Detailed information    

insolico Bioinformatically predicted

Overview

Name   pilA   Type   Machinery gene
Locus tag   RSP673_RS04580 Genome accession   NZ_CP172582
Coordinates   981641..982162 (+) Length   173 a.a.
NCBI ID   WP_014617873.1    Uniprot ID   F6G3Z1
Organism   Ralstonia solanacearum P673     
Function   type IV pilus biogenesis and function (predicted from homology)   
DNA binding and uptake

Genomic Context

Location: 976641..987162
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  RSP673_RS04555 (RSP673_004555) recX 977027..977506 (+) 480 WP_039550696.1 recombination regulator RecX -
  RSP673_RS04560 (RSP673_004560) - 977780..978406 (+) 627 WP_039550694.1 DUF2889 domain-containing protein -
  RSP673_RS04565 (RSP673_004565) sucC 978475..979641 (+) 1167 WP_039550692.1 ADP-forming succinate--CoA ligase subunit beta -
  RSP673_RS04570 (RSP673_004570) sucD 979720..980601 (+) 882 WP_003269430.1 succinate--CoA ligase subunit alpha -
  RSP673_RS04575 (RSP673_004575) - 980759..981457 (+) 699 WP_014617874.1 TerC family protein -
  RSP673_RS04580 (RSP673_004580) pilA 981641..982162 (+) 522 WP_014617873.1 pilin Machinery gene
  RSP673_RS04585 (RSP673_004585) pilA 982430..982936 (+) 507 WP_014617872.1 pilin Machinery gene
  RSP673_RS04590 (RSP673_004590) - 983021..984757 (+) 1737 WP_014617871.1 Wzy polymerase domain-containing protein -
  RSP673_RS04595 (RSP673_004595) moaC 984834..985310 (-) 477 WP_013206902.1 cyclic pyranopterin monophosphate synthase MoaC -
  RSP673_RS04600 (RSP673_004600) - 985412..987118 (+) 1707 WP_039550688.1 M48 family metallopeptidase -

Sequence

Protein


Download         Length: 173 a.a.        Molecular weight: 17727.44 Da        Isoelectric Point: 10.2418

>NTDB_id=1068124 RSP673_RS04580 WP_014617873.1 981641..982162(+) (pilA) [Ralstonia solanacearum P673]
MASKHSRNRRRGQRGFTLIELMIVVAIIGILAAIAIPAYQDYTIRARVTEGLSLAAQAKALVVENAANSQSSLALGSASL
PASKNVSALNIDASTGEITVVYASAVTTGGANTLVLTPYSGSSSALANLRAGSAPPAMVLWVCAAAGKSMPVTTVTQNTT
PTLPPKYAPAECR

Nucleotide


Download         Length: 522 bp        

>NTDB_id=1068124 RSP673_RS04580 WP_014617873.1 981641..982162(+) (pilA) [Ralstonia solanacearum P673]
ATGGCATCGAAGCACAGCAGGAACAGGCGGCGCGGTCAGCGCGGCTTTACGTTGATCGAGTTGATGATCGTGGTGGCGAT
CATCGGGATACTGGCGGCGATTGCGATTCCGGCGTACCAGGACTACACGATCCGGGCGCGCGTGACGGAAGGGCTGTCGC
TGGCTGCACAGGCCAAGGCGCTGGTGGTTGAGAATGCCGCCAACTCCCAGAGCAGTTTGGCGCTCGGCTCCGCATCATTG
CCGGCGAGCAAGAATGTCAGTGCTCTGAATATCGATGCGTCTACGGGTGAGATTACGGTGGTCTATGCGTCAGCTGTCAC
GACCGGAGGCGCGAACACGCTGGTTTTGACTCCATACTCGGGAAGCTCGTCAGCACTTGCTAACTTGCGCGCTGGCTCTG
CGCCGCCGGCAATGGTGTTGTGGGTGTGCGCTGCCGCCGGAAAGTCGATGCCGGTCACCACCGTCACCCAGAACACGACG
CCTACGTTGCCGCCGAAATATGCGCCTGCGGAATGCCGTTGA

Domains

Predicted by InterproScan.

Pilin (bacterial filament) (Pilin)

(45-173)

Prokaryotic N-terminal methylation motif (N_methyl)

(10-35)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure
  AlphaFold DB F6G3Z1

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  pilA Ralstonia pseudosolanacearum GMI1000

54.545

100

0.555
  pilA2 Legionella pneumophila str. Paris

47.273

95.376

0.451
  pilA2 Legionella pneumophila strain ERS1305867

46.667

95.376

0.445
  pilE Neisseria elongata subsp. glycolytica ATCC 29315

38.462

100

0.434
  comP Acinetobacter baylyi ADP1

41.615

93.064

0.387

Multiple sequence alignment