Detailed information    

insolico Bioinformatically predicted

Overview

Name   pilA   Type   Machinery gene
Locus tag   RSP597_RS07360 Genome accession   NZ_CP172969
Coordinates   1533698..1534219 (-) Length   173 a.a.
NCBI ID   WP_064045711.1    Uniprot ID   -
Organism   Ralstonia solanacearum strain P597     
Function   type IV pilus biogenesis and function (predicted from homology)   
DNA binding and uptake

Genomic Context

Location: 1528698..1539219
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  RSP597_RS07340 (RSP597_007335) - 1528755..1530461 (-) 1707 WP_064045709.1 M48 family metallopeptidase -
  RSP597_RS07345 (RSP597_007340) moaC 1530563..1531039 (+) 477 WP_003261629.1 cyclic pyranopterin monophosphate synthase MoaC -
  RSP597_RS07350 (RSP597_007345) - 1531114..1532850 (-) 1737 WP_064045710.1 PglL family O-oligosaccharyltransferase -
  RSP597_RS07355 (RSP597_007350) pilA 1532924..1533430 (-) 507 WP_064045722.1 pilin Machinery gene
  RSP597_RS07360 (RSP597_007355) pilA 1533698..1534219 (-) 522 WP_064045711.1 pilin Machinery gene
  RSP597_RS07365 (RSP597_007360) - 1534403..1535101 (-) 699 WP_064045712.1 TerC family protein -
  RSP597_RS07370 (RSP597_007365) sucD 1535259..1536140 (-) 882 WP_003269430.1 succinate--CoA ligase subunit alpha -
  RSP597_RS07375 (RSP597_007370) sucC 1536219..1537385 (-) 1167 WP_003261636.1 ADP-forming succinate--CoA ligase subunit beta -
  RSP597_RS07380 (RSP597_007375) - 1537454..1538080 (-) 627 WP_064045713.1 DUF2889 domain-containing protein -
  RSP597_RS07385 (RSP597_007380) recX 1538354..1538833 (-) 480 WP_064045714.1 recombination regulator RecX -

Sequence

Protein


Download         Length: 173 a.a.        Molecular weight: 17773.52 Da        Isoelectric Point: 10.0374

>NTDB_id=1069764 RSP597_RS07360 WP_064045711.1 1533698..1534219(-) (pilA) [Ralstonia solanacearum strain P597]
MASKHCRNRRRSQRGFTLIELMIVVAIIGILAAIAIPAYQDYTIRARVTEGLSLAAQAKALVVENAANSQSSLALGSASL
PASKNVSALNIDASTGEITVVYASAVTTGGANTLVLTPYSGSSSALANLRAGSAPPAMVLWVCAAAGKSMPVTTVTQNTT
PTLPPKYAPAECR

Nucleotide


Download         Length: 522 bp        

>NTDB_id=1069764 RSP597_RS07360 WP_064045711.1 1533698..1534219(-) (pilA) [Ralstonia solanacearum strain P597]
ATGGCATCGAAGCACTGCAGGAACAGGCGGCGCAGCCAGCGCGGCTTTACATTGATCGAGTTGATGATCGTGGTGGCGAT
CATCGGGATACTGGCGGCGATTGCGATTCCGGCGTACCAGGACTACACGATCCGGGCGCGCGTGACGGAAGGGCTGTCGC
TGGCTGCACAGGCCAAGGCGCTGGTGGTTGAGAATGCCGCCAACTCCCAGAGCAGTTTGGCGCTCGGCTCCGCATCATTG
CCGGCGAGCAAGAATGTCAGTGCTCTGAATATCGATGCGTCTACGGGTGAAATTACGGTGGTCTATGCGTCAGCTGTCAC
GACCGGAGGGGCGAACACGCTGGTTTTGACTCCATACTCGGGAAGCTCGTCAGCACTTGCTAACTTGCGCGCTGGCTCTG
CGCCGCCGGCAATGGTGTTGTGGGTGTGCGCTGCCGCCGGAAAGTCGATGCCGGTCACCACCGTCACCCAGAACACGACG
CCTACGTTGCCGCCGAAATATGCGCCTGCGGAATGCCGTTGA

Domains

Predicted by InterproScan.

Prokaryotic N-terminal methylation motif (N_methyl)

(10-35)

Pilin (bacterial filament) (Pilin)

(45-173)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  pilA Ralstonia pseudosolanacearum GMI1000

54.598

100

0.549
  pilA2 Legionella pneumophila str. Paris

47.273

95.376

0.451
  pilA2 Legionella pneumophila strain ERS1305867

46.667

95.376

0.445
  pilE Neisseria elongata subsp. glycolytica ATCC 29315

38.462

100

0.434
  comP Acinetobacter baylyi ADP1

41.615

93.064

0.387

Multiple sequence alignment