Detailed information    

insolico Bioinformatically predicted

Overview

Name   pilA   Type   Machinery gene
Locus tag   RSP597_RS07355 Genome accession   NZ_CP172969
Coordinates   1532924..1533430 (-) Length   168 a.a.
NCBI ID   WP_064045722.1    Uniprot ID   -
Organism   Ralstonia solanacearum strain P597     
Function   type IV pilus biogenesis and function (predicted from homology)   
DNA binding and uptake

Genomic Context

Location: 1527924..1538430
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  RSP597_RS07335 (RSP597_007330) - 1528149..1528736 (+) 588 WP_064045708.1 DUF2946 family protein -
  RSP597_RS07340 (RSP597_007335) - 1528755..1530461 (-) 1707 WP_064045709.1 M48 family metallopeptidase -
  RSP597_RS07345 (RSP597_007340) moaC 1530563..1531039 (+) 477 WP_003261629.1 cyclic pyranopterin monophosphate synthase MoaC -
  RSP597_RS07350 (RSP597_007345) - 1531114..1532850 (-) 1737 WP_064045710.1 PglL family O-oligosaccharyltransferase -
  RSP597_RS07355 (RSP597_007350) pilA 1532924..1533430 (-) 507 WP_064045722.1 pilin Machinery gene
  RSP597_RS07360 (RSP597_007355) pilA 1533698..1534219 (-) 522 WP_064045711.1 pilin Machinery gene
  RSP597_RS07365 (RSP597_007360) - 1534403..1535101 (-) 699 WP_064045712.1 TerC family protein -
  RSP597_RS07370 (RSP597_007365) sucD 1535259..1536140 (-) 882 WP_003269430.1 succinate--CoA ligase subunit alpha -
  RSP597_RS07375 (RSP597_007370) sucC 1536219..1537385 (-) 1167 WP_003261636.1 ADP-forming succinate--CoA ligase subunit beta -
  RSP597_RS07380 (RSP597_007375) - 1537454..1538080 (-) 627 WP_064045713.1 DUF2889 domain-containing protein -

Sequence

Protein


Download         Length: 168 a.a.        Molecular weight: 17056.66 Da        Isoelectric Point: 9.9749

>NTDB_id=1069763 RSP597_RS07355 WP_064045722.1 1532924..1533430(-) (pilA) [Ralstonia solanacearum strain P597]
MKSMRHLNKRVQKGFTLIELMIVVAIVGILAAIAIPAYQDYTIRARVTEGLSLAAQAKSLVAENAANAQSDLSVGSSTFA
ATKNVSGLTINGTQAALGQITITYTTAAGNGTLALVPTSGGSTLAVNSAPGAAIMWTCYASGKATAASSIAANPSPTLLP
KYAPAECR

Nucleotide


Download         Length: 507 bp        

>NTDB_id=1069763 RSP597_RS07355 WP_064045722.1 1532924..1533430(-) (pilA) [Ralstonia solanacearum strain P597]
ATGAAGTCGATGCGTCATCTCAACAAGCGTGTCCAGAAGGGTTTCACGCTGATCGAACTGATGATCGTGGTCGCGATCGT
CGGTATTCTGGCTGCGATTGCCATTCCGGCCTATCAGGACTACACCATTCGTGCACGCGTGACCGAAGGTCTGTCGCTGG
CTGCACAGGCCAAGTCGCTGGTGGCTGAGAATGCTGCAAACGCTCAGAGTGACCTGTCTGTTGGTTCGTCGACGTTTGCG
GCTACCAAGAACGTGTCCGGTCTTACGATTAACGGGACTCAGGCGGCACTCGGCCAAATCACGATTACGTACACCACCGC
AGCGGGTAACGGTACGCTTGCCCTCGTCCCGACCTCGGGCGGTAGTACTCTGGCCGTGAATTCCGCTCCGGGTGCAGCTA
TCATGTGGACCTGCTACGCCTCTGGTAAGGCCACGGCTGCCAGCAGCATTGCTGCGAATCCCTCGCCGACCCTGCTGCCG
AAGTACGCTCCGGCAGAGTGCCGCTAA

Domains

Predicted by InterproScan.

Pilin (bacterial filament) (Pilin)

(44-168)

Prokaryotic N-terminal methylation motif (N_methyl)

(10-34)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  pilA Ralstonia pseudosolanacearum GMI1000

78.571

100

0.786
  pilA2 Legionella pneumophila str. Paris

51.553

95.833

0.494
  pilA2 Legionella pneumophila strain ERS1305867

51.553

95.833

0.494
  pilE Neisseria elongata subsp. glycolytica ATCC 29315

38.191

100

0.452
  comP Acinetobacter baylyi ADP1

42.69

100

0.435
  pilA/pilAI Pseudomonas stutzeri DSM 10701

34.659

100

0.363

Multiple sequence alignment