Detailed information    

insolico Bioinformatically predicted

Overview

Name   pilA   Type   Machinery gene
Locus tag   RSP673_RS04585 Genome accession   NZ_CP172582
Coordinates   982430..982936 (+) Length   168 a.a.
NCBI ID   WP_014617872.1    Uniprot ID   F6G3Z0
Organism   Ralstonia solanacearum P673     
Function   type IV pilus biogenesis and function (predicted from homology)   
DNA binding and uptake

Genomic Context

Location: 977430..987936
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  RSP673_RS04560 (RSP673_004560) - 977780..978406 (+) 627 WP_039550694.1 DUF2889 domain-containing protein -
  RSP673_RS04565 (RSP673_004565) sucC 978475..979641 (+) 1167 WP_039550692.1 ADP-forming succinate--CoA ligase subunit beta -
  RSP673_RS04570 (RSP673_004570) sucD 979720..980601 (+) 882 WP_003269430.1 succinate--CoA ligase subunit alpha -
  RSP673_RS04575 (RSP673_004575) - 980759..981457 (+) 699 WP_014617874.1 TerC family protein -
  RSP673_RS04580 (RSP673_004580) pilA 981641..982162 (+) 522 WP_014617873.1 pilin Machinery gene
  RSP673_RS04585 (RSP673_004585) pilA 982430..982936 (+) 507 WP_014617872.1 pilin Machinery gene
  RSP673_RS04590 (RSP673_004590) - 983021..984757 (+) 1737 WP_014617871.1 Wzy polymerase domain-containing protein -
  RSP673_RS04595 (RSP673_004595) moaC 984834..985310 (-) 477 WP_013206902.1 cyclic pyranopterin monophosphate synthase MoaC -
  RSP673_RS04600 (RSP673_004600) - 985412..987118 (+) 1707 WP_039550688.1 M48 family metallopeptidase -
  RSP673_RS04605 (RSP673_004605) - 987137..987724 (-) 588 WP_039550686.1 DUF2946 family protein -

Sequence

Protein


Download         Length: 168 a.a.        Molecular weight: 16882.51 Da        Isoelectric Point: 9.9749

>NTDB_id=1068125 RSP673_RS04585 WP_014617872.1 982430..982936(+) (pilA) [Ralstonia solanacearum P673]
MKSMRHLNKRVQKGFTLIELMIVVAIVGILAAIAIPAYQDYTIRARVTEGLSLAAQAKSLVAENAANAQSDLSVGSSTFA
ATKNVSGLAINATAAPAGQITITYTTAAGNGTLALVPTSGGNALVVSSAPGAAIMWTCYSAGKATAASSVAASPAATLLP
KYAPAECR

Nucleotide


Download         Length: 507 bp        

>NTDB_id=1068125 RSP673_RS04585 WP_014617872.1 982430..982936(+) (pilA) [Ralstonia solanacearum P673]
ATGAAGTCGATGCGTCATCTCAACAAGCGTGTCCAGAAGGGTTTCACGCTGATCGAACTGATGATCGTGGTCGCGATCGT
CGGTATTCTGGCTGCGATTGCCATTCCGGCCTATCAGGACTACACCATTCGTGCACGCGTGACCGAAGGTCTGTCGCTGG
CTGCGCAGGCCAAGTCGCTGGTGGCTGAGAATGCTGCAAACGCTCAGAGCGACCTGTCTGTGGGCTCGTCGACGTTTGCT
GCTACCAAGAACGTGTCCGGCCTTGCTATTAACGCGACTGCGGCACCGGCCGGCCAAATCACGATTACGTACACCACTGC
AGCGGGTAACGGTACGCTTGCCCTTGTCCCGACTTCGGGCGGTAATGCTTTGGTCGTGAGTTCCGCTCCGGGCGCAGCTA
TCATGTGGACCTGCTATTCTGCTGGTAAGGCCACGGCTGCCAGCAGCGTTGCTGCGAGCCCCGCGGCGACCCTGCTGCCG
AAGTATGCTCCGGCAGAATGCCGCTAA

Domains

Predicted by InterproScan.

Pilin (bacterial filament) (Pilin)

(44-168)

Prokaryotic N-terminal methylation motif (N_methyl)

(10-34)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure
  AlphaFold DB F6G3Z0

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  pilA Ralstonia pseudosolanacearum GMI1000

79.167

100

0.792
  pilA2 Legionella pneumophila str. Paris

52.174

95.833

0.5
  pilA2 Legionella pneumophila strain ERS1305867

52.174

95.833

0.5
  pilE Neisseria elongata subsp. glycolytica ATCC 29315

38.265

100

0.446
  comP Acinetobacter baylyi ADP1

42.353

100

0.429
  pilA/pilAI Pseudomonas stutzeri DSM 10701

36.095

100

0.363

Multiple sequence alignment