Detailed information NTG1070127

Bioinformatically predicted

Overview

• Name: comEC
• Type: Machinery gene
• Locus tag: ACIGXT_RS12455
• Genome accession: NZ_CP173038
• Coordinates: 2554768..2557119 (-)
• Length: 783 a.a.
• NCBI ID: WP_404217255.1
• Uniprot ID: -
• Organism: Bacillus velezensis strain Shannan.BV80-12
• Function: ssDNA transport into the cell (predicted from homology) DNA binding and uptake

Genomic Context

Location: 2549768..2562119

Locus tag	Gene name	Coordinates (strand)	Size (bp)	Protein ID	Product	Description
ACIGXT_RS12420 (ACIGXT_12420)	-	2550131..2550469 (-)	339	WP_003152882.1	YqxA family protein	-
ACIGXT_RS12425 (ACIGXT_12425)	spoIIP	2550486..2551679 (-)	1194	WP_024085623.1	stage II sporulation protein P	-
ACIGXT_RS12430 (ACIGXT_12430)	gpr	2551747..2552853 (-)	1107	WP_003152878.1	GPR endopeptidase	-
ACIGXT_RS12435 (ACIGXT_12435)	rpsT	2553056..2553322 (+)	267	WP_003152876.1	30S ribosomal protein S20	-
ACIGXT_RS12440 (ACIGXT_12440)	holA	2553339..2554380 (-)	1042	Protein_2403	DNA polymerase III subunit delta	-
ACIGXT_RS12445 (ACIGXT_12445)	-	2554420..2554574 (-)	155	Protein_2404	hypothetical protein	-
ACIGXT_RS12450 (ACIGXT_12450)	-	2554615..2554749 (+)	135	WP_003152870.1	YqzM family protein	-
ACIGXT_RS12455 (ACIGXT_12455)	comEC	2554768..2557119 (-)	2352	WP_404217255.1	DNA internalization-related competence protein ComEC/Rec2	Machinery gene
ACIGXT_RS12460 (ACIGXT_12460)	-	2557120..2557689 (-)	570	WP_003152868.1	ComE operon protein 2	-
ACIGXT_RS12465 (ACIGXT_12465)	comEA	2557756..2558370 (-)	615	WP_043867297.1	helix-hairpin-helix domain-containing protein	Machinery gene
ACIGXT_RS12470 (ACIGXT_12470)	comER	2558429..2559250 (+)	822	WP_044053476.1	late competence protein ComER	-
ACIGXT_RS12475 (ACIGXT_12475)	-	2559319..2560056 (-)	738	WP_014305450.1	class I SAM-dependent methyltransferase	-
ACIGXT_RS12480 (ACIGXT_12480)	rsfS	2560053..2560409 (-)	357	WP_003152864.1	ribosome silencing factor	-
ACIGXT_RS12485 (ACIGXT_12485)	yqeK	2560406..2560987 (-)	582	WP_003152863.1	bis(5'-nucleosyl)-tetraphosphatase (symmetrical) YqeK	-
ACIGXT_RS12490 (ACIGXT_12490)	-	2560977..2561546 (-)	570	WP_003152860.1	nicotinate-nucleotide adenylyltransferase	-
ACIGXT_RS12495 (ACIGXT_12495)	yhbY	2561557..2561847 (-)	291	WP_003152858.1	ribosome assembly RNA-binding protein YhbY	-

Sequence

Protein

• Length: 783 a.a.
• Molecular weight: 86668.67 Da
• Isoelectric Point: 9.0078

>NTDB_id=1070127 ACIGXT_RS12455 WP_404217255.1 2554768..2557119(-) (comEC) [Bacillus velezensis strain Shannan.BV80-12]
MKYKYLLLPLAAVSATAGIAAAHVFWVLLLFLLYLLFIMIKTKQPAPVVVCLVSFCVYFFLYTVCDAANVTRYQAGSYTE
QAVITNIPKVDGAKMSAVIRTHDKEKWAASYKIRSLEEKRLIEQLEPGMRCTFTGSLEQPAHATIPGGFDYKEYLYSQQI
HWLFTVTSIQQCEKSKQPLFKLLSIRKNLISIIRNHVPESSAGIVEALTLGERFSIEDDILSAYQNLGVVHLMAISGMHV
GLITAGLFYALIRIGLTREKAGMLLLLFLPVYTLLSGAAPSVLRASLMLGFYIAGNLVKRGIHSSAALSLSYLLLLLFNP
YLLWQAGFQLSFAVSASLILSSSILKKAGKSRLAGLAMASFIAELSSLPFLLYHFQQISLASFPMNMVMVPFYTLFVIPV
SVIGFLLLLLSRQMGECLFNMFDLVMKPVHDFITYAASVDLFTMIVLKPDFLSLLLLVVSVFTLFAALEKGGFLKLRKSA
LFFCAVLAYLICRPYFSPWGEADMLDIGQGDSLFISAPHRKGTVMVDTGGVIAYPGESWKEKRHPYSIGEKVLIPFLNGK
GVKKLDALILTHADQDHIGEAGVLIKNHRVKRLIVPVGFVKEPKDQNILNMAKENNIPVAEAKRGDTITAGDLQFQVLSP
ESSDGKSKNDSSLVLWTVLGGVSWLLTGDLESDGETEVLKTYPKLKADILKAGHHGSKSSTSEAFLKQLQPEAALISAGK
ENRYHHPHEEVLDRLKAYSVNVLRTDISGTIQYRFEKGAGTFSVFPPYDIEETRAQEVKKTAD

Nucleotide

• Length: 2352 bp

>NTDB_id=1070127 ACIGXT_RS12455 WP_404217255.1 2554768..2557119(-) (comEC) [Bacillus velezensis strain Shannan.BV80-12]
ATGAAATATAAATACCTTCTTCTGCCTCTGGCGGCGGTTTCTGCAACTGCGGGAATTGCCGCCGCTCATGTCTTCTGGGT
TCTGCTCCTTTTTCTTCTGTATCTTCTCTTTATTATGATAAAAACAAAGCAGCCTGCTCCGGTTGTTGTCTGCCTCGTTT
CTTTTTGTGTTTATTTCTTTCTTTATACGGTTTGTGACGCTGCGAATGTAACGCGGTATCAGGCCGGCAGTTATACTGAA
CAGGCCGTCATCACTAATATTCCGAAGGTTGACGGAGCGAAAATGTCAGCCGTTATCCGTACACATGACAAGGAAAAATG
GGCGGCTTCGTACAAAATCCGGTCTCTTGAGGAAAAGAGACTCATTGAACAGCTTGAACCGGGGATGCGCTGCACGTTTA
CAGGCTCTCTGGAACAGCCTGCACATGCGACGATTCCCGGGGGTTTTGATTATAAGGAATATCTTTACTCTCAGCAGATT
CACTGGTTATTTACCGTGACTTCTATTCAGCAGTGTGAAAAATCCAAACAGCCGCTGTTTAAACTGCTGAGCATCAGAAA
AAATTTGATTTCGATCATTCGGAATCACGTGCCTGAATCTTCCGCCGGAATTGTTGAAGCGCTGACCTTAGGTGAAAGAT
TTTCTATAGAAGACGATATACTGAGTGCATATCAAAATTTGGGAGTCGTTCATTTAATGGCGATTTCCGGAATGCATGTC
GGTCTTATTACGGCGGGACTATTTTATGCTCTGATCAGAATCGGGCTGACAAGAGAAAAGGCGGGGATGTTGCTGCTGCT
GTTTTTGCCGGTCTATACGCTGCTGAGCGGTGCCGCCCCATCCGTATTGCGCGCATCCCTCATGCTGGGATTTTATATCG
CCGGAAATCTTGTTAAACGCGGCATTCATTCCTCTGCTGCATTGTCTCTGTCTTATCTGCTGCTCCTGCTGTTTAATCCT
TACCTCCTTTGGCAGGCGGGCTTCCAGCTTTCCTTTGCGGTAAGCGCCTCTTTAATTCTGTCATCCTCCATTTTAAAGAA
AGCAGGGAAAAGCAGACTTGCCGGGCTTGCGATGGCCTCATTCATCGCAGAGCTCAGCTCACTTCCGTTTCTTCTCTATC
ATTTTCAGCAAATTTCACTTGCCAGTTTTCCGATGAATATGGTGATGGTGCCATTTTATACGTTATTTGTCATTCCGGTT
TCTGTCATCGGTTTCCTTCTTCTTTTACTTTCAAGGCAGATGGGAGAATGTTTGTTTAATATGTTTGACCTTGTGATGAA
GCCTGTGCATGATTTCATTACATATGCGGCATCCGTTGATTTATTTACTATGATTGTGTTAAAGCCTGACTTTCTTTCCC
TTCTTCTGCTTGTGGTTTCCGTTTTTACGCTTTTTGCGGCTTTAGAAAAGGGAGGTTTTTTAAAACTCAGGAAATCGGCT
CTTTTTTTCTGCGCGGTTTTGGCTTATTTAATATGCCGTCCGTATTTCAGCCCATGGGGAGAAGCGGATATGCTTGATAT
CGGGCAGGGAGACTCGCTGTTTATAAGCGCGCCGCACCGCAAAGGGACCGTAATGGTTGATACAGGGGGAGTGATTGCTT
ATCCCGGAGAATCATGGAAAGAAAAACGCCACCCGTATTCTATCGGCGAGAAGGTTTTGATTCCGTTTTTAAACGGAAAA
GGGGTGAAAAAGCTGGATGCGCTGATTTTAACCCATGCGGATCAGGATCACATCGGGGAAGCCGGAGTGTTAATCAAAAA
TCATAGAGTCAAACGGTTAATTGTCCCCGTGGGATTCGTAAAAGAACCGAAGGATCAGAACATATTAAATATGGCGAAAG
AAAACAACATTCCCGTTGCCGAAGCAAAGCGGGGCGACACCATTACAGCCGGTGATCTTCAGTTTCAGGTGCTGTCTCCG
GAGTCGTCTGACGGAAAGAGTAAAAATGATTCGTCACTGGTGCTTTGGACGGTTTTAGGCGGAGTGAGCTGGCTTTTGAC
GGGAGATTTAGAATCGGACGGCGAAACAGAAGTGCTGAAAACGTATCCGAAACTGAAGGCTGATATATTGAAGGCGGGTC
ATCACGGCAGCAAAAGCTCAACGAGTGAAGCCTTTTTGAAACAGCTTCAGCCGGAAGCAGCGCTGATTTCAGCAGGAAAA
GAGAATCGATACCATCATCCGCACGAAGAAGTGCTGGATCGTTTGAAGGCGTACTCTGTCAATGTGCTTCGCACCGATAT
CAGCGGAACGATTCAATACAGATTTGAAAAAGGCGCCGGAACGTTTTCCGTCTTCCCTCCATATGATATAGAAGAAACCA
GGGCGCAAGAAGTAAAAAAGACTGCCGATTGA

Domains

Predicted by InterproScan.

• Metallo-beta-lactamase superfamily (Lactamase_B) (508-719)

• Domain of unknown function (DUF4131) (DUF4131) (25-171)

• Competence protein (Competence) (208-469)

Similar proteins

Only experimentally validated proteins are listed.

Protein	Organism	Identities (%)	Coverage (%)	Ha-value
comEC	Bacillus subtilis subsp. subtilis str. 168	56.477	98.595	0.557