Detailed information    

insolico Bioinformatically predicted

Overview

Name   pilB   Type   Machinery gene
Locus tag   ACG2XO_RS02870 Genome accession   NZ_CP172558
Coordinates   627618..629324 (+) Length   568 a.a.
NCBI ID   WP_410499330.1    Uniprot ID   -
Organism   Chitinibacter sp. S2-10     
Function   power the assembly of type IV pilus (predicted from homology)   
DNA binding and uptake

Genomic Context

Location: 622618..634324
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  ACG2XO_RS02840 (ACG2XO_02840) rplS 623039..623419 (-) 381 WP_180306044.1 50S ribosomal protein L19 -
  ACG2XO_RS02845 (ACG2XO_02845) trmD 623458..624219 (-) 762 WP_410500225.1 tRNA (guanosine(37)-N1)-methyltransferase TrmD -
  ACG2XO_RS02850 (ACG2XO_02850) rimM 624265..624768 (-) 504 WP_410499327.1 ribosome maturation factor RimM -
  ACG2XO_RS02855 (ACG2XO_02855) rpsP 624821..625066 (-) 246 WP_157670806.1 30S ribosomal protein S16 -
  ACG2XO_RS02860 (ACG2XO_02860) ffh 625166..626509 (-) 1344 WP_410499328.1 signal recognition particle protein -
  ACG2XO_RS02865 (ACG2XO_02865) - 626591..627397 (+) 807 WP_410499329.1 inner membrane protein YpjD -
  ACG2XO_RS02870 (ACG2XO_02870) pilB 627618..629324 (+) 1707 WP_410499330.1 type IV-A pilus assembly ATPase PilB Machinery gene
  ACG2XO_RS02875 (ACG2XO_02875) pilC 629338..630561 (+) 1224 WP_410499331.1 type II secretion system F family protein Machinery gene
  ACG2XO_RS02880 (ACG2XO_02880) pilD 630561..631418 (+) 858 WP_410499332.1 prepilin peptidase Machinery gene
  ACG2XO_RS02885 (ACG2XO_02885) coaE 631425..632030 (+) 606 WP_410499333.1 dephospho-CoA kinase -
  ACG2XO_RS02890 (ACG2XO_02890) zapD 632067..632825 (+) 759 WP_410499334.1 cell division protein ZapD -
  ACG2XO_RS02895 (ACG2XO_02895) - 632867..633010 (+) 144 WP_410499335.1 DNA gyrase inhibitor YacG -
  ACG2XO_RS02900 (ACG2XO_02900) - 633118..633936 (-) 819 WP_410499336.1 symmetrical bis(5'-nucleosyl)-tetraphosphatase -

Sequence

Protein


Download         Length: 568 a.a.        Molecular weight: 62516.96 Da        Isoelectric Point: 5.4274

>NTDB_id=1068024 ACG2XO_RS02870 WP_410499330.1 627618..629324(+) (pilB) [Chitinibacter sp. S2-10]
MSTPQISGLARLLVQHGRLSESDAEALHLAANSNKTTFIEQLTQSKKLSPREVAEFSAQTFGYPLLDLDQIDSNYLPQNV
LDNNVMRLQRLVPLFKRGTKLFIGLSDITNLEAVEKVRFQTNSQVEPIIVEDNKLVILLNKTIEASGANLKEMVIDEADL
ALEGGETEPTAAETQMGQDIDDAPVVKYLQKTLMDAINGGASDIHFEPYEKFYRIRYRIDGVLREIAQPPLAIKEKISSR
IKVISKLDISEKRVPQDGRMKLVLSKSRAIDFRVSTLPTMHGEKIVMRILDPSSATLGIDALGYDPDQKAIILDAIQRPY
GMVLVTGPTGSGKTVSLYTCLNLLNKPDINISTAEDPCEINLPGINQVNVNDKAGLTFSAALKAFLRQDPDIIMVGEIRD
LETADIAIKAAQTGHMVFSTLHTNDAPTTLTRMLNMGIQPFNIASSVILITAQRLARRLCSCKKHVDIPAQALLDAGFSQ
EELDGSWEPYGPVGCELCKNSGYKGRVGIYQVMPITDDINRLIMNHGNAIDIADQARKEGVLSLREAGLRKVKQGLTSLE
EIMAVTNI

Nucleotide


Download         Length: 1707 bp        

>NTDB_id=1068024 ACG2XO_RS02870 WP_410499330.1 627618..629324(+) (pilB) [Chitinibacter sp. S2-10]
ATGAGCACCCCGCAAATTTCCGGCCTTGCCCGCTTACTGGTTCAGCATGGCCGACTGAGCGAGTCTGATGCTGAAGCACT
GCACCTAGCAGCCAATAGCAATAAAACCACATTTATTGAGCAATTGACTCAAAGCAAGAAATTATCACCGCGCGAAGTCG
CAGAATTCTCTGCTCAGACTTTCGGTTATCCTTTGCTCGACCTAGATCAAATTGATTCCAATTATCTTCCGCAAAACGTC
CTCGACAATAACGTCATGCGACTGCAACGCCTAGTGCCGCTTTTCAAGCGAGGCACCAAGCTATTTATCGGACTATCCGA
CATCACCAACCTGGAAGCCGTAGAAAAAGTCCGTTTTCAGACCAACTCACAAGTAGAACCAATTATTGTCGAAGACAATA
AGCTCGTTATTCTACTCAATAAAACAATTGAGGCATCTGGCGCCAACCTCAAAGAGATGGTGATCGACGAGGCAGATCTG
GCACTTGAGGGTGGAGAAACCGAACCCACAGCCGCCGAAACGCAAATGGGACAAGATATTGATGACGCCCCTGTCGTTAA
ATACCTGCAAAAAACGCTAATGGATGCAATCAACGGCGGAGCTTCGGATATTCACTTCGAGCCCTACGAAAAGTTTTATC
GCATTCGCTACCGAATCGACGGCGTTTTGCGCGAAATTGCGCAACCCCCTCTGGCCATCAAGGAAAAAATTTCATCGCGC
ATTAAGGTGATCTCCAAGCTGGATATTTCCGAAAAGCGCGTACCGCAAGACGGCCGCATGAAACTGGTTTTATCCAAATC
AAGGGCAATTGACTTCCGGGTCTCTACCCTGCCAACGATGCATGGTGAAAAAATTGTCATGCGGATTTTGGATCCATCCT
CAGCCACGCTAGGTATTGACGCACTCGGTTACGACCCCGATCAGAAAGCCATCATTCTCGATGCTATCCAGCGCCCCTAT
GGTATGGTTCTGGTTACCGGCCCAACCGGCTCGGGTAAAACGGTATCGCTTTACACCTGCCTCAACCTACTTAACAAACC
GGACATCAATATCTCGACTGCAGAGGATCCTTGCGAAATCAACTTGCCGGGGATCAATCAAGTCAATGTAAACGACAAGG
CAGGCCTCACCTTTTCGGCGGCACTTAAAGCCTTTCTGCGCCAAGACCCTGACATCATCATGGTTGGTGAGATTCGTGAT
CTGGAAACGGCAGATATCGCGATCAAAGCAGCTCAGACTGGCCATATGGTATTTTCAACACTGCACACCAATGATGCCCC
CACGACATTGACCCGGATGCTCAATATGGGCATTCAGCCTTTTAACATTGCATCATCCGTTATTTTAATTACCGCACAAC
GGCTAGCTCGACGCTTATGCAGCTGCAAAAAACATGTTGATATTCCGGCACAAGCCCTGCTCGACGCTGGCTTTTCTCAG
GAAGAGCTGGACGGGAGCTGGGAGCCTTACGGTCCGGTAGGGTGCGAGCTCTGCAAAAACTCCGGCTACAAAGGCCGGGT
CGGTATTTATCAGGTCATGCCCATTACCGACGACATCAACCGTCTTATCATGAATCATGGCAATGCAATCGACATTGCCG
ATCAAGCTCGTAAAGAAGGTGTACTTAGCTTGCGCGAAGCCGGATTACGAAAAGTAAAACAAGGATTGACCTCGCTTGAA
GAAATCATGGCGGTCACAAACATCTAG

Domains

Predicted by InterproScan.

Type II secretion system (T2SS), protein E, N-terminal domain (MshEN)

(42-140)

Type II/IV secretion system protein (T2SSE)

(191-458)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  pilB Acinetobacter baumannii D1279779

55.009

100

0.551
  pilF Neisseria gonorrhoeae MS11

54.707

99.12

0.542
  pilB Acinetobacter baylyi ADP1

54.321

99.824

0.542
  pilB Legionella pneumophila strain ERS1305867

52.273

100

0.526
  pilB Vibrio cholerae strain A1552

46.397

100

0.465
  pilB Vibrio parahaemolyticus RIMD 2210633

45.745

99.296

0.454
  pilB Vibrio campbellii strain DS40M4

45.39

99.296

0.451
  pilF Thermus thermophilus HB27

38.004

100

0.382
  pilB Deinococcus radiodurans R1 = ATCC 13939 = DSM 20539

38.121

99.296

0.379

Multiple sequence alignment