillus velezensis strain Shannan.BV80-12]
ATGACGAAAGCGATGATGACGGAGCGGATGCGGCGGAACCTGAAAATTCTGGGCGCTTGTCTTGCGGCAGGGGCAGTTTT
TATCTGTATTTGGCTGTTTCGTTCTGAAAAGGAAGAGCCGGTCAAACAGCAGTCCGTGCTCGGTCAAACAGCCTCCGCAT
CCGTCGCGGACAAAAAAGACACCGCAGACGATAAGATTATGATTGATGTGAAAGGAGCGGTCCGAAATCCCGGCGTGTAT
GAAATGAAAGCGGGAGACAGAGTGACGCAAGCCATTGAAAAAGCCGGCGGCCTGAAGAAAAAGGCGGATGAATTGAATGT
TAATTTAGCAGAGCAGCTGCAGGACGGGACGATTGTTTATATTCCGAGTGAAGGCGAAGAGGGGAATCATCCGAAAACAG
CAGTTGGTGAAAAGGAGAATGCAGCTGTCAATATCAATACGGCATCTCTGGACGAACTTCAGGCGATTTCCGGGGTGGGG
CAGAAAAAAGCGGAAGCCATTATCGCCTACCGAGAAGAAAACGGAAGGTTTCAGACTGCTGAAGATTTGATGAAGGTATC
AGGCTTTGGAGAAAAGTCATTTGAGCGGATAAAAACTTCCATTACGGTAAAGTGA

Domains

Predicted by InterproScan.

• Helix-hairpin-helix motif (HHH_3) (141-202)

• SLBB domain (SLBB) (68-122)

Similar proteins

Only experimentally validated proteins are listed.

Protein	Organism	Identities (%)	Coverage (%)	Ha-value
comEA	Bacillus subtilis subsp. subtilis str. 168	58.854	94.118	0.554
comEA/celA/cilE	Streptococcus pneumoniae TIGR4	36.019	100	0.373
comEA/celA/cilE	Streptococcus mitis SK321	35.545	100	0.368
comEA	Latilactobacillus sakei subsp. sakei 23K	31.897	100	0.363
comEA/celA/cilE	Streptococcus pneumoniae R6	35.749	100	0.363
comEA/celA/cilE	Streptococcus mitis NCTC 12261	35.749	100	0.363
comEA/celA/cilE	Streptococcus pneumoniae D39	35.749	100	0.363
comEA/celA/cilE	Streptococcus pneumoniae Rx1	35.749	100	0.363